Genome-Wide Association Study Identifies Resistance Loci for Bacterial Blight in a Collection of Asian Temperate <i>Japonica</i> Rice Germplasm
Growing resistant rice cultivars is the most effective strategy to control bacterial blight (BB), a devastating disease caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>). Screening resistant germplasm and identifying resistance (<i>R</i&g...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/10/8810 |
| Summary: | Growing resistant rice cultivