Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system
While there has been progress in detection of drug resistant tuberculosis globally, WHO estimates only about half of the patients with bacteriologically confirmed tuberculosis were tested for rifampicin resistance over the past two years. To close this drug resistance diagnostic gap, an expansion of...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Journal of Clinical Tuberculosis and Other Mycobacterial Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405579422000092 |
| _version_ | 1818242463191531520 |
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| author | Rebecca E. Colman Christopher Hagan Peter Chiles Marva Seifert Donald G. Catanzaro Alexander V. Kukhtin Ryan Norville Laura Hauns Arial Bueno Rebecca C. Holmberg Christopher G. Cooney Timothy C. Rodwell |
| author_facet | Rebecca E. Colman Christopher Hagan Peter Chiles Marva Seifert Donald G. Catanzaro Alexander V. Kukhtin Ryan Norville Laura Hauns Arial Bueno Rebecca C. Holmberg Christopher G. Cooney Timothy C. Rodwell |
| author_sort | Rebecca E. Colman |
| collection | DOAJ |
| description | While there has been progress in detection of drug resistant tuberculosis globally, WHO estimates only about half of the patients with bacteriologically confirmed tuberculosis were tested for rifampicin resistance over the past two years. To close this drug resistance diagnostic gap, an expansion of testing for rifampicin and isoniazid resistance is critically needed. The Akonni Biosystem Integrated System combines DNA extraction and a Lab-on-a-Film assembly (LFA) to perform rapid probe and PCR-based detection of resistance associated mutations to first-line anti-tuberculosis drugs. Using raw sputum samples from 25 tuberculosis patients at risk for drug resistance, we conducted a proof-of-concept study of the Integrated System with an MDR-TB assay. Performance of the Integrated System was compared to liquid Mycobacteria Growth Indicator Tube (MGIT) culture reference phenotypes using 2012 WHO endorsed critical concentrations for rifampicin and isoniazid. The overall percent agreement for rifampicin and isoniazid was 91.7% and 100% respectively, with agreement for rifampicin increasing to 95.7% after low-level resistance mutations in rpoB were excluded. The Integrated System, combining DNA extraction and LFA amplification, is a promising new tool for detection of both rifampicin and isoniazid using liquefied raw sputum. |
| first_indexed | 2024-12-12T13:45:38Z |
| format | Article |
| id | doaj.art-4609f1900a1b442aaa7aa0e9ac557218 |
| institution | Directory Open Access Journal |
| issn | 2405-5794 |
| language | English |
| last_indexed | 2024-12-12T13:45:38Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Clinical Tuberculosis and Other Mycobacterial Diseases |
| spelling | doaj.art-4609f1900a1b442aaa7aa0e9ac5572182022-12-22T00:22:41ZengElsevierJournal of Clinical Tuberculosis and Other Mycobacterial Diseases2405-57942022-05-0127100304Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated systemRebecca E. Colman0Christopher Hagan1Peter Chiles2Marva Seifert3Donald G. Catanzaro4Alexander V. Kukhtin5Ryan Norville6Laura Hauns7Arial Bueno8Rebecca C. Holmberg9Christopher G. Cooney10Timothy C. Rodwell11Department of Medicine, University of California, San Diego, La Jolla, CA, USA; Corresponding author.Department of Medicine, University of California, San Diego, La Jolla, CA, USADepartment of Medicine, University of California, San Diego, La Jolla, CA, USADepartment of Medicine, University of California, San Diego, La Jolla, CA, USADepartment of Biological Sciences, University of Arkansas, Fayetteville, AR, USAAkonni Biosystems, Inc., Frederick, MD, USAAkonni Biosystems, Inc., Frederick, MD, USAAkonni Biosystems, Inc., Frederick, MD, USAAkonni Biosystems, Inc., Frederick, MD, USAAkonni Biosystems, Inc., Frederick, MD, USAAkonni Biosystems, Inc., Frederick, MD, USADepartment of Medicine, University of California, San Diego, La Jolla, CA, USAWhile there has been progress in detection of drug resistant tuberculosis globally, WHO estimates only about half of the patients with bacteriologically confirmed tuberculosis were tested for rifampicin resistance over the past two years. To close this drug resistance diagnostic gap, an expansion of testing for rifampicin and isoniazid resistance is critically needed. The Akonni Biosystem Integrated System combines DNA extraction and a Lab-on-a-Film assembly (LFA) to perform rapid probe and PCR-based detection of resistance associated mutations to first-line anti-tuberculosis drugs. Using raw sputum samples from 25 tuberculosis patients at risk for drug resistance, we conducted a proof-of-concept study of the Integrated System with an MDR-TB assay. Performance of the Integrated System was compared to liquid Mycobacteria Growth Indicator Tube (MGIT) culture reference phenotypes using 2012 WHO endorsed critical concentrations for rifampicin and isoniazid. The overall percent agreement for rifampicin and isoniazid was 91.7% and 100% respectively, with agreement for rifampicin increasing to 95.7% after low-level resistance mutations in rpoB were excluded. The Integrated System, combining DNA extraction and LFA amplification, is a promising new tool for detection of both rifampicin and isoniazid using liquefied raw sputum.http://www.sciencedirect.com/science/article/pii/S2405579422000092Multidrug-resistant tuberculosis diagnosticBenchtop automated system |
| spellingShingle | Rebecca E. Colman Christopher Hagan Peter Chiles Marva Seifert Donald G. Catanzaro Alexander V. Kukhtin Ryan Norville Laura Hauns Arial Bueno Rebecca C. Holmberg Christopher G. Cooney Timothy C. Rodwell Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Multidrug-resistant tuberculosis diagnostic Benchtop automated system |
| title | Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system |
| title_full | Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system |
| title_fullStr | Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system |
| title_full_unstemmed | Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system |
| title_short | Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system |
| title_sort | detecting rifampin and isoniazid resistance in mycobacterium tuberculosis direct from patient sputum using an automated integrated system |
| topic | Multidrug-resistant tuberculosis diagnostic Benchtop automated system |
| url | http://www.sciencedirect.com/science/article/pii/S2405579422000092 |
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