Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepato-renal toxicity via augmentation of the antioxidant system in rats
Summary: Arsenicosis remains a global health concern due to devastating health effects. Clerodendrum volubile and vernonia amygdalina have tremendous bioactivities against oxidative stress-related diseases. The study, therefore, appraised the effects of flavonoids fractions from C. volubile and V. a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-02-01
|
| Series: | Clinical Nutrition Open Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667268520300036 |
| _version_ | 1818447134709514240 |
|---|---|
| author | Regina Ngozi Ugbaja Temilade Fola Akinhanmi Adewale Segun James Emmanuel Ifeanyichukwu Ugwor Adetola Adedayo Babalola Emmanuel Obinna Ezenandu Victory Chukwudalu Ugbaja Esther Ayobami Emmanuel |
| author_facet | Regina Ngozi Ugbaja Temilade Fola Akinhanmi Adewale Segun James Emmanuel Ifeanyichukwu Ugwor Adetola Adedayo Babalola Emmanuel Obinna Ezenandu Victory Chukwudalu Ugbaja Esther Ayobami Emmanuel |
| author_sort | Regina Ngozi Ugbaja |
| collection | DOAJ |
| description | Summary: Arsenicosis remains a global health concern due to devastating health effects. Clerodendrum volubile and vernonia amygdalina have tremendous bioactivities against oxidative stress-related diseases. The study, therefore, appraised the effects of flavonoids fractions from C. volubile and V. amygdalina (FCV and FVA respectively) against arsenic-induced oxidative stress in rats. Thirty male Wistar rats (120 ± 10 g) were divided into six groups of five each; Control (distilled water), arsenic alone (40 ppm sodium arsenite), arsenic + FCV (100 mg/kg), arsenic + FVA (100 mg/kg), arsenic + FCV and FVA (50 mg/kg each), and arsenic + vitamin C (100 mg/kg). The treatment commenced after four-week long arsenic exposure and lasted another four weeks. Blood, liver and kidneys of the rats were collected after sacrifice following an overnight fast. Arsenic caused significant (p<0.05) reductions in the total thiols levels in the plasma, liver, and kidneys, as well as the lowering of catalase and glutathione peroxidase activities. Contrariwise, malondialdehyde and nitric oxide levels, as well as superoxide dismutase activities increased in the non-treated arsenic exposed group. FCV and FVA, both singly or in combination, abrogated the oxidative stress indices and enhanced the antioxidant species in the treated groups. Groups treated with vitamin C also showed improved antioxidant status with concomitant reductions in oxidative stress markers. This study concludes that flavonoids fractions from C. volubile and V. amygdalina could be a viable weapon against arsenic-induced hepato-renal oxidative stress in rats. |
| first_indexed | 2024-12-14T19:58:48Z |
| format | Article |
| id | doaj.art-45fdd160b4354a89bdd561a36f2df3ff |
| institution | Directory Open Access Journal |
| issn | 2667-2685 |
| language | English |
| last_indexed | 2024-12-14T19:58:48Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Clinical Nutrition Open Science |
| spelling | doaj.art-45fdd160b4354a89bdd561a36f2df3ff2022-12-21T22:49:13ZengElsevierClinical Nutrition Open Science2667-26852021-02-01351225Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepato-renal toxicity via augmentation of the antioxidant system in ratsRegina Ngozi Ugbaja0Temilade Fola Akinhanmi1Adewale Segun James2Emmanuel Ifeanyichukwu Ugwor3Adetola Adedayo Babalola4Emmanuel Obinna Ezenandu5Victory Chukwudalu Ugbaja6Esther Ayobami Emmanuel7Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, Nigeria; Department of Chemical Sciences, (Biochemistry Program), Faculty of Sciences, Augustine University, Ilara-Epe, Lagos State, Nigeria; Corresponding author.Department of Chemistry, College of Physical Sciences, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, NigeriaDepartment of Chemistry, College of Physical Sciences, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, NigeriaSummary: Arsenicosis remains a global health concern due to devastating health effects. Clerodendrum volubile and vernonia amygdalina have tremendous bioactivities against oxidative stress-related diseases. The study, therefore, appraised the effects of flavonoids fractions from C. volubile and V. amygdalina (FCV and FVA respectively) against arsenic-induced oxidative stress in rats. Thirty male