Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells
Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is anisothiocyanate found in some cruciferous vegetables, especiallybroccoli. Sulforaphane has been shown to displayanti-cancer properties against various cancer cell lines. Matrixmetalloproteinase-9 (MMP-9), which degrades the extracellularm...
| Asıl Yazarlar: | , , , , , , , , , , |
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| Materyal Türü: | Makale |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Korean Society for Biochemistry and Molecular Biology
2013-04-01
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| Seri Bilgileri: | BMB Reports |
| Konular: | |
| Online Erişim: | http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTBAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni00JTVEMTMwNDI2MjAzMF8lMjgyMDEtMjA2JTI5Qk1CXzEyLTE2MC5wZGY= |
| Özet: | Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is anisothiocyanate found in some cruciferous vegetables, especiallybroccoli. Sulforaphane has been shown to displayanti-cancer properties against various cancer cell lines. Matrixmetalloproteinase-9 (MMP-9), which degrades the extracellularmatrix (ECM), plays an important role in cancer cell invasion.In this study, we investigated the effect of sulforaphane on12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9expression and cell invasion in MCF-7 cells. TPA-inducedMMP-9 expression and cell invasion were decreased bysulforaphane treatment. TPA substantially increased NF-κB andAP-1 DNA binding activity. Pre-treatment with sulforaphaneinhibited TPA-stimulated NF-κB binding activity, but not AP-1binding activity. In addition, we found that sulforaphanesuppressed NF-κB activation, by inhibiting phosphorylation ofIκB in TPA-treated MCF-7 cells. In this study, we demonstratedthat the inhibition of TPA-induced MMP-9 expression and cellinvasion by sulforaphane was mediated by the suppression ofthe NF-κB pathway in MCF-7 cells. [BMB Reports 2013; 46(4):201-206] |
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| ISSN: | 1976-6696 1976-670X |