Structural characterization of mucin O-glycosylation may provide important information to help prevent colorectal tumor recurrence
Although colorectal cancer (CRC) is a preventable and curable disease if early stage tumors are removed, it still represents the second cause of cancer-related death worldwide. Surgical resection is the only curative treatment but once operated the patient is either subjected to adjuvant chemotherap...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-10-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00217/full |
| _version_ | 1811239208470183936 |
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| author | Adriana eMihalache Adriana eMihalache Jean-François eDelplanque Bélinda eRingot Destrez Cindy eWavelet Pierre eGosset Bertrand eNunes Sophie eGroux Degroote Renaud eLéonard Catherine eRobbe Masselot |
| author_facet | Adriana eMihalache Adriana eMihalache Jean-François eDelplanque Bélinda eRingot Destrez Cindy eWavelet Pierre eGosset Bertrand eNunes Sophie eGroux Degroote Renaud eLéonard Catherine eRobbe Masselot |
| author_sort | Adriana eMihalache |
| collection | DOAJ |
| description | Although colorectal cancer (CRC) is a preventable and curable disease if early stage tumors are removed, it still represents the second cause of cancer-related death worldwide. Surgical resection is the only curative treatment but once operated the patient is either subjected to adjuvant chemotherapy or not, depending on the invasiveness of the cancer and risks of recurrence. In this context, we investigated, by mass spectrometry, alterations in the repertoire of glycosylation of mucins from colorectal tumors of various stages, grades and recurrence status. Tumors were also compared with their counterparts in resection margins from the same patients and with healthy controls.The obtained data showed an important decrease in the level of expression of sialylated core 3 based O-glycans in tumors correlated with an increase in sialylated core 1 structures. No correlation was established between stages of the tumor samples and mucin O-glycosylation. However, with the notable exception of sialyl Tn antigens, tumors with recurrence presented a milder alteration of glycosylation profile than tumors without recurrence. These results suggest that mucin O-glycans from tumors with recurrence might mimic a healthier physiological situation, hence deceiving the immune defense system. |
| first_indexed | 2024-04-12T12:55:43Z |
| format | Article |
| id | doaj.art-460fe5b5bbae4c05a9e5524595f964aa |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T12:55:43Z |
| publishDate | 2015-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-460fe5b5bbae4c05a9e5524595f964aa2022-12-22T03:32:19ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2015-10-01510.3389/fonc.2015.00217167521Structural characterization of mucin O-glycosylation may provide important information to help prevent colorectal tumor recurrenceAdriana eMihalache0Adriana eMihalache1Jean-François eDelplanque2Bélinda eRingot Destrez3Cindy eWavelet4Pierre eGosset5Bertrand eNunes6Sophie eGroux Degroote7Renaud eLéonard8Catherine eRobbe Masselot9Structural and Functional Glycobiology Unit, University of LilleHôpital saint Vincent de PaulStructural and Functional Glycobiology Unit, University of LilleStructural and Functional Glycobiology Unit, University of LilleStructural and Functional Glycobiology Unit, University of LilleHôpital saint Vincent de PaulHôpital Saint Philibert de LilleStructural and Functional Glycobiology Unit, University of LilleStructural and Functional Glycobiology Unit, University of LilleStructural and Functional Glycobiology Unit, University of LilleAlthough colorectal cancer (CRC) is a preventable and curable disease if early stage tumors are removed, it still represents the second cause of cancer-related death worldwide. Surgical resection is the only curative treatment but once operated the patient is either subjected to adjuvant chemotherapy or not, depending on the invasiveness of the cancer and risks of recurrence. In this context, we investigated, by mass spectrometry, alterations in the repertoire of glycosylation of mucins from colorectal tumors of various stages, grades and recurrence status. Tumors were also compared with their counterparts in resection margins from the same patients and with healthy controls.The obtained data showed an important decrease in the level of expression of sialylated core 3 based O-glycans in tumors correlated with an increase in sialylated core 1 structures. No correlation was established between stages of the tumor samples and mucin O-glycosylation. However, with the notable exception of sialyl Tn antigens, tumors with recurrence presented a milder alteration of glycosylation profile than tumors without recurrence. These results suggest that mucin O-glycans from tumors with recurrence might mimic a healthier physiological situation, hence deceiving the immune defense system.http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00217/fullGlycosylationRecurrencecolorectal cancerprognosishuman intestinal mucins |
| spellingShingle | Adriana eMihalache Adriana eMihalache Jean-François eDelplanque Bélinda eRingot Destrez Cindy eWavelet Pierre eGosset Bertrand eNunes Sophie eGroux Degroote Renaud eLéonard Catherine eRobbe Masselot Structural characterization of mucin O-glycosylation may provide important information to help prevent colorectal tumor recurrence Frontiers in Oncology Glycosylation Recurrence colorectal cancer prognosis human intestinal mucins |
| title | Structural characterization of mucin O-glycosylation may provide important information to help prevent colorectal tumor recurrence |
| title_full | Structural characterization of mucin O-glycosylation may provide important information to help prevent colorectal tumor recurrence |
| title_fullStr | Structural characterization of mucin O-glycosylation may provide important information to help prevent colorectal tumor recurrence |
| title_full_unstemmed | Structural characterization of mucin O-glycosylation may provide important information to help prevent colorectal tumor recurrence |
| title_short | Structural characterization of mucin O-glycosylation may provide important information to help prevent colorectal tumor recurrence |
| title_sort | structural characterization of mucin o glycosylation may provide important information to help prevent colorectal tumor recurrence |
| topic | Glycosylation Recurrence colorectal cancer prognosis human intestinal mucins |
| url | http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00217/full |
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