Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD
In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927123001776 |
| _version_ | 1797394810226606080 |
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| author | Yaqun Liu Liyun Lin Huagui Wei Qiulan Luo Peikui Yang Mouquan Liu Zhonghe Wang Xianghui Zou Hui Zhu Guangcai Zha Junjun Sun Yuzhong Zheng Min Lin |
| author_facet | Yaqun Liu Liyun Lin Huagui Wei Qiulan Luo Peikui Yang Mouquan Liu Zhonghe Wang Xianghui Zou Hui Zhu Guangcai Zha Junjun Sun Yuzhong Zheng Min Lin |
| author_sort | Yaqun Liu |
| collection | DOAJ |
| description | In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable for on-site testing. Therefore, there is an urgent need for a rapid, sensitive, high specificity and high sensitivity on-site meat detection method. This study is the first to apply RPA combined with CRISPR/Cas12a technology to the field of multiple meat identification. The system developed by parameter optimization can achieve specific detection of chicken, duck, beef, pork and lamb with a minimum target sequence copy number as low as 1 × 100 copies/μL for 60 min at a constant temperature. LFD test results can be directly observed with the naked eye, with the characteristics of fast, portable and simple operation, which is extremely in line with current needs. In conclusion, the meat identification RPA-CRISPR/Cas12a-LFD system established in this study has shown promising applications in the field of meat detection, with a profound impact on meat quality, and provides a model for other food safety control programs. |
| first_indexed | 2024-03-09T00:25:07Z |
| format | Article |
| id | doaj.art-46121a55a89844dca3f936e4ddb0063b |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-03-09T00:25:07Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-46121a55a89844dca3f936e4ddb0063b2023-12-12T04:36:07ZengElsevierCurrent Research in Food Science2665-92712023-01-017100609Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFDYaqun Liu0Liyun Lin1Huagui Wei2Qiulan Luo3Peikui Yang4Mouquan Liu5Zhonghe Wang6Xianghui Zou7Hui Zhu8Guangcai Zha9Junjun Sun10Yuzhong Zheng11Min Lin12School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaShool of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, Guangxi, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR ChinaSchool of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR China; Shool of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, Guangxi, PR China; Corresponding author. School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China.School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China; Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou, Guangdong, PR China; Shool of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, Guangxi, PR China; Corresponding author. School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, PR China.In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable for on-site testing. Therefore, there is an urgent need for a rapid, sensitive, high specificity and high sensitivity on-site meat detection method. This study is the first to apply RPA combined with CRISPR/Cas12a technology to the field of multiple meat identification. The system developed by parameter optimization can achieve specific detection of chicken, duck, beef, pork and lamb with a minimum target sequence copy number as low as 1 × 100 copies/μL for 60 min at a constant temperature. LFD test results can be directly observed with the naked eye, with the characteristics of fast, portable and simple operation, which is extremely in line with current needs. In conclusion, the meat identification RPA-CRISPR/Cas12a-LFD system established in this study has shown promising applications in the field of meat detection, with a profound impact on meat quality, and provides a model for other food safety control programs.http://www.sciencedirect.com/science/article/pii/S2665927123001776Meat identificationRPACRISPR/Cas12aLFD |
| spellingShingle | Yaqun Liu Liyun Lin Huagui Wei Qiulan Luo Peikui Yang Mouquan Liu Zhonghe Wang Xianghui Zou Hui Zhu Guangcai Zha Junjun Sun Yuzhong Zheng Min Lin Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD Current Research in Food Science Meat identification RPA CRISPR/Cas12a LFD |
| title | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
| title_full | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
| title_fullStr | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
| title_full_unstemmed | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
| title_short | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
| title_sort | design and development of a rapid meat detection system based on rpa crispr cas12a lfd |
| topic | Meat identification RPA CRISPR/Cas12a LFD |
| url | http://www.sciencedirect.com/science/article/pii/S2665927123001776 |
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