Carrageenan from <i>Gigartina skottsbergii</i>: A Novel Molecular Probe to Detect SARS-CoV-2
The COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from <i>Gigartina skottsbergii</i> algae as a probe...
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MDPI AG
2023-03-01
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Series: | Biosensors |
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Online Access: | https://www.mdpi.com/2079-6374/13/3/378 |
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author | Patrícia Daiane Zank Milena Mattes Cerveira Victor Barboza dos Santos Vitor Pereira Klein Thobias Toniolo de Souza Danielle Tapia Bueno Tais Poletti Amanda Fonseca Leitzke Janice Luehring Giongo Neftali Lenin Villarreal Carreño Andrés Mansilla Maria Soledad Astorga-España Claudio Martin Pereira de Pereira Rodrigo de Almeida Vaucher |
author_facet | Patrícia Daiane Zank Milena Mattes Cerveira Victor Barboza dos Santos Vitor Pereira Klein Thobias Toniolo de Souza Danielle Tapia Bueno Tais Poletti Amanda Fonseca Leitzke Janice Luehring Giongo Neftali Lenin Villarreal Carreño Andrés Mansilla Maria Soledad Astorga-España Claudio Martin Pereira de Pereira Rodrigo de Almeida Vaucher |
author_sort | Patrícia Daiane Zank |
collection | DOAJ |
description | The COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from <i>Gigartina skottsbergii</i> algae as a probe for SARS-CoV-2 virus binding capacity and potential use in molecular methods. <i>G. skottsbergii</i> specimens were collected in the Chilean subantarctic ecoregion, and the carrageenan was extracted —using a modified version of Webber’s method—, characterized, and quantified. After 24 h of incubation with an inactivated viral suspension, the carrageenan’s capacity to bind SARS-CoV-2 was tested. The probe-bound viral RNA was quantified using the reverse transcription and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods. Our findings showed that carrageenan extraction from seaweed has a similar spectrum to commercial carrageenan, achieving an excellent proportion of binding to SARS-CoV-2, with a yield of 8.3%. Viral RNA was also detected in the RT-LAMP assay. This study shows, for the first time, the binding capacity of carrageenan extracted from <i>G. skottsbergii</i>, which proved to be a low-cost and highly efficient method of binding to SARS-CoV-2 viral particles. |
first_indexed | 2024-03-11T06:51:26Z |
format | Article |
id | doaj.art-460050b1defc4a08977e1361afa0b10f |
institution | Directory Open Access Journal |
issn | 2079-6374 |
language | English |
last_indexed | 2024-03-11T06:51:26Z |
publishDate | 2023-03-01 |
publisher | MDPI AG |
record_format | Article |
series | Biosensors |
spelling | doaj.art-460050b1defc4a08977e1361afa0b10f2023-11-17T09:54:32ZengMDPI AGBiosensors2079-63742023-03-0113337810.3390/bios13030378Carrageenan from <i>Gigartina skottsbergii</i>: A Novel Molecular Probe to Detect SARS-CoV-2Patrícia Daiane Zank0Milena Mattes Cerveira1Victor Barboza dos Santos2Vitor Pereira Klein3Thobias Toniolo de Souza4Danielle Tapia Bueno5Tais Poletti6Amanda Fonseca Leitzke7Janice Luehring Giongo8Neftali Lenin Villarreal Carreño9Andrés Mansilla10Maria Soledad Astorga-España11Claudio Martin Pereira de Pereira12Rodrigo de Almeida Vaucher13Department of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Laboratory for Lipidomic and Bio-Organic Research, Bioforensic Research Group, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Laboratory for Lipidomic and Bio-Organic Research, Bioforensic Research Group, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Laboratory for Lipidomic and Bio-Organic Research, Bioforensic Research Group, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, BrazilMaterials Science and Engineering Graduate Program, Technology Development Center, Novonano Laboratory, Federal University of Pelotas, Pelotas 96010-610, RS, BrazilAntarctic and Subantarctic Macroalgae Laboratory, Universidad de Magallanes, Punta Arenas 01855, ChileDepartment of Science and Natural Resources, Magallanes Region and Chilean Antarctic, University of Magallanes, Punta Arenas 01855, ChileDepartment of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, BrazilDepartment of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, BrazilThe COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from <i>Gigartina skottsbergii</i> algae as a probe for SARS-CoV-2 virus binding capacity and potential use in molecular methods. <i>G. skottsbergii</i> specimens were collected in the Chilean subantarctic ecoregion, and the carrageenan was extracted —using a modified version of Webber’s method—, characterized, and quantified. After 24 h of incubation with an inactivated viral suspension, the carrageenan’s capacity to bind SARS-CoV-2 was tested. The probe-bound viral RNA was quantified using the reverse transcription and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods. Our findings showed that carrageenan extraction from seaweed has a similar spectrum to commercial carrageenan, achieving an excellent proportion of binding to SARS-CoV-2, with a yield of 8.3%. Viral RNA was also detected in the RT-LAMP assay. This study shows, for the first time, the binding capacity of carrageenan extracted from <i>G. skottsbergii</i>, which proved to be a low-cost and highly efficient method of binding to SARS-CoV-2 viral particles.https://www.mdpi.com/2079-6374/13/3/378COVID-19macroalgacarrageenanprobeqRT-PCRRT-LAMP |
spellingShingle | Patrícia Daiane Zank Milena Mattes Cerveira Victor Barboza dos Santos Vitor Pereira Klein Thobias Toniolo de Souza Danielle Tapia Bueno Tais Poletti Amanda Fonseca Leitzke Janice Luehring Giongo Neftali Lenin Villarreal Carreño Andrés Mansilla Maria Soledad Astorga-España Claudio Martin Pereira de Pereira Rodrigo de Almeida Vaucher Carrageenan from <i>Gigartina skottsbergii</i>: A Novel Molecular Probe to Detect SARS-CoV-2 Biosensors COVID-19 macroalga carrageenan probe qRT-PCR RT-LAMP |
title | Carrageenan from <i>Gigartina skottsbergii</i>: A Novel Molecular Probe to Detect SARS-CoV-2 |
title_full | Carrageenan from <i>Gigartina skottsbergii</i>: A Novel Molecular Probe to Detect SARS-CoV-2 |
title_fullStr | Carrageenan from <i>Gigartina skottsbergii</i>: A Novel Molecular Probe to Detect SARS-CoV-2 |
title_full_unstemmed | Carrageenan from <i>Gigartina skottsbergii</i>: A Novel Molecular Probe to Detect SARS-CoV-2 |
title_short | Carrageenan from <i>Gigartina skottsbergii</i>: A Novel Molecular Probe to Detect SARS-CoV-2 |
title_sort | carrageenan from i gigartina skottsbergii i a novel molecular probe to detect sars cov 2 |
topic | COVID-19 macroalga carrageenan probe qRT-PCR RT-LAMP |
url | https://www.mdpi.com/2079-6374/13/3/378 |
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