Comprehensive analysis of the prognostic value and immunological role of IDO1 gene in pan-cancer
Abstract Objective It has been demonstrated that IDO1, a target of immune checkpoint inhibition, functions as an oncogene in the majority of human malignancies. IDO1’s function in human pan-cancers hasn’t been thoroughly studied, though. Materials and methods The Kaplan–Meier (K-M) and COX analyses...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-024-01766-y |
| _version_ | 1797233738201956352 |
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| author | Kaili Lin Yongfeng Wang Fangyu Liu Taifu You Xiongxiong Liu Runzhang Liu Zeyang Li Chunyu Zhen Yunxia Zhang Xingguang Liu Hui Cai |
| author_facet | Kaili Lin Yongfeng Wang Fangyu Liu Taifu You Xiongxiong Liu Runzhang Liu Zeyang Li Chunyu Zhen Yunxia Zhang Xingguang Liu Hui Cai |
| author_sort | Kaili Lin |
| collection | DOAJ |
| description | Abstract Objective It has been demonstrated that IDO1, a target of immune checkpoint inhibition, functions as an oncogene in the majority of human malignancies. IDO1’s function in human pan-cancers hasn’t been thoroughly studied, though. Materials and methods The Kaplan–Meier (K-M) and COX analyses were applied to the survival analysis. Furthermore, we used Spearman’s correlation analysis to examine the associations between IDO1 and microsatellite instability (MSI), DNA methyltransferases (DNMTs), tumor mutational burden (TMB), the associated genes of mismatch repair (MMR), and immune checkpoint biomarkers. Moreover, immunohistochemical analysis and qRT-PCR were used to evaluate IDO1’s expression in pan-cancer cells. Results The findings of this study reveal that IDO1 has abnormal expression in a number of malignancies and is related to the prognosis for UVM, LGG, KIRP, GBM, LAML, OV, READ, MESO, SARC, SKCM, and HNSC. Furthermore, the aberrant IDO1 expression was connected to the TMB, MSI, MMR, drug sensitivity, immune cells infiltrating, and tumor immune microenvironment across a variety of cancer types. The PCR results showed that in contrast to normal cells, IDO1 was found to be significantly highly expressed in breast cancer cells and hepatocellular carcinoma cells, and significantly lowly expressed in gastric cancer cells. Conclusion The clinical treatment of IDO1 is now better supported by a theoretical basis and guidelines provided by our study. |
| first_indexed | 2024-04-24T16:20:56Z |
| format | Article |
| id | doaj.art-4601ee29489649558ea1996db53105b7 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-24T16:20:56Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-4601ee29489649558ea1996db53105b72024-03-31T11:13:42ZengBMCEuropean Journal of Medical Research2047-783X2024-03-0129111910.1186/s40001-024-01766-yComprehensive analysis of the prognostic value and immunological role of IDO1 gene in pan-cancerKaili Lin0Yongfeng Wang1Fangyu Liu2Taifu You3Xiongxiong Liu4Runzhang Liu5Zeyang Li6Chunyu Zhen7Yunxia Zhang8Xingguang Liu9Hui Cai10Lanzhou First People’s HospitalThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityGeneral Surgery Clinical Medical Center, Gansu Provincial HospitalAbstract Objective It has been demonstrated that IDO1, a target of immune checkpoint inhibition, functions as an oncogene in the majority of human malignancies. IDO1’s function in human pan-cancers hasn’t been thoroughly studied, though. Materials and methods The Kaplan–Meier (K-M) and COX analyses were applied to the survival analysis. Furthermore, we used Spearman’s correlation analysis to examine the associations between IDO1 and microsatellite instability (MSI), DNA methyltransferases (DNMTs), tumor mutational burden (TMB), the associated genes of mismatch repair (MMR), and immune checkpoint biomarkers. Moreover, immunohistochemical analysis and qRT-PCR were used to evaluate IDO1’s expression in pan-cancer cells. Results The findings of this study reveal that IDO1 has abnormal expression in a number of malignancies and is related to the prognosis for UVM, LGG, KIRP, GBM, LAML, OV, READ, MESO, SARC, SKCM, and HNSC. Furthermore, the aberrant IDO1 expression was connected to the TMB, MSI, MMR, drug sensitivity, immune cells infiltrating, and tumor immune microenvironment across a variety of cancer types. The PCR results showed that in contrast to normal cells, IDO1 was found to be significantly highly expressed in breast cancer cells and hepatocellular carcinoma cells, and significantly lowly expressed in gastric cancer cells. Conclusion The clinical treatment of IDO1 is now better supported by a theoretical basis and guidelines provided by our study.https://doi.org/10.1186/s40001-024-01766-yIDO1PrognosisBioinformatics analysisCancers |
| spellingShingle | Kaili Lin Yongfeng Wang Fangyu Liu Taifu You Xiongxiong Liu Runzhang Liu Zeyang Li Chunyu Zhen Yunxia Zhang Xingguang Liu Hui Cai Comprehensive analysis of the prognostic value and immunological role of IDO1 gene in pan-cancer European Journal of Medical Research IDO1 Prognosis Bioinformatics analysis Cancers |
| title | Comprehensive analysis of the prognostic value and immunological role of IDO1 gene in pan-cancer |
| title_full | Comprehensive analysis of the prognostic value and immunological role of IDO1 gene in pan-cancer |
| title_fullStr | Comprehensive analysis of the prognostic value and immunological role of IDO1 gene in pan-cancer |
| title_full_unstemmed | Comprehensive analysis of the prognostic value and immunological role of IDO1 gene in pan-cancer |
| title_short | Comprehensive analysis of the prognostic value and immunological role of IDO1 gene in pan-cancer |
| title_sort | comprehensive analysis of the prognostic value and immunological role of ido1 gene in pan cancer |
| topic | IDO1 Prognosis Bioinformatics analysis Cancers |
| url | https://doi.org/10.1186/s40001-024-01766-y |
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