A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast.
Chd proteins are ATP-dependent chromatin remodeling enzymes implicated in biological functions from transcriptional elongation to control of pluripotency. Previous studies of the Chd1 subclass of these proteins have implicated them in diverse roles in gene expression including functions during initi...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3395613?pdf=render |
| _version_ | 1818693066578460672 |
|---|---|
| author | Marta Radman-Livaja Tiffani K Quan Lourdes Valenzuela Jennifer A Armstrong Tibor van Welsem Taesoo Kim Laura J Lee Stephen Buratowski Fred van Leeuwen Oliver J Rando Grant A Hartzog |
| author_facet | Marta Radman-Livaja Tiffani K Quan Lourdes Valenzuela Jennifer A Armstrong Tibor van Welsem Taesoo Kim Laura J Lee Stephen Buratowski Fred van Leeuwen Oliver J Rando Grant A Hartzog |
| author_sort | Marta Radman-Livaja |
| collection | DOAJ |
| description | Chd proteins are ATP-dependent chromatin remodeling enzymes implicated in biological functions from transcriptional elongation to control of pluripotency. Previous studies of the Chd1 subclass of these proteins have implicated them in diverse roles in gene expression including functions during initiation, elongation, and termination. Furthermore, some evidence has suggested a role for Chd1 in replication-independent histone exchange or assembly. Here, we examine roles of Chd1 in replication-independent dynamics of histone H3 in both Drosophila and yeast. We find evidence of a role for Chd1 in H3 dynamics in both organisms. Using genome-wide ChIP-on-chip analysis, we find that Chd1 influences histone turnover at the 5' and 3' ends of genes, accelerating H3 replacement at the 5' ends of genes while protecting the 3' ends of genes from excessive H3 turnover. Although consistent with a direct role for Chd1 in exchange, these results may indicate that Chd1 stabilizes nucleosomes perturbed by transcription. Curiously, we observe a strong effect of gene length on Chd1's effects on H3 turnover. Finally, we show that Chd1 also affects histone modification patterns over genes, likely as a consequence of its effects on histone replacement. Taken together, our results emphasize a role for Chd1 in histone replacement in both budding yeast and Drosophila melanogaster, and surprisingly they show that the major effects of Chd1 on turnover occur at the 3' ends of genes. |
| first_indexed | 2024-12-17T13:07:47Z |
| format | Article |
| id | doaj.art-4618d4ff39504e22860d8deb0e6a7b99 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-17T13:07:47Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-4618d4ff39504e22860d8deb0e6a7b992022-12-21T21:47:12ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042012-01-0187e100281110.1371/journal.pgen.1002811A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast.Marta Radman-LivajaTiffani K QuanLourdes ValenzuelaJennifer A ArmstrongTibor van WelsemTaesoo KimLaura J LeeStephen BuratowskiFred van LeeuwenOliver J RandoGrant A HartzogChd proteins are ATP-dependent chromatin remodeling enzymes implicated in biological functions from transcriptional elongation to control of pluripotency. Previous studies of the Chd1 subclass of these proteins have implicated them in diverse roles in gene expression including functions during initiation, elongation, and termination. Furthermore, some evidence has suggested a role for Chd1 in replication-independent histone exchange or assembly. Here, we examine roles of Chd1 in replication-independent dynamics of histone H3 in both Drosophila and yeast. We find evidence of a role for Chd1 in H3 dynamics in both organisms. Using genome-wide ChIP-on-chip analysis, we find that Chd1 influences histone turnover at the 5' and 3' ends of genes, accelerating H3 replacement at the 5' ends of genes while protecting the 3' ends of genes from excessive H3 turnover. Although consistent with a direct role for Chd1 in exchange, these results may indicate that Chd1 stabilizes nucleosomes perturbed by transcription. Curiously, we observe a strong effect of gene length on Chd1's effects on H3 turnover. Finally, we show that Chd1 also affects histone modification patterns over genes, likely as a consequence of its effects on histone replacement. Taken together, our results emphasize a role for Chd1 in histone replacement in both budding yeast and Drosophila melanogaster, and surprisingly they show that the major effects of Chd1 on turnover occur at the 3' ends of genes.http://europepmc.org/articles/PMC3395613?pdf=render |
| spellingShingle | Marta Radman-Livaja Tiffani K Quan Lourdes Valenzuela Jennifer A Armstrong Tibor van Welsem Taesoo Kim Laura J Lee Stephen Buratowski Fred van Leeuwen Oliver J Rando Grant A Hartzog A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast. PLoS Genetics |
| title | A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast. |
| title_full | A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast. |
| title_fullStr | A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast. |
| title_full_unstemmed | A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast. |
| title_short | A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast. |
| title_sort | key role for chd1 in histone h3 dynamics at the 3 ends of long genes in yeast |
| url | http://europepmc.org/articles/PMC3395613?pdf=render |
| work_keys_str_mv | AT martaradmanlivaja akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT tiffanikquan akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT lourdesvalenzuela akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT jenniferaarmstrong akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT tiborvanwelsem akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT taesookim akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT laurajlee akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT stephenburatowski akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT fredvanleeuwen akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT oliverjrando akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT grantahartzog akeyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT martaradmanlivaja keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT tiffanikquan keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT lourdesvalenzuela keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT jenniferaarmstrong keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT tiborvanwelsem keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT taesookim keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT laurajlee keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT stephenburatowski keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT fredvanleeuwen keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT oliverjrando keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast AT grantahartzog keyroleforchd1inhistoneh3dynamicsatthe3endsoflonggenesinyeast |