Antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran
Background and Objective: Water resident bacteria are potentially important reservoir of antibiotic resistance genes. This study was performed to evaluate the antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran. Methods: In this descriptive...
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| Format: | Article |
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Golestan University of Medical Sciences
2016-12-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
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| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-952&slc_lang=en&sid=1 |
| _version_ | 1818790465269399552 |
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| author | C Halakoo J Nowroozi P Pakzad H Koohsari M Seyyed |
| author_facet | C Halakoo J Nowroozi P Pakzad H Koohsari M Seyyed |
| author_sort | C Halakoo |
| collection | DOAJ |
| description | Background and Objective: Water resident bacteria are potentially important reservoir of antibiotic resistance genes. This study was performed to evaluate the antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran.
Methods: In this descriptive study, 26 samples from Ziarat river water (13 samples pre & 13 samples post treatment) and 36 samples from Azadshahr area springs water (18 samples pre & 18 samples post treatment) were collected. 75 numbers of Escherichia coli bacterium samples (50 isolated from river and 25 isolated from springs) identified and isolated from raw waters of Golestan province, northern Iran by MPN method via differential tests. Susceptibility of Escherichia coli strains to 11 antibiotics (Amoxicillin / Clavulanic acid, Ampicillin, Imipenem, Cefalotin, Cefotaxime, Gentamicin, Amikacin, Tetracycline, Nalidixic acid, Ciprofloxacin and Trimethoprim / Sulfamethoxazole) was assayed by disk diffusion Kirby & Bauer’s method.
Results: 14 spring's raw water samples and 12 river raw water samples contained Escherichia coli. All of the river and springs samples assayed free from Escherichia coli post treatment. All of the Escherichia coli strains isolated from samples showed the similar phenotypical resistance against to surveyed 11 antibiotics. The most significance resistance to Ampicillin (river 94% & springs 88%), Amoxicillin / Clavulanic acid (river 76% & springs 80%), Tetracycline (river 14% & springs 16%) and Cefalotin (river 8% & springs 16%) viewed. Resistance to Trimethoprim / Sulfamethoxazole (8%), Nalidixic acid (2%) and Ciprofloxacin (2%) just viewed in river samples. All of the river and spring isolates were sensitive to Imipenem, Cefotaxime, Gentamicin and Amikacin and demonstrated intermediate resistance to others antibiotics.
Conclusion: Treatment of raw water from springs and rivers caused the eradication of Escherichia coli. As regard to observed phenotypical resistance in springs’ raw waters, presumably with lack of treatment springs’ raw water can be caused the transmission of antibiotic resistance to human body. |
| first_indexed | 2024-12-18T14:55:53Z |
| format | Article |
| id | doaj.art-460e5e15f5e54c608df2a27149cf2fe8 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-12-18T14:55:53Z |
| publishDate | 2016-12-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-460e5e15f5e54c608df2a27149cf2fe82022-12-21T21:04:04ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802016-12-011849499Antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern IranC Halakoo0J Nowroozi1P Pakzad2H Koohsari3M Seyyed4 Ph.D Candidate in Microbiology, Department of Microbiology, Faculty of Bioscience, Tehran North Branch, Islamic Azad University, Tehran, Iran Professor, Department of Microbiology, Faculty of Bioscience, Tehran North Branch, Islamic Azad University, Tehran, Iran Professor, Department of Microbiology, Faculty of Bioscience, Tehran North Branch, Islamic Azad University, Tehran, Iran Assistant Professor, Department of Microbiology, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran M.Sc in Environment Engineering-Water Resources, Faculty of Environment and Energy, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran Background and Objective: Water resident bacteria are potentially important reservoir of antibiotic resistance genes. This study was performed to evaluate the antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran. Methods: In this descriptive study, 26 samples from Ziarat river water (13 samples pre & 13 samples post treatment) and 36 samples from Azadshahr area springs water (18 samples pre & 18 samples post treatment) were collected. 75 numbers of Escherichia coli bacterium samples (50 isolated from river and 25 isolated from springs) identified and isolated from raw waters of Golestan province, northern Iran by MPN method via differential tests. Susceptibility of Escherichia coli strains to 11 antibiotics (Amoxicillin / Clavulanic acid, Ampicillin, Imipenem, Cefalotin, Cefotaxime, Gentamicin, Amikacin, Tetracycline, Nalidixic acid, Ciprofloxacin and Trimethoprim / Sulfamethoxazole) was assayed by disk diffusion Kirby & Bauer’s method. Results: 14 spring's raw water samples and 12 river raw water samples contained Escherichia coli. All of the river and springs samples assayed free from Escherichia coli post treatment. All of the Escherichia coli strains isolated from samples showed the similar phenotypical resistance against to surveyed 11 antibiotics. The most significance resistance to Ampicillin (river 94% & springs 88%), Amoxicillin / Clavulanic acid (river 76% & springs 80%), Tetracycline (river 14% & springs 16%) and Cefalotin (river 8% & springs 16%) viewed. Resistance to Trimethoprim / Sulfamethoxazole (8%), Nalidixic acid (2%) and Ciprofloxacin (2%) just viewed in river samples. All of the river and spring isolates were sensitive to Imipenem, Cefotaxime, Gentamicin and Amikacin and demonstrated intermediate resistance to others antibiotics. Conclusion: Treatment of raw water from springs and rivers caused the eradication of Escherichia coli. As regard to observed phenotypical resistance in springs’ raw waters, presumably with lack of treatment springs’ raw water can be caused the transmission of antibiotic resistance to human body.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-952&slc_lang=en&sid=1Antibiotic resistanceEscherichia coliRaw water |
| spellingShingle | C Halakoo J Nowroozi P Pakzad H Koohsari M Seyyed Antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran مجله دانشگاه علوم پزشکی گرگان Antibiotic resistance Escherichia coli Raw water |
| title | Antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran |
| title_full | Antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran |
| title_fullStr | Antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran |
| title_full_unstemmed | Antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran |
| title_short | Antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran |
| title_sort | antibiotic resistance in escherichia coli strains isolated from raw waters of golestan province northern iran |
| topic | Antibiotic resistance Escherichia coli Raw water |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-952&slc_lang=en&sid=1 |
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