Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2
<i>Streptococcus suis</i> serotype 2 is an important porcine bacterial pathogen associated with multiple pathologies in piglets. Bacterial lipoproteins (LPPs) have been described as playing important roles in the pathogenesis of the infection of other Gram-positive bacteria as adhesins,...
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2386 |
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| author | Servane Payen David Roy Anaïs Boa Masatoshi Okura Jean-Philippe Auger Mariela Segura Marcelo Gottschalk |
| author_facet | Servane Payen David Roy Anaïs Boa Masatoshi Okura Jean-Philippe Auger Mariela Segura Marcelo Gottschalk |
| author_sort | Servane Payen |
| collection | DOAJ |
| description | <i>Streptococcus suis</i> serotype 2 is an important porcine bacterial pathogen associated with multiple pathologies in piglets. Bacterial lipoproteins (LPPs) have been described as playing important roles in the pathogenesis of the infection of other Gram-positive bacteria as adhesins, pro-inflammatory cell activators and/or virulence factors. In the current study, we aimed to evaluate the role of the prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein signal peptidase (Lsp) enzymes, which are responsible for LPP maturation, on the pathogenesis of the infection caused by two different sequence types (STs) of <i>S. suis</i> serotype 2 strains (virulent ST1 and highly virulent ST7). Through the use of isogenic Δ<i>lgt</i>, Δ<i>lsp</i> and double Δ<i>lgt</i>/Δ<i>lsp</i> mutants, it was shown that lack of these enzymes did not influence <i>S. suis</i> adhesion/invasion to porcine respiratory epithelial cells. However, in the absence of the Lsp and/or Lgt, a significant reduction in the capacity of <i>S. suis</i> to activate phagocytic cells and induce pro-inflammatory mediators (in vitro and in vivo) was observed. In general, results obtained with the double mutant did not differ in comparison to single mutants, indicating lack of an additive effect. Finally, our data suggest that these enzymes play a differential role in virulence, depending on the genetic background of the strain and being more important for the highly virulent ST7 strain. |
| first_indexed | 2024-03-10T05:14:38Z |
| format | Article |
| id | doaj.art-460020f45c674a149a2e483a0c8f5007 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:14:38Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-460020f45c674a149a2e483a0c8f50072023-11-23T00:30:41ZengMDPI AGMicroorganisms2076-26072021-11-01911238610.3390/microorganisms9112386Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2Servane Payen0David Roy1Anaïs Boa2Masatoshi Okura3Jean-Philippe Auger4Mariela Segura5Marcelo Gottschalk6Swine and Poultry Infectious Diseases Research Center (CRIPA) and Research Group on Infectious Diseases in Production Animals (GREMIP), Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC J2S 2M2, CanadaSwine and Poultry Infectious Diseases Research Center (CRIPA) and Research Group on Infectious Diseases in Production Animals (GREMIP), Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC J2S 2M2, CanadaSwine and Poultry Infectious Diseases Research Center (CRIPA) and Research Group on Infectious Diseases in Production Animals (GREMIP), Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC J2S 2M2, CanadaDivision of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba 305-0856, JapanSwine and Poultry Infectious Diseases Research Center (CRIPA) and Research Group on Infectious Diseases in Production Animals (GREMIP), Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC J2S 2M2, CanadaSwine and Poultry Infectious Diseases Research Center (CRIPA) and Research Group on Infectious Diseases in Production Animals (GREMIP), Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC J2S 2M2, CanadaSwine and Poultry Infectious Diseases Research Center (CRIPA) and Research Group on Infectious Diseases in Production Animals (GREMIP), Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC J2S 2M2, Canada<i>Streptococcus suis</i> serotype 2 is an important porcine bacterial pathogen associated with multiple pathologies in piglets. Bacterial lipoproteins (LPPs) have been described as playing important roles in the pathogenesis of the infection of other Gram-positive bacteria as adhesins, pro-inflammatory cell activators and/or virulence factors. In the current study, we aimed to evaluate the role of the prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein signal peptidase (Lsp) enzymes, which are responsible for LPP maturation, on the pathogenesis of the infection caused by two different sequence types (STs) of <i>S. suis</i> serotype 2 strains (virulent ST1 and highly virulent ST7). Through the use of isogenic Δ<i>lgt</i>, Δ<i>lsp</i> and double Δ<i>lgt</i>/Δ<i>lsp</i> mutants, it was shown that lack of these enzymes did not influence <i>S. suis</i> adhesion/invasion to porcine respiratory epithelial cells. However, in the absence of the Lsp and/or Lgt, a significant reduction in the capacity of <i>S. suis</i> to activate phagocytic cells and induce pro-inflammatory mediators (in vitro and in vivo) was observed. In general, results obtained with the double mutant did not differ in comparison to single mutants, indicating lack of an additive effect. Finally, our data suggest that these enzymes play a differential role in virulence, depending on the genetic background of the strain and being more important for the highly virulent ST7 strain.https://www.mdpi.com/2076-2607/9/11/2386<i>Streptococcus suis</i>lipoproteinsmaturationinflammation |
| spellingShingle | Servane Payen David Roy Anaïs Boa Masatoshi Okura Jean-Philippe Auger Mariela Segura Marcelo Gottschalk Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2 Microorganisms <i>Streptococcus suis</i> lipoproteins maturation inflammation |
| title | Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2 |
| title_full | Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2 |
| title_fullStr | Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2 |
| title_full_unstemmed | Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2 |
| title_short | Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2 |
| title_sort | role of maturation of lipoproteins in the pathogenesis of the infection caused by i streptococcus suis i serotype 2 |
| topic | <i>Streptococcus suis</i> lipoproteins maturation inflammation |
| url | https://www.mdpi.com/2076-2607/9/11/2386 |
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