Bridging potential of Taurine-loading PCL conduits transplanted with hEnSCs on resected sciatic nerves
Reconstruction of nerve conduits is a promising method for functional improvement in peripheral nerve repair. Besides choosing of a suitable polymer for conduit construction, adding factors such as Taurine improve a more advantageous microenvironment for defect nerve regeneration. Showing several ma...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320422000906 |
| _version_ | 1811188629251293184 |
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| author | Arman Ai Jamileh Saremi Somayeh Ebrahimi-Barough Narges Fereydouni Tara Mahmoodi Nastaran Kazemi rad Pedram Sarikhani Arash goodarzi Fardin Amidi |
| author_facet | Arman Ai Jamileh Saremi Somayeh Ebrahimi-Barough Narges Fereydouni Tara Mahmoodi Nastaran Kazemi rad Pedram Sarikhani Arash goodarzi Fardin Amidi |
| author_sort | Arman Ai |
| collection | DOAJ |
| description | Reconstruction of nerve conduits is a promising method for functional improvement in peripheral nerve repair. Besides choosing of a suitable polymer for conduit construction, adding factors such as Taurine improve a more advantageous microenvironment for defect nerve regeneration. Showing several major biological properties of Taurine, for example, regulation of the osmotic pressure, modulation of neurogenesis, and calcium hemostasis, makes it an appropriate option for repairing of defected nerves. To this, we examined repairing effects of Taurine-loading PCL conduits cultured with human endothelial stem cells (hEnSCs) on resected sciatic nerves. PCL/Taurine/Cell conduits transplanted to a 10-mm sciatic nerve gap. Forty-two wistar rats were randomly divided to seven groups: (1) Normal group, (2) Negative control (NC), (3) Positive control (nerve Autograft group), (4) PCL conduits group (PCL), (5) Taurine loaded PCL conduits group (PCL/Taurine), (6) hEnSCs cultured on the PCL conduits (PCL/Cell), (7) hEnSCs cultured on the PCL/Taurine conduits (PCL/Taurine/Cell). Functional recovery of motor and sensory nerves, the action potential of exciting muscle and motor distal latency has seen in PCL/Taurine/Cell conduits. Histological studies showed also remarkable nerve regeneration and obvious bridging has seen in this group. In conclusion, PCL/Taurine/Cell conduits showing suitable mechanical properties and biocompatibility may improve sciatic nerve regeneration. |
| first_indexed | 2024-04-11T14:23:12Z |
| format | Article |
| id | doaj.art-4606f386105c4fb6b241382aacc5fcd4 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-04-11T14:23:12Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-4606f386105c4fb6b241382aacc5fcd42022-12-22T04:19:00ZengElsevierRegenerative Therapy2352-32042022-12-0121424435Bridging potential of Taurine-loading PCL conduits transplanted with hEnSCs on resected sciatic nervesArman Ai0Jamileh Saremi1Somayeh Ebrahimi-Barough2Narges Fereydouni3Tara Mahmoodi4Nastaran Kazemi rad5Pedram Sarikhani6 Arash goodarzi7Fardin Amidi8School of Medicine, Tehran University of Medical Sciences, Tehran, IranResearch Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Tissue engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran; Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, IranSchool of Medicine, Tehran University of Medical Sciences, Tehran, IranSchool of Medicine, Tehran University of Medical Sciences, Tehran, IranSchool of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Tissue engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran; Corresponding author. Department of Tissue engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, IranDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Corresponding author. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, IranReconstruction of nerve conduits is a promising method for functional improvement in peripheral nerve repair. Besides choosing of a suitable polymer for conduit construction, adding factors such as Taurine improve a more advantageous microenvironment for defect nerve regeneration. Showing several major biological properties of Taurine, for example, regulation of the osmotic pressure, modulation of neurogenesis, and calcium hemostasis, makes it an appropriate option for repairing of defected nerves. To this, we examined repairing effects of Taurine-loading PCL conduits cultured with human endothelial stem cells (hEnSCs) on resected sciatic nerves. PCL/Taurine/Cell conduits transplanted to a 10-mm sciatic nerve gap. Forty-two wistar rats were randomly divided to seven groups: (1) Normal group, (2) Negative control (NC), (3) Positive control (nerve Autograft group), (4) PCL conduits group (PCL), (5) Taurine loaded PCL conduits group (PCL/Taurine), (6) hEnSCs cultured on the PCL conduits (PCL/Cell), (7) hEnSCs cultured on the PCL/Taurine conduits (PCL/Taurine/Cell). Functional recovery of motor and sensory nerves, the action potential of exciting muscle and motor distal latency has seen in PCL/Taurine/Cell conduits. Histological studies showed also remarkable nerve regeneration and obvious bridging has seen in this group. In conclusion, PCL/Taurine/Cell conduits showing suitable mechanical properties and biocompatibility may improve sciatic nerve regeneration.http://www.sciencedirect.com/science/article/pii/S2352320422000906Nerve regenerationTaurineHuman endothelial stem cells (hEnSCs) |
| spellingShingle | Arman Ai Jamileh Saremi Somayeh Ebrahimi-Barough Narges Fereydouni Tara Mahmoodi Nastaran Kazemi rad Pedram Sarikhani Arash goodarzi Fardin Amidi Bridging potential of Taurine-loading PCL conduits transplanted with hEnSCs on resected sciatic nerves Regenerative Therapy Nerve regeneration Taurine Human endothelial stem cells (hEnSCs) |
| title | Bridging potential of Taurine-loading PCL conduits transplanted with hEnSCs on resected sciatic nerves |
| title_full | Bridging potential of Taurine-loading PCL conduits transplanted with hEnSCs on resected sciatic nerves |
| title_fullStr | Bridging potential of Taurine-loading PCL conduits transplanted with hEnSCs on resected sciatic nerves |
| title_full_unstemmed | Bridging potential of Taurine-loading PCL conduits transplanted with hEnSCs on resected sciatic nerves |
| title_short | Bridging potential of Taurine-loading PCL conduits transplanted with hEnSCs on resected sciatic nerves |
| title_sort | bridging potential of taurine loading pcl conduits transplanted with henscs on resected sciatic nerves |
| topic | Nerve regeneration Taurine Human endothelial stem cells (hEnSCs) |
| url | http://www.sciencedirect.com/science/article/pii/S2352320422000906 |
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