Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat
The aim of this study was to evaluate the effects of buffalo milk and cow milk on lipid metabolism in obese mice. Milk composition analysis showed fat, protein, and total solid content in buffalo milk was higher than cow milk, while the lactose content of buffalo milk was lower than cow milk. After...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.841800/full |
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| author | Maocheng Jiang Zitong Meng Zhiqiang Cheng Kang Zhan Xiaoyu Ma Tianyu Yang Yinghao Huang Qi Yan Xiaoxiao Gong Guoqi Zhao Guoqi Zhao Guoqi Zhao |
| author_facet | Maocheng Jiang Zitong Meng Zhiqiang Cheng Kang Zhan Xiaoyu Ma Tianyu Yang Yinghao Huang Qi Yan Xiaoxiao Gong Guoqi Zhao Guoqi Zhao Guoqi Zhao |
| author_sort | Maocheng Jiang |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effects of buffalo milk and cow milk on lipid metabolism in obese mice. Milk composition analysis showed fat, protein, and total solid content in buffalo milk was higher than cow milk, while the lactose content of buffalo milk was lower than cow milk. After milk metabolite extraction and LC-MS/MS analysis, differential metabolites were mainly enriched in “linoleic acid metabolism pathways,” “pentose and glucuronate interconversion pathways,” and “metabolism of xenobiotics by cytochrome P450 pathways.” We fed three groups of C57BL/6J mice (n = 6 per group) for 5 weeks: (1) high-fat diet group (HFD group); (2) high-fat diet + buffalo milk group (HBM group); and (3) high-fat diet + cow milk group (HCM group). Our results showed that body weight of mice was significantly decreased in HBM and HCM groups from 1 to 4 weeks compared with the HFD group. The mRNA expression of ACAA2, ACACB, and SLC27A5 genes involved in the lipid metabolism in liver tissue were significantly elevated in HCM group, relatively to HFD and HBM group. In addition, the adipocyte number, size and lipid accumulation in the liver were significantly decreased in HCM group compared with the HFD group by H&E staining and oil red O staining, but was not change in HBM group. The mRNA levels of TNF-α and IL-1β inflammatory genes were significantly increased in HBM group, relatively to HFD and HCM group, which is consistent with results from inflammatory cell infiltration and tissue disruption by colon tissue sections. In conclusion, dietary supplementation of cow milk has beneficial effects on loss of weight and lipid metabolism in obese mice. |
| first_indexed | 2024-12-10T09:45:57Z |
| format | Article |
| id | doaj.art-4613d23a86f54f729c3cf683d177a6df |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-10T09:45:57Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-4613d23a86f54f729c3cf683d177a6df2022-12-22T01:53:50ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-04-01910.3389/fnut.2022.841800841800Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High FatMaocheng Jiang0Zitong Meng1Zhiqiang Cheng2Kang Zhan3Xiaoyu Ma4Tianyu Yang5Yinghao Huang6Qi Yan7Xiaoxiao Gong8Guoqi Zhao9Guoqi Zhao10Guoqi Zhao11College of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Institute of Animal Culture Collection and Application, Yangzhou University, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Ministry of Education of China, Yangzhou, ChinaThe aim of this study was to evaluate the effects of buffalo milk and cow milk on lipid metabolism in obese mice. Milk composition analysis showed fat, protein, and total solid content in buffalo milk was higher than cow milk, while the lactose content of buffalo milk was lower than cow milk. After milk metabolite extraction and LC-MS/MS analysis, differential metabolites were mainly enriched in “linoleic acid metabolism pathways,” “pentose and glucuronate interconversion pathways,” and “metabolism of xenobiotics by cytochrome P450 pathways.” We fed three groups of C57BL/6J mice (n = 6 per group) for 5 weeks: (1) high-fat diet group (HFD group); (2) high-fat diet + buffalo milk group (HBM group); and (3) high-fat diet + cow milk group (HCM group). Our results showed that body weight of mice was significantly decreased in HBM and HCM groups from 1 to 4 weeks compared with the HFD group. The mRNA expression of ACAA2, ACACB, and SLC27A5 genes involved in the lipid metabolism in liver tissue were significantly elevated in HCM group, relatively to HFD and HBM group. In addition, the adipocyte number, size and lipid accumulation in the liver were significantly decreased in HCM group compared with the HFD group by H&E staining and oil red O staining, but was not change in HBM group. The mRNA levels of TNF-α and IL-1β inflammatory genes were significantly increased in HBM group, relatively to HFD and HCM group, which is consistent with results from inflammatory cell infiltration and tissue disruption by colon tissue sections. In conclusion, dietary supplementation of cow milk has beneficial effects on loss of weight and lipid metabolism in obese mice.https://www.frontiersin.org/articles/10.3389/fnut.2022.841800/fullbuffalomilkmetabonomiclipid metabolisminflammatory |
| spellingShingle | Maocheng Jiang Zitong Meng Zhiqiang Cheng Kang Zhan Xiaoyu Ma Tianyu Yang Yinghao Huang Qi Yan Xiaoxiao Gong Guoqi Zhao Guoqi Zhao Guoqi Zhao Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat Frontiers in Nutrition buffalo milk metabonomic lipid metabolism inflammatory |
| title | Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat |
| title_full | Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat |
| title_fullStr | Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat |
| title_full_unstemmed | Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat |
| title_short | Effects of Buffalo Milk and Cow Milk on Lipid Metabolism in Obese Mice Induced by High Fat |
| title_sort | effects of buffalo milk and cow milk on lipid metabolism in obese mice induced by high fat |
| topic | buffalo milk metabonomic lipid metabolism inflammatory |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.841800/full |
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