Wistar rats (120 ± 10 g) were divided into six groups of five each; Control (distilled water), arsenic alone (40 ppm sodium arsenite), arsenic + FCV (100 mg/kg), arsenic + FVA (100 mg/kg), arsenic + FCV and FVA (50 mg/kg each), and arsenic + vitamin C (100 mg/kg). The treatment commenced after four-week long arsenic exposure and lasted another four weeks. Blood, liver and kidneys of the rats were collected after sacrifice following an overnight fast. Arsenic caused significant (p<0.05) reductions in the total thiols levels in the plasma, liver, and kidneys, as well as the lowering of catalase and glutathione peroxidase activities. Contrariwise, malondialdehyde and nitric oxide levels, as well as superoxide dismutase activities increased in the non-treated arsenic exposed group. FCV and FVA, both singly or in combination, abrogated the oxidative stress indices and enhanced the antioxidant species in the treated groups. Groups treated with vitamin C also showed improved antioxidant status with concomitant reductions in oxidative stress markers. This study concludes that flavonoids fractions from C. volubile and V. amygdalina could be a viable weapon against arsenic-induced hepato-renal oxidative stress in rats.http://www.sciencedirect.com/science/article/pii/S2667268520300036Clerodendrum volubileVernonia amygdalinahepato-protectionreno-protetionarsenicantioxidants |
| spellingShingle | Regina Ngozi Ugbaja Temilade Fola Akinhanmi Adewale Segun James Emmanuel Ifeanyichukwu Ugwor Adetola Adedayo Babalola Emmanuel Obinna Ezenandu Victory Chukwudalu Ugbaja Esther Ayobami Emmanuel Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepato-renal toxicity via augmentation of the antioxidant system in rats Clinical Nutrition Open Science Clerodendrum volubile Vernonia amygdalina hepato-protection reno-protetion arsenic antioxidants |
| title | Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepato-renal toxicity via augmentation of the antioxidant system in rats |
| title_full | Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepato-renal toxicity via augmentation of the antioxidant system in rats |
| title_fullStr | Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepato-renal toxicity via augmentation of the antioxidant system in rats |
| title_full_unstemmed | Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepato-renal toxicity via augmentation of the antioxidant system in rats |
| title_short | Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepato-renal toxicity via augmentation of the antioxidant system in rats |
| title_sort | flavonoid rich fractions from clerodendrum volubile and vernonia amygdalina extenuates arsenic invoked hepato renal toxicity via augmentation of the antioxidant system in rats |
| topic | Clerodendrum volubile Vernonia amygdalina hepato-protection reno-protetion arsenic antioxidants |
| url | http://www.sciencedirect.com/science/article/pii/S2667268520300036 |
| work_keys_str_mv | AT reginangoziugbaja flavonoidrichfractionsfromclerodendrumvolubileandvernoniaamygdalinaextenuatesarsenicinvokedhepatorenaltoxicityviaaugmentationoftheantioxidantsysteminrats AT temiladefolaakinhanmi flavonoidrichfractionsfromclerodendrumvolubileandvernoniaamygdalinaextenuatesarsenicinvokedhepatorenaltoxicityviaaugmentationoftheantioxidantsysteminrats AT adewalesegunjames flavonoidrichfractionsfromclerodendrumvolubileandvernoniaamygdalinaextenuatesarsenicinvokedhepatorenaltoxicityviaaugmentationoftheantioxidantsysteminrats AT emmanuelifeanyichukwuugwor flavonoidrichfractionsfromclerodendrumvolubileandvernoniaamygdalinaextenuatesarsenicinvokedhepatorenaltoxicityviaaugmentationoftheantioxidantsysteminrats AT adetolaadedayobabalola flavonoidrichfractionsfromclerodendrumvolubileandvernoniaamygdalinaextenuatesarsenicinvokedhepatorenaltoxicityviaaugmentationoftheantioxidantsysteminrats AT emmanuelobinnaezenandu flavonoidrichfractionsfromclerodendrumvolubileandvernoniaamygdalinaextenuatesarsenicinvokedhepatorenaltoxicityviaaugmentationoftheantioxidantsysteminrats AT victorychukwudaluugbaja flavonoidrichfractionsfromclerodendrumvolubileandvernoniaamygdalinaextenuatesarsenicinvokedhepatorenaltoxicityviaaugmentationoftheantioxidantsysteminrats AT estherayobamiemmanuel flavonoidrichfractionsfromclerodendrumvolubileandvernoniaamygdalinaextenuatesarsenicinvokedhepatorenaltoxicityviaaugmentationoftheantioxidantsysteminrats |