First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso
Abstract Background Vulvovaginal candidiasis is an important cause of morbidity among women due to Candida species. In the last decades, resistance to azoles, first-line antifungals has increased. One molecular mechanism of azole resistance by Candida involves mutations in the ERG11 gene encoding la...
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| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-022-07619-5 |
| _version_ | 1811215700711178240 |
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| author | Essi Etonam Dovo Théodora Mahoukèdè Zohoncon Sessi Frida Tovo Serge Théophile Soubeiga Isabelle Touwendpoulimdé Kiendrebeogo Albert Théophane Yonli Rogomenona Alice Ouedraogo Amana Mètuor Dabire Florencia Wendkuuni Djigma Christelle Wendyam Nadembega Marius Belemgnegre Paul Ouedraogo Dorcas Obiri-Yeboah Jacques Simpore |
| author_facet | Essi Etonam Dovo Théodora Mahoukèdè Zohoncon Sessi Frida Tovo Serge Théophile Soubeiga Isabelle Touwendpoulimdé Kiendrebeogo Albert Théophane Yonli Rogomenona Alice Ouedraogo Amana Mètuor Dabire Florencia Wendkuuni Djigma Christelle Wendyam Nadembega Marius Belemgnegre Paul Ouedraogo Dorcas Obiri-Yeboah Jacques Simpore |
| author_sort | Essi Etonam Dovo |
| collection | DOAJ |
| description | Abstract Background Vulvovaginal candidiasis is an important cause of morbidity among women due to Candida species. In the last decades, resistance to azoles, first-line antifungals has increased. One molecular mechanism of azole resistance by Candida involves mutations in the ERG11 gene encoding lanosterol 14-α-demethylase, the target enzyme. This study was conducted to identify the clinical Candida species associated in vulvovaginal candidiasis; to determine the rate of antifungal resistance among Candida albicans isolates and to determine mutated ERG11 gene at Saint Camille Hospital in Ouagadougou, Burkina Faso. Methods Antifungals susceptibility were performed using Kirby–Bauer disk diffusion method. ERG11 gene was detected using conventional PCR in C. albicans isolates resistant to at least one azole. Results Out of 262 clinical strains isolated, C. albicans accounted for 59.90%, followed by Candida glabrata 27.86%, Candida famata 7.25%, Candida tropicalis 3.05% and Saccharomyces cerevisiae 1.91%. Resistance rate of fluconazole to C. albicans was 59.54%. ERG11 gene was found in 9.79% of 92 C. albicans strains resistant to azoles. Conclusions This detection of mutated ERG11 gene in C. albicans is the first in Burkina Faso and may be a cause of azole resistance in recurrent Candida vulvovaginitis. |
| first_indexed | 2024-04-12T06:26:50Z |
| format | Article |
| id | doaj.art-460f9098ed784559abd0f1a9f642806a |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-12T06:26:50Z |
| publishDate | 2022-08-01 |
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| series | BMC Infectious Diseases |
| spelling | doaj.art-460f9098ed784559abd0f1a9f642806a2022-12-22T03:44:07ZengBMCBMC Infectious Diseases1471-23342022-08-012211910.1186/s12879-022-07619-5First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina FasoEssi Etonam Dovo0Théodora Mahoukèdè Zohoncon1Sessi Frida Tovo2Serge Théophile Soubeiga3Isabelle Touwendpoulimdé Kiendrebeogo4Albert Théophane Yonli5Rogomenona Alice Ouedraogo6Amana Mètuor Dabire7Florencia Wendkuuni Djigma8Christelle Wendyam Nadembega9Marius Belemgnegre10Paul Ouedraogo11Dorcas Obiri-Yeboah12Jacques Simpore13Molecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBOMolecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBOMolecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBOResearch Institute for Health Sciences (IRSS)Molecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBOBiomolecular Research Center Pietro Annigoni (CERBA)Molecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBOUniversity of DedougouMolecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBOMolecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBOSaint Camille Hospital of Ouagadougou (HOSCO)Saint Camille Hospital of Ouagadougou (HOSCO)Department of Microbiology and Immunology, School of Medical Sciences, University of Cape CoastMolecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBOAbstract Background Vulvovaginal candidiasis is an important cause of morbidity among women due to Candida species. In the last decades, resistance to azoles, first-line antifungals has increased. One molecular mechanism of azole resistance by Candida involves mutations in the ERG11 gene encoding lanosterol 14-α-demethylase, the target enzyme. This study was conducted to identify the clinical Candida species associated in vulvovaginal candidiasis; to determine the rate of antifungal resistance among Candida albicans isolates and to determine mutated ERG11 gene at Saint Camille Hospital in Ouagadougou, Burkina Faso. Methods Antifungals susceptibility were performed using Kirby–Bauer disk diffusion method. ERG11 gene was detected using conventional PCR in C. albicans isolates resistant to at least one azole. Results Out of 262 clinical strains isolated, C. albicans accounted for 59.90%, followed by Candida glabrata 27.86%, Candida famata 7.25%, Candida tropicalis 3.05% and Saccharomyces cerevisiae 1.91%. Resistance rate of fluconazole to C. albicans was 59.54%. ERG11 gene was found in 9.79% of 92 C. albicans strains resistant to azoles. Conclusions This detection of mutated ERG11 gene in C. albicans is the first in Burkina Faso and may be a cause of azole resistance in recurrent Candida vulvovaginitis.https://doi.org/10.1186/s12879-022-07619-5ERG11 geneCandida albicansAzole resistanceVulvovaginitisBurkina Faso |
| spellingShingle | Essi Etonam Dovo Théodora Mahoukèdè Zohoncon Sessi Frida Tovo Serge Théophile Soubeiga Isabelle Touwendpoulimdé Kiendrebeogo Albert Théophane Yonli Rogomenona Alice Ouedraogo Amana Mètuor Dabire Florencia Wendkuuni Djigma Christelle Wendyam Nadembega Marius Belemgnegre Paul Ouedraogo Dorcas Obiri-Yeboah Jacques Simpore First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso BMC Infectious Diseases ERG11 gene Candida albicans Azole resistance Vulvovaginitis Burkina Faso |
| title | First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso |
| title_full | First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso |
| title_fullStr | First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso |
| title_full_unstemmed | First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso |
| title_short | First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso |
| title_sort | first detection of mutated erg11 gene in vulvovaginal candida albicans isolates at ouagadougou burkina faso |
| topic | ERG11 gene Candida albicans Azole resistance Vulvovaginitis Burkina Faso |
| url | https://doi.org/10.1186/s12879-022-07619-5 |
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