Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata
ABSTRACTEchinocandin resistance in Candida glabrata poses a serious clinical challenge. The underlying resistance mechanism of a pan-echinocandin-resistant C. glabrata isolate (strain L74) was investigated in this study. FKS mutants carrying specific mutations found in L74 were reconstructed by the...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1684209 |
| _version_ | 1797385755714125824 |
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| author | Xin Hou Kelley R. Healey Erika Shor Milena Kordalewska Cristina Jiménez Ortigosa Padmaja Paderu Meng Xiao He Wang Ying Zhao Li-Yan Lin Yan-Hai Zhang Yong-Zhe Li Ying-Chun Xu David S. Perlin Yanan Zhao |
| author_facet | Xin Hou Kelley R. Healey Erika Shor Milena Kordalewska Cristina Jiménez Ortigosa Padmaja Paderu Meng Xiao He Wang Ying Zhao Li-Yan Lin Yan-Hai Zhang Yong-Zhe Li Ying-Chun Xu David S. Perlin Yanan Zhao |
| author_sort | Xin Hou |
| collection | DOAJ |
| description | ABSTRACTEchinocandin resistance in Candida glabrata poses a serious clinical challenge. The underlying resistance mechanism of a pan-echinocandin-resistant C. glabrata isolate (strain L74) was investigated in this study. FKS mutants carrying specific mutations found in L74 were reconstructed by the Alt-R CRISPR-Cas9 system (Fks1 WT/Fks2-E655K, strain CRISPR 31) and site-directed mutagenesis (strain fks1Δ/Fks2-E655K). Sequence analysis of strain L74 revealed a premature stop codon W508stop in FKS1 and an E655K mutation preceding the hotspot 1 region in FKS2. Introduction of the Fks2-E655K mutation in ATCC 2001 (strain CRISPR 31) conferred a modest reduction in susceptibility. However, the same FKS2 mutation in the fks1Δ background (strain fks1Δ/Fks2-E655K) resulted in high levels of resistance to echinocandins. Glucan synthase isolated from L74 was dramatically less sensitive to micafungin (MCF) relative to ATCC 2001. Both FKS1/FKS2 transcript ratios and Fks1/Fks2 protein ratios were significantly lower in L74 and fks1Δ/Fks2-E655K compared to ATCC 2001 and CRISPR 31 (P <0.05). Mice challenged with CRISPR 31 and fks1Δ/Fks2-E655K mutants failed to respond to MCF. In conclusion, the high-level of echinocandin resistance in the clinical isolate of C. glabrata L74 was concluded to result from the combination of null function of Fks1 and the point mutation E655K in Fks2. |
| first_indexed | 2024-03-08T21:58:51Z |
| format | Article |
| id | doaj.art-4617bf9f1b1d4a9d867399b3ce74b219 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-08T21:58:51Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-4617bf9f1b1d4a9d867399b3ce74b2192023-12-19T16:09:57ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-01811619162510.1080/22221751.2019.1684209Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrataXin Hou0Kelley R. Healey1Erika Shor2Milena Kordalewska3Cristina Jiménez Ortigosa4Padmaja Paderu5Meng Xiao6He Wang7Ying Zhao8Li-Yan Lin9Yan-Hai Zhang10Yong-Zhe Li11Ying-Chun Xu12David S. Perlin13Yanan Zhao14Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaDepartment of Biology, William Paterson University, Wayne, NJ, USACenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USACenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USACenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USACenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USADepartment of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaSchool of Medicine, Peking University Health Science Center, Beijing, People’s Republic of ChinaCentral Laboratory, Hebei Yanda Hospital, Langfang, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaDepartment of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaCenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USACenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USAABSTRACTEchinocandin resistance in Candida glabrata poses a serious clinical challenge. The underlying resistance mechanism of a pan-echinocandin-resistant C. glabrata isolate (strain L74) was investigated in this study. FKS mutants carrying specific mutations found in L74 were reconstructed by the Alt-R CRISPR-Cas9 system (Fks1 WT/Fks2-E655K, strain CRISPR 31) and site-directed mutagenesis (strain fks1Δ/Fks2-E655K). Sequence analysis of strain L74 revealed a premature stop codon W508stop in FKS1 and an E655K mutation preceding the hotspot 1 region in FKS2. Introduction of the Fks2-E655K mutation in ATCC 2001 (strain CRISPR 31) conferred a modest reduction in susceptibility. However, the same FKS2 mutation in the fks1Δ background (strain fks1Δ/Fks2-E655K) resulted in high levels of resistance to echinocandins. Glucan synthase isolated from L74 was dramatically less sensitive to micafungin (MCF) relative to ATCC 2001. Both FKS1/FKS2 transcript ratios and Fks1/Fks2 protein ratios were significantly lower in L74 and fks1Δ/Fks2-E655K compared to ATCC 2001 and CRISPR 31 (P <0.05). Mice challenged with CRISPR 31 and fks1Δ/Fks2-E655K mutants failed to respond to MCF. In conclusion, the high-level of echinocandin resistance in the clinical isolate of C. glabrata L74 was concluded to result from the combination of null function of Fks1 and the point mutation E655K in Fks2.https://www.tandfonline.com/doi/10.1080/22221751.2019.1684209Candida glabrataFKSechinocandin resistanceCRISPR-Cas9resistance mechanism |
| spellingShingle | Xin Hou Kelley R. Healey Erika Shor Milena Kordalewska Cristina Jiménez Ortigosa Padmaja Paderu Meng Xiao He Wang Ying Zhao Li-Yan Lin Yan-Hai Zhang Yong-Zhe Li Ying-Chun Xu David S. Perlin Yanan Zhao Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata Emerging Microbes and Infections Candida glabrata FKS echinocandin resistance CRISPR-Cas9 resistance mechanism |
| title | Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata |
| title_full | Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata |
| title_fullStr | Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata |
| title_full_unstemmed | Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata |
| title_short | Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata |
| title_sort | novel fks1 and fks2 modifications in a high level echinocandin resistant clinical isolate of candida glabrata |
| topic | Candida glabrata FKS echinocandin resistance CRISPR-Cas9 resistance mechanism |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1684209 |
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