Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
Cytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/66393 |
| _version_ | 1797748550971424768 |
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| author | Meredith H Wilson Stephen C Ekker Steven A Farber |
| author_facet | Meredith H Wilson Stephen C Ekker Steven A Farber |
| author_sort | Meredith H Wilson |
| collection | DOAJ |
| description | Cytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful transgenic and in vivo imaging opportunities available in zebrafish, we built a suite of tools to study lipid droplets in real time from the subcellular to the whole organism level. Fluorescently tagging the lipid droplet-associated proteins, perilipin 2 and perilipin 3, in the endogenous loci permits visualization of lipid droplets in the intestine, liver, and adipose tissue. Using these tools, we found that perilipin 3 is rapidly loaded on intestinal lipid droplets following a high-fat meal and later replaced by perilipin 2. These powerful new tools will facilitate studies on the role of lipid droplets in different tissues, under different genetic and physiological manipulations, and in a variety of human disease models. |
| first_indexed | 2024-03-12T16:06:25Z |
| format | Article |
| id | doaj.art-46058fdd397a40a9828b3b8699040bfe |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-12T16:06:25Z |
| publishDate | 2021-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-46058fdd397a40a9828b3b8699040bfe2023-08-09T14:19:48ZengeLife Sciences Publications LtdeLife2050-084X2021-08-011010.7554/eLife.66393Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafishMeredith H Wilson0https://orcid.org/0000-0002-6152-7127Stephen C Ekker1https://orcid.org/0000-0003-0726-4212Steven A Farber2https://orcid.org/0000-0002-8037-7312Carnegie Institution for Science Department of Embryology, Baltimore, United StatesDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United StatesCarnegie Institution for Science Department of Embryology, Baltimore, United States; Johns Hopkins University Department of Biology, Baltimore, United StatesCytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful transgenic and in vivo imaging opportunities available in zebrafish, we built a suite of tools to study lipid droplets in real time from the subcellular to the whole organism level. Fluorescently tagging the lipid droplet-associated proteins, perilipin 2 and perilipin 3, in the endogenous loci permits visualization of lipid droplets in the intestine, liver, and adipose tissue. Using these tools, we found that perilipin 3 is rapidly loaded on intestinal lipid droplets following a high-fat meal and later replaced by perilipin 2. These powerful new tools will facilitate studies on the role of lipid droplets in different tissues, under different genetic and physiological manipulations, and in a variety of human disease models.https://elifesciences.org/articles/66393lipid dropletperilipinPLIN |
| spellingShingle | Meredith H Wilson Stephen C Ekker Steven A Farber Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish eLife lipid droplet perilipin PLIN |
| title | Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish |
| title_full | Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish |
| title_fullStr | Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish |
| title_full_unstemmed | Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish |
| title_short | Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish |
| title_sort | imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock in zebrafish |
| topic | lipid droplet perilipin PLIN |
| url | https://elifesciences.org/articles/66393 |
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