Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides
Abstract Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate poly(hydroxyalkanoates) (PHA). In this study, the potential of the bacterium for co‐production of exopoly...
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| Format: | Article |
| Language: | English |
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Wiley
2021-02-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.1160 |
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| author | Luis Romero Soto Habib Thabet Reuben Maghembe Denise Gameiro Doan Van‐Thuoc Tarek Dishisha Rajni Hatti‐Kaul |
| author_facet | Luis Romero Soto Habib Thabet Reuben Maghembe Denise Gameiro Doan Van‐Thuoc Tarek Dishisha Rajni Hatti‐Kaul |
| author_sort | Luis Romero Soto |
| collection | DOAJ |
| description | Abstract Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate poly(hydroxyalkanoates) (PHA). In this study, the potential of the bacterium for co‐production of exopolysaccharides (EPS) and PHA was investigated. Genome sequence analysis of the closely related Yangia sp. CCB‐M3 isolated from mangroves in Malaysia revealed genes encoding enzymes participating in different EPS biosynthetic pathways. The effects of various cultivation parameters on the production of EPS and PHA were studied. The highest level of EPS (288 mg/L) was achieved using sucrose and yeast extract with 5% NaCl and 120 mM phosphate salts but with modest PHA accumulation (32% of cell dry weight, 1.3 g/L). Growth on fructose yielded the highest PHA concentration (85% of CDW, 3.3 g/L) at 90 mM phosphate and higher molecular weight EPS at 251 mg/L yield at 120 mM phosphate concentration. Analysis of EPS showed a predominance of glucose, followed by fructose and galactose, and minor amounts of acidic sugars. |
| first_indexed | 2024-12-17T03:47:27Z |
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| id | doaj.art-46138ec03409462a93bd41ffbc2a6745 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-17T03:47:27Z |
| publishDate | 2021-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-46138ec03409462a93bd41ffbc2a67452022-12-21T22:04:51ZengWileyMicrobiologyOpen2045-88272021-02-01101n/an/a10.1002/mbo3.1160Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharidesLuis Romero Soto0Habib Thabet1Reuben Maghembe2Denise Gameiro3Doan Van‐Thuoc4Tarek Dishisha5Rajni Hatti‐Kaul6Division of Biotechnology Center for Chemistry and Chemical Engineering Lund University Lund SwedenDivision of Biotechnology Center for Chemistry and Chemical Engineering Lund University Lund SwedenDivision of Biotechnology Center for Chemistry and Chemical Engineering Lund University Lund SwedenDivision of Biotechnology Center for Chemistry and Chemical Engineering Lund University Lund SwedenDepartment of Biotechnology and Microbiology Faculty of Biology Hanoi National University of Education Hanoi VietnamDepartment of Pharmaceutical Microbiology and Immunology Faculty of Pharmacy Beni‐Suef University Beni‐Suef EgyptDivision of Biotechnology Center for Chemistry and Chemical Engineering Lund University Lund SwedenAbstract Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate poly(hydroxyalkanoates) (PHA). In this study, the potential of the bacterium for co‐production of exopolysaccharides (EPS) and PHA was investigated. Genome sequence analysis of the closely related Yangia sp. CCB‐M3 isolated from mangroves in Malaysia revealed genes encoding enzymes participating in different EPS biosynthetic pathways. The effects of various cultivation parameters on the production of EPS and PHA were studied. The highest level of EPS (288 mg/L) was achieved using sucrose and yeast extract with 5% NaCl and 120 mM phosphate salts but with modest PHA accumulation (32% of cell dry weight, 1.3 g/L). Growth on fructose yielded the highest PHA concentration (85% of CDW, 3.3 g/L) at 90 mM phosphate and higher molecular weight EPS at 251 mg/L yield at 120 mM phosphate concentration. Analysis of EPS showed a predominance of glucose, followed by fructose and galactose, and minor amounts of acidic sugars.https://doi.org/10.1002/mbo3.1160co‐productionexopolysaccharidemoderate halophilepoly(3‐hydroxybutyrate)Yangia sp. ND199 |
| spellingShingle | Luis Romero Soto Habib Thabet Reuben Maghembe Denise Gameiro Doan Van‐Thuoc Tarek Dishisha Rajni Hatti‐Kaul Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides MicrobiologyOpen co‐production exopolysaccharide moderate halophile poly(3‐hydroxybutyrate) Yangia sp. ND199 |
| title | Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides |
| title_full | Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides |
| title_fullStr | Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides |
| title_full_unstemmed | Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides |
| title_short | Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides |
| title_sort | metabolic potential of the moderate halophile yangia sp nd199 for co production of polyhydroxyalkanoates and exopolysaccharides |
| topic | co‐production exopolysaccharide moderate halophile poly(3‐hydroxybutyrate) Yangia sp. ND199 |
| url | https://doi.org/10.1002/mbo3.1160 |
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