Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells
Infant biscuits (IBs) are part of complementary feeding from weaning up to the age of five years. They normally contain bovine milk proteins, which can influence bone development. This potential effect was investigated using experimental baked IBs, which were prepared from doughs containing differen...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/9/10/1510 |
| _version_ | 1797550307050258432 |
|---|---|
| author | Michela Bottani Stefano Cattaneo Valentina Pica Milda Stuknytė Marta Gomarasca Giovanni Lombardi Giuseppe Banfi Ivano De Noni Anita Ferraretto |
| author_facet | Michela Bottani Stefano Cattaneo Valentina Pica Milda Stuknytė Marta Gomarasca Giovanni Lombardi Giuseppe Banfi Ivano De Noni Anita Ferraretto |
| author_sort | Michela Bottani |
| collection | DOAJ |
| description | Infant biscuits (IBs) are part of complementary feeding from weaning up to the age of five years. They normally contain bovine milk proteins, which can influence bone development. This potential effect was investigated using experimental baked IBs, which were prepared from doughs containing different type of dairy proteins: milk protein concentrate (IB1), whey protein isolate (IB2), and skimmed milk powder (IB3). Dairy protein-free (IB0) and gluten-free (IB4) biscuits were also formulated. The in vitro gastrointestinal digests of IBs (IBDs) were tested on a co-culture of Caco-2/HT-29 70/30 cells as an in vitro model of human small intestine. None of the IBDs influenced cell viability and monolayer integrity, while IBD0 and IBD4 increased Peptide-YY production. The basolateral contents of Transwell plates seeded with Caco-2/HT-29 70/30 co-culture, mimicking metabolized IBDs (MIBDs), were tested on Saos-2 cells, an in vitro model of human osteoblast-like cells. After incubation, MIBD0, lacking dairy proteins, decreased the cell viability, while MIBD2, containing whey protein isolate, increased both the viability and the number of cells. MIBD2 and MIBD4, the latter containing both casein and whey proteins, increased alkaline phosphatase activity, a bone differentiation marker. These results highlight that IBs containing dairy proteins positively affect bone development. |
| first_indexed | 2024-03-10T15:27:26Z |
| format | Article |
| id | doaj.art-4606830b525b4762893be9024206726a |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T15:27:26Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-4606830b525b4762893be9024206726a2023-11-20T17:53:50ZengMDPI AGFoods2304-81582020-10-01910151010.3390/foods9101510Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like CellsMichela Bottani0Stefano Cattaneo1Valentina Pica2Milda Stuknytė3Marta Gomarasca4Giovanni Lombardi5Giuseppe Banfi6Ivano De Noni7Anita Ferraretto8Laboratory of Experimental Biochemistry & Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Via Galeazzi 4, 20161 Milan, ItalyDepartment of Food, Environmental and Nutritional Sciences, University of Milan, Via Celoria 2, 20133 Milan, ItalyDepartment of Food, Environmental and Nutritional Sciences, University of Milan, Via Celoria 2, 20133 Milan, ItalyUnitech COSPECT—University Technological Platforms Office, University of Milan, Via Golgi 19, 20133 Milan, ItalyLaboratory of Experimental Biochemistry & Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Via Galeazzi 4, 20161 Milan, ItalyLaboratory of Experimental Biochemistry & Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Via Galeazzi 4, 20161 Milan, ItalyLaboratory of Experimental Biochemistry & Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Via Galeazzi 4, 20161 Milan, ItalyDepartment of Food, Environmental and Nutritional Sciences, University of Milan, Via Celoria 2, 20133 Milan, ItalyLaboratory of Experimental Biochemistry & Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Via Galeazzi 4, 20161 Milan, ItalyInfant biscuits (IBs) are part of complementary feeding from weaning up to the age of five years. They normally contain bovine milk proteins, which can influence bone development. This potential effect was investigated using experimental baked IBs, which were prepared from doughs containing different type of dairy proteins: milk protein concentrate (IB1), whey protein isolate (IB2), and skimmed milk powder (IB3). Dairy protein-free (IB0) and gluten-free (IB4) biscuits were also formulated. The in vitro gastrointestinal digests of IBs (IBDs) were tested on a co-culture of Caco-2/HT-29 70/30 cells as an in vitro model of human small intestine. None of the IBDs influenced cell viability and monolayer integrity, while IBD0 and IBD4 increased Peptide-YY production. The basolateral contents of Transwell plates seeded with Caco-2/HT-29 70/30 co-culture, mimicking metabolized IBDs (MIBDs), were tested on Saos-2 cells, an in vitro model of human osteoblast-like cells. After incubation, MIBD0, lacking dairy proteins, decreased the cell viability, while MIBD2, containing whey protein isolate, increased both the viability and the number of cells. MIBD2 and MIBD4, the latter containing both casein and whey proteins, increased alkaline phosphatase activity, a bone differentiation marker. These results highlight that IBs containing dairy proteins positively affect bone development.https://www.mdpi.com/2304-8158/9/10/1510infant biscuitsdairy proteinswheycaseinCaco-2/HT-29 co-culture cellsSaos-2 cells |
| spellingShingle | Michela Bottani Stefano Cattaneo Valentina Pica Milda Stuknytė Marta Gomarasca Giovanni Lombardi Giuseppe Banfi Ivano De Noni Anita Ferraretto Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells Foods infant biscuits dairy proteins whey casein Caco-2/HT-29 co-culture cells Saos-2 cells |
| title | Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells |
| title_full | Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells |
| title_fullStr | Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells |
| title_full_unstemmed | Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells |
| title_short | Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells |
| title_sort | gastrointestinal in vitro digests of infant biscuits formulated with bovine milk proteins positively affect in vitro differentiation of human osteoblast like cells |
| topic | infant biscuits dairy proteins whey casein Caco-2/HT-29 co-culture cells Saos-2 cells |
| url | https://www.mdpi.com/2304-8158/9/10/1510 |
| work_keys_str_mv | AT michelabottani gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells AT stefanocattaneo gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells AT valentinapica gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells AT mildastuknyte gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells AT martagomarasca gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells AT giovannilombardi gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells AT giuseppebanfi gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells AT ivanodenoni gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells AT anitaferraretto gastrointestinalinvitrodigestsofinfantbiscuitsformulatedwithbovinemilkproteinspositivelyaffectinvitrodifferentiationofhumanosteoblastlikecells |