Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells
The rice cell suspension culture system is a good way to produce recombinant human proteins, owing to its high biosafety and low production cost. Human Octamer-binding Transcription Factor 4 (Oct4) is a fundamental transcription factor responsible for maintaining human pluripotent embryonic stem cel...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1409 |
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| author | Li-Fen Huang Desyanti Saulina Sinaga Chia-Chun Tan Shu-Ju Micky Hsieh Chi-Hung Huang |
| author_facet | Li-Fen Huang Desyanti Saulina Sinaga Chia-Chun Tan Shu-Ju Micky Hsieh Chi-Hung Huang |
| author_sort | Li-Fen Huang |
| collection | DOAJ |
| description | The rice cell suspension culture system is a good way to produce recombinant human proteins, owing to its high biosafety and low production cost. Human Octamer-binding Transcription Factor 4 (Oct4) is a fundamental transcription factor responsible for maintaining human pluripotent embryonic stem cells. Recombinant Oct4 protein has been used to induce pluripotent stem cells. In this study, recombinant Oct4 proteins are produced via a sugar starvation-inducible <i>αAmy3</i>/<i>RAmy3D</i> promoter–signal peptide-based rice recombinant protein expression system. <i>Oct4</i> mRNAs accumulate in the transgenic rice suspension cells under sugar starvation. The Oct4 recombinant protein is detected in the transgenic rice suspension cells, and its highest yield is approximately 0.41% of total cellular soluble proteins after one day of sugar starvation. The rice cell-synthesized recombinant human Oct4 protein show DNA-binding activity in vitro, which implies that the protein structure is correct for enabling specific binding to the target DNA motif. |
| first_indexed | 2024-03-09T06:20:35Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T06:20:35Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-460fcab8a76f421692675e4146a1a1ab2023-12-03T11:47:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223140910.3390/ijms22031409Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension CellsLi-Fen Huang0Desyanti Saulina Sinaga1Chia-Chun Tan2Shu-Ju Micky Hsieh3Chi-Hung Huang4Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Zhongli, Taoyuan County 320, TaiwanGraduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Zhongli, Taoyuan County 320, TaiwanGraduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Zhongli, Taoyuan County 320, TaiwanTaiwan Advance Bio-Pharmaceutical Inc., 12F, No. 25, Ln. 169, Kangning St, Xizhi Dist., New Taipei City 221, TaiwanTaiwan Advance Bio-Pharmaceutical Inc., 12F, No. 25, Ln. 169, Kangning St, Xizhi Dist., New Taipei City 221, TaiwanThe rice cell suspension culture system is a good way to produce recombinant human proteins, owing to its high biosafety and low production cost. Human Octamer-binding Transcription Factor 4 (Oct4) is a fundamental transcription factor responsible for maintaining human pluripotent embryonic stem cells. Recombinant Oct4 protein has been used to induce pluripotent stem cells. In this study, recombinant Oct4 proteins are produced via a sugar starvation-inducible <i>αAmy3</i>/<i>RAmy3D</i> promoter–signal peptide-based rice recombinant protein expression system. <i>Oct4</i> mRNAs accumulate in the transgenic rice suspension cells under sugar starvation. The Oct4 recombinant protein is detected in the transgenic rice suspension cells, and its highest yield is approximately 0.41% of total cellular soluble proteins after one day of sugar starvation. The rice cell-synthesized recombinant human Oct4 protein show DNA-binding activity in vitro, which implies that the protein structure is correct for enabling specific binding to the target DNA motif.https://www.mdpi.com/1422-0067/22/3/1409<i>αAmy3</i> promoterrecombinant human embryonic transcription factorhuman Oct4rice cell suspension culture system |
| spellingShingle | Li-Fen Huang Desyanti Saulina Sinaga Chia-Chun Tan Shu-Ju Micky Hsieh Chi-Hung Huang Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells International Journal of Molecular Sciences <i>αAmy3</i> promoter recombinant human embryonic transcription factor human Oct4 rice cell suspension culture system |
| title | Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells |
| title_full | Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells |
| title_fullStr | Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells |
| title_full_unstemmed | Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells |
| title_short | Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells |
| title_sort | expression of recombinant human octamer binding transcription factor 4 in rice suspension cells |
| topic | <i>αAmy3</i> promoter recombinant human embryonic transcription factor human Oct4 rice cell suspension culture system |
| url | https://www.mdpi.com/1422-0067/22/3/1409 |
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