Multiplexed single-molecule force spectroscopy using a centrifuge
Single molecule force spectroscopy (SMFS) has limitations in throughput and the ability to repeatedly interrogate single bonds. Here the authors repurpose a benchtop centrifuge and use DNA nanoswitches to enable high throughput SMFS capable of repeatedly measuring forces of single molecular pairs.
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2016-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11026 |
| _version_ | 1818978097694769152 |
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| author | Darren Yang Andrew Ward Ken Halvorsen Wesley P. Wong |
| author_facet | Darren Yang Andrew Ward Ken Halvorsen Wesley P. Wong |
| author_sort | Darren Yang |
| collection | DOAJ |
| description | Single molecule force spectroscopy (SMFS) has limitations in throughput and the ability to repeatedly interrogate single bonds. Here the authors repurpose a benchtop centrifuge and use DNA nanoswitches to enable high throughput SMFS capable of repeatedly measuring forces of single molecular pairs. |
| first_indexed | 2024-12-20T16:38:13Z |
| format | Article |
| id | doaj.art-461600ab61c74653a81a639f445b8670 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-20T16:38:13Z |
| publishDate | 2016-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-461600ab61c74653a81a639f445b86702022-12-21T19:33:05ZengNature PortfolioNature Communications2041-17232016-03-01711710.1038/ncomms11026Multiplexed single-molecule force spectroscopy using a centrifugeDarren Yang0Andrew Ward1Ken Halvorsen2Wesley P. Wong3School of engineering and applied sciences, Harvard UniversityProgram in Cellular and Molecular Medicine, Boston Children’s HospitalProgram in Cellular and Molecular Medicine, Boston Children’s HospitalProgram in Cellular and Molecular Medicine, Boston Children’s HospitalSingle molecule force spectroscopy (SMFS) has limitations in throughput and the ability to repeatedly interrogate single bonds. Here the authors repurpose a benchtop centrifuge and use DNA nanoswitches to enable high throughput SMFS capable of repeatedly measuring forces of single molecular pairs.https://doi.org/10.1038/ncomms11026 |
| spellingShingle | Darren Yang Andrew Ward Ken Halvorsen Wesley P. Wong Multiplexed single-molecule force spectroscopy using a centrifuge Nature Communications |
| title | Multiplexed single-molecule force spectroscopy using a centrifuge |
| title_full | Multiplexed single-molecule force spectroscopy using a centrifuge |
| title_fullStr | Multiplexed single-molecule force spectroscopy using a centrifuge |
| title_full_unstemmed | Multiplexed single-molecule force spectroscopy using a centrifuge |
| title_short | Multiplexed single-molecule force spectroscopy using a centrifuge |
| title_sort | multiplexed single molecule force spectroscopy using a centrifuge |
| url | https://doi.org/10.1038/ncomms11026 |
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