Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in Guinea
Abstract Background Pulmonary tuberculosis (PTB) diagnosis relies on sputum examination, a challenge in sputum-scarce patients. Alternative non-invasive sampling methods such as face mask sampling (FMS) have been proposed. Objective To evaluate the value of FMS for PTB diagnosis by assessing its agr...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12941-023-00633-8 |
| _version_ | 1797453602771435520 |
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| author | Souleymane Hassane-Harouna Sofie Marijke Braet Tom Decroo Lansana Mady Camara Alexandre Delamou Sven de Bock Nimer Ortuño-Gutiérrez Gba-Foromo Cherif Caroline M. Williams Anika Wisniewska Michael R. Barer Leen Rigouts Bouke Catherine de Jong |
| author_facet | Souleymane Hassane-Harouna Sofie Marijke Braet Tom Decroo Lansana Mady Camara Alexandre Delamou Sven de Bock Nimer Ortuño-Gutiérrez Gba-Foromo Cherif Caroline M. Williams Anika Wisniewska Michael R. Barer Leen Rigouts Bouke Catherine de Jong |
| author_sort | Souleymane Hassane-Harouna |
| collection | DOAJ |
| description | Abstract Background Pulmonary tuberculosis (PTB) diagnosis relies on sputum examination, a challenge in sputum-scarce patients. Alternative non-invasive sampling methods such as face mask sampling (FMS) have been proposed. Objective To evaluate the value of FMS for PTB diagnosis by assessing its agreement with sputum samples processed by GeneXpert MTB/RIF (Ultra)(Xpert) testing, and describe FMS sensitivity and specificity. Methods This was a prospective study conducted at the Carrière TB clinic in Guinea. Presumptive TB patients willing to participate were asked to wear a surgical mask containing a polyvinyl alcohol (PVA) strip for thirty minutes. Subsequently, two spot sputum samples were collected, of which one was processed by microscopy on site and the other by Xpert in Guinea’s National Reference Laboratory of Mycobacteriology (LNRM). The first 30 FMS were processed at the Supranational Reference Laboratory in Antwerp, Belgium, and the following 118 FMS in the LNRM. Results One hundred fifty patients participated, of whom 148 had valid results for both mask and sputum. Sputum smear microscopy was positive for 47 (31.8%) patients while sputum-Xpert detected MTB in 54 (36.5%) patients. Among the 54 patients testing sputum-Xpert positive, 26 (48.1%) yielded a positive FMS-Xpert result, while four sputum-Xpert negative patients tested positive for FMS and 90 patients were Xpert-negative for both sputum and mask samples, suggesting a moderate level of agreement (k-value of 0.47). The overall mask sensitivity was 48.1%, with 95.7% specificity. Conclusion In our setting, Xpert testing on FMS did not yield a high level of agreement to sputum sample. |
| first_indexed | 2024-03-09T15:25:12Z |
| format | Article |
| id | doaj.art-46013034faed499aa1c263c8e6e05325 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-03-09T15:25:12Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-46013034faed499aa1c263c8e6e053252023-11-26T12:34:47ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112023-09-012211710.1186/s12941-023-00633-8Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in GuineaSouleymane Hassane-Harouna0Sofie Marijke Braet1Tom Decroo2Lansana Mady Camara3Alexandre Delamou4Sven de Bock5Nimer Ortuño-Gutiérrez6Gba-Foromo Cherif7Caroline M. Williams8Anika Wisniewska9Michael R. Barer10Leen Rigouts11Bouke Catherine de Jong12Action Damien ConakryResearch Foundation FlandersUnit of HIV & Co-infections, Department of Clinical Sciences, Institute of Tropical MedicineGamal Abdel Nasser UniversityGamal Abdel Nasser UniversityUnit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical MedicineDamien FoundationAction Damien ConakryDepartment of Infection, Immunity and Inflammation, University of LeicesterDepartment of Infection, Immunity and Inflammation, University of LeicesterDepartment of Infection, Immunity and Inflammation, University of LeicesterUnit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical MedicineUnit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical MedicineAbstract Background Pulmonary tuberculosis (PTB) diagnosis relies on sputum examination, a challenge in sputum-scarce patients. Alternative non-invasive sampling methods such as face mask sampling (FMS) have been proposed. Objective To evaluate the value of FMS for PTB diagnosis by assessing its agreement with sputum samples processed by GeneXpert MTB/RIF (Ultra)(Xpert) testing, and describe FMS sensitivity and specificity. Methods This was a prospective study conducted at the Carrière TB clinic in Guinea. Presumptive TB patients willing to participate were asked to wear a surgical mask containing a polyvinyl alcohol (PVA) strip for thirty minutes. Subsequently, two spot sputum samples were collected, of which one was processed by microscopy on site and the other by Xpert in Guinea’s National Reference Laboratory of Mycobacteriology (LNRM). The first 30 FMS were processed at the Supranational Reference Laboratory in Antwerp, Belgium, and the following 118 FMS in the LNRM. Results One hundred fifty patients participated, of whom 148 had valid results for both mask and sputum. Sputum smear microscopy was positive for 47 (31.8%) patients while sputum-Xpert detected MTB in 54 (36.5%) patients. Among the 54 patients testing sputum-Xpert positive, 26 (48.1%) yielded a positive FMS-Xpert result, while four sputum-Xpert negative patients tested positive for FMS and 90 patients were Xpert-negative for both sputum and mask samples, suggesting a moderate level of agreement (k-value of 0.47). The overall mask sensitivity was 48.1%, with 95.7% specificity. Conclusion In our setting, Xpert testing on FMS did not yield a high level of agreement to sputum sample.https://doi.org/10.1186/s12941-023-00633-8Face mask samplingPulmonary tuberculosisGuinea |
| spellingShingle | Souleymane Hassane-Harouna Sofie Marijke Braet Tom Decroo Lansana Mady Camara Alexandre Delamou Sven de Bock Nimer Ortuño-Gutiérrez Gba-Foromo Cherif Caroline M. Williams Anika Wisniewska Michael R. Barer Leen Rigouts Bouke Catherine de Jong Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in Guinea Annals of Clinical Microbiology and Antimicrobials Face mask sampling Pulmonary tuberculosis Guinea |
| title | Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in Guinea |
| title_full | Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in Guinea |
| title_fullStr | Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in Guinea |
| title_full_unstemmed | Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in Guinea |
| title_short | Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in Guinea |
| title_sort | face mask sampling fms for tuberculosis shows lower diagnostic sensitivity than sputum sampling in guinea |
| topic | Face mask sampling Pulmonary tuberculosis Guinea |
| url | https://doi.org/10.1186/s12941-023-00633-8 |
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