Dysfunctional Pro1 leads to female sterility in rice blast fungi
Summary: Although sexual reproduction is widespread in eukaryotes, some fungal species can only reproduce asexually. In the rice blast fungus Pyricularia (Magnaporthe) oryzae, several isolates from the region of origin retain mating ability, but most isolates are female sterile. Therefore, female fe...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223010970 |
| _version_ | 1797774540289343488 |
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| author | Momotaka Uchida Takahiro Konishi Ayaka Fujigasaki Kohtetsu Kita Tsutomu Arie Tohru Teraoka Yasukazu Kanda Masaki Mori Takayuki Arazoe Takashi Kamakura |
| author_facet | Momotaka Uchida Takahiro Konishi Ayaka Fujigasaki Kohtetsu Kita Tsutomu Arie Tohru Teraoka Yasukazu Kanda Masaki Mori Takayuki Arazoe Takashi Kamakura |
| author_sort | Momotaka Uchida |
| collection | DOAJ |
| description | Summary: Although sexual reproduction is widespread in eukaryotes, some fungal species can only reproduce asexually. In the rice blast fungus Pyricularia (Magnaporthe) oryzae, several isolates from the region of origin retain mating ability, but most isolates are female sterile. Therefore, female fertility may have been lost during its spread from the origin. Here, we show that functional mutations of Pro1, a global transcriptional regulator of mating-related genes in filamentous fungi, is one cause of loss of female fertility in this fungus. We identified the mutation of Pro1 by backcrossing analysis between female-fertile and female-sterile isolates. The dysfunctional Pro1 did not affect the infection processes but conidial release was increased. Furthermore, various mutations in Pro1 were detected in geographically distant P. oryzae, including pandemic isolates of wheat blast fungus. These results provide the first evidence that loss of female fertility may be advantageous to the life cycle of some plant pathogenic fungi. |
| first_indexed | 2024-03-12T22:22:33Z |
| format | Article |
| id | doaj.art-4601b3a277904a86aa16101a396570ca |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:22:33Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-4601b3a277904a86aa16101a396570ca2023-07-23T04:55:11ZengElsevieriScience2589-00422023-07-01267107020Dysfunctional Pro1 leads to female sterility in rice blast fungiMomotaka Uchida0Takahiro Konishi1Ayaka Fujigasaki2Kohtetsu Kita3Tsutomu Arie4Tohru Teraoka5Yasukazu Kanda6Masaki Mori7Takayuki Arazoe8Takashi Kamakura9Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, JapanDepartment of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, JapanDepartment of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, JapanDepartment of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, JapanUnited Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-0054, JapanUnited Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-0054, JapanDivision of Plant Molecular Regulation Research, Institute of Agrobiological Sciences, NARO (NIAS), 2-1-2 Kan-nondai, Tsukuba, Ibaraki 305-8602, JapanDivision of Plant Molecular Regulation Research, Institute of Agrobiological Sciences, NARO (NIAS), 2-1-2 Kan-nondai, Tsukuba, Ibaraki 305-8602, JapanDepartment of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, JapanDepartment of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Corresponding authorSummary: Although sexual reproduction is widespread in eukaryotes, some fungal species can only reproduce asexually. In the rice blast fungus Pyricularia (Magnaporthe) oryzae, several isolates from the region of origin retain mating ability, but most isolates are female sterile. Therefore, female fertility may have been lost during its spread from the origin. Here, we show that functional mutations of Pro1, a global transcriptional regulator of mating-related genes in filamentous fungi, is one cause of loss of female fertility in this fungus. We identified the mutation of Pro1 by backcrossing analysis between female-fertile and female-sterile isolates. The dysfunctional Pro1 did not affect the infection processes but conidial release was increased. Furthermore, various mutations in Pro1 were detected in geographically distant P. oryzae, including pandemic isolates of wheat blast fungus. These results provide the first evidence that loss of female fertility may be advantageous to the life cycle of some plant pathogenic fungi.http://www.sciencedirect.com/science/article/pii/S2589004223010970MicrobiologyEvolutionary biologyPlant pathology |
| spellingShingle | Momotaka Uchida Takahiro Konishi Ayaka Fujigasaki Kohtetsu Kita Tsutomu Arie Tohru Teraoka Yasukazu Kanda Masaki Mori Takayuki Arazoe Takashi Kamakura Dysfunctional Pro1 leads to female sterility in rice blast fungi iScience Microbiology Evolutionary biology Plant pathology |
| title | Dysfunctional Pro1 leads to female sterility in rice blast fungi |
| title_full | Dysfunctional Pro1 leads to female sterility in rice blast fungi |
| title_fullStr | Dysfunctional Pro1 leads to female sterility in rice blast fungi |
| title_full_unstemmed | Dysfunctional Pro1 leads to female sterility in rice blast fungi |
| title_short | Dysfunctional Pro1 leads to female sterility in rice blast fungi |
| title_sort | dysfunctional pro1 leads to female sterility in rice blast fungi |
| topic | Microbiology Evolutionary biology Plant pathology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223010970 |
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