Assisted Extraction with Cyclodextrins as a Way of Improving the Antidiabetic Activity of <i>Actinidia</i> Leaves
Five varieties of <i>Actinidia</i> leaves (Geneva, Jumbo, Ken’s Red, Kijivska Hibridna, and Sentyabraskaya) were analyzed. The profiles of active compounds were determined, namely quercetin, rutin, epicatechin, chlorogenic acid, and kaempferol, in the raw material. Suspecting that the ra...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/11/2473 |
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| author | Szymon Sip Anna Gościniak Piotr Szulc Jarosław Walkowiak Judyta Cielecka-Piontek |
| author_facet | Szymon Sip Anna Gościniak Piotr Szulc Jarosław Walkowiak Judyta Cielecka-Piontek |
| author_sort | Szymon Sip |
| collection | DOAJ |
| description | Five varieties of <i>Actinidia</i> leaves (Geneva, Jumbo, Ken’s Red, Kijivska Hibridna, and Sentyabraskaya) were analyzed. The profiles of active compounds were determined, namely quercetin, rutin, epicatechin, chlorogenic acid, and kaempferol, in the raw material. Suspecting that the raw material might prove important in the treatment of diabetes, the authors assessed the antioxidant activity and the ability to inhibit enzymes responsible for the development of diabetes (α-glucosidase and α-amylase). As a result of the conducted analysis, the Ken’s Red variety was indicated as having the highest biological activity (DPPH IC<sub>50</sub> = 0.332 ± 0.048; FRAP IC<sub>0.5</sub> = 0.064 ± 0.005; α-glucosidase inhibition IC<sub>50</sub> = 0.098 ± 0.007; α-amylase inhibition IC<sub>50</sub> = 0.083 ± 0.004). In order to increase the efficiency of the extraction of active compounds from Ken’s Red variety leaves, cyclodextrins (α-CD, β-CD, and γ-CD) were used as extraction process enhancers. The obtained results showed a significant increase in the contents of extracted active compounds. In addition, the type of CD used enhanced the extraction of selected compounds (quercetin, kaempferol, rutin, chlorogenic acid, and epicatechin. This study shows that the application of cyclodextrin-based extraction significantly improved the leaf activity of the Ken’s Red variety (DPPH IC<sub>50</sub> = 0.160 ± 0.019; FRAP IC<sub>0.5</sub> = 0.008 ± 0.001; α-glucosidase inhibition IC<sub>50</sub> = 0.040 ± 0.002; α-amylase inhibition IC<sub>50</sub> = 0.012 ± 0.003). |
| first_indexed | 2024-03-09T18:04:37Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T18:04:37Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-461ae2879fb44899aa735443abf0358a2023-11-24T09:36:24ZengMDPI AGPharmaceutics1999-49232022-11-011411247310.3390/pharmaceutics14112473Assisted Extraction with Cyclodextrins as a Way of Improving the Antidiabetic Activity of <i>Actinidia</i> LeavesSzymon Sip0Anna Gościniak1Piotr Szulc2Jarosław Walkowiak3Judyta Cielecka-Piontek4Department of Pharmacognosy, Faculty of Pharmacy, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznań, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznań, PolandDepartment of Agronomy, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, PolandDepartment of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Szpitalna 27/33, 60-572 Poznań, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznań, PolandFive varieties of <i>Actinidia</i> leaves (Geneva, Jumbo, Ken’s Red, Kijivska Hibridna, and Sentyabraskaya) were analyzed. The profiles of active compounds were determined, namely quercetin, rutin, epicatechin, chlorogenic acid, and kaempferol, in the raw material. Suspecting that the raw material might prove important in the treatment of diabetes, the authors assessed the antioxidant activity and the ability to inhibit enzymes responsible for the development of diabetes (α-glucosidase and α-amylase). As a result of the conducted analysis, the Ken’s Red variety was indicated as having the highest biological activity (DPPH IC<sub>50</sub> = 0.332 ± 0.048; FRAP IC<sub>0.5</sub> = 0.064 ± 0.005; α-glucosidase inhibition IC<sub>50</sub> = 0.098 ± 0.007; α-amylase inhibition IC<sub>50</sub> = 0.083 ± 0.004). In order to increase the efficiency of the extraction of active compounds from Ken’s Red variety leaves, cyclodextrins (α-CD, β-CD, and γ-CD) were used as extraction process enhancers. The obtained results showed a significant increase in the contents of extracted active compounds. In addition, the type of CD used enhanced the extraction of selected compounds (quercetin, kaempferol, rutin, chlorogenic acid, and epicatechin. This study shows that the application of cyclodextrin-based extraction significantly improved the leaf activity of the Ken’s Red variety (DPPH IC<sub>50</sub> = 0.160 ± 0.019; FRAP IC<sub>0.5</sub> = 0.008 ± 0.001; α-glucosidase inhibition IC<sub>50</sub> = 0.040 ± 0.002; α-amylase inhibition IC<sub>50</sub> = 0.012 ± 0.003).https://www.mdpi.com/1999-4923/14/11/2473<i>Actinidia</i>enzyme inhibitioncyclodextrinextractiondiabeteskiwi |
| spellingShingle | Szymon Sip Anna Gościniak Piotr Szulc Jarosław Walkowiak Judyta Cielecka-Piontek Assisted Extraction with Cyclodextrins as a Way of Improving the Antidiabetic Activity of <i>Actinidia</i> Leaves Pharmaceutics <i>Actinidia</i> enzyme inhibition cyclodextrin extraction diabetes kiwi |
| title | Assisted Extraction with Cyclodextrins as a Way of Improving the Antidiabetic Activity of <i>Actinidia</i> Leaves |
| title_full | Assisted Extraction with Cyclodextrins as a Way of Improving the Antidiabetic Activity of <i>Actinidia</i> Leaves |
| title_fullStr | Assisted Extraction with Cyclodextrins as a Way of Improving the Antidiabetic Activity of <i>Actinidia</i> Leaves |
| title_full_unstemmed | Assisted Extraction with Cyclodextrins as a Way of Improving the Antidiabetic Activity of <i>Actinidia</i> Leaves |
| title_short | Assisted Extraction with Cyclodextrins as a Way of Improving the Antidiabetic Activity of <i>Actinidia</i> Leaves |
| title_sort | assisted extraction with cyclodextrins as a way of improving the antidiabetic activity of i actinidia i leaves |
| topic | <i>Actinidia</i> enzyme inhibition cyclodextrin extraction diabetes kiwi |
| url | https://www.mdpi.com/1999-4923/14/11/2473 |
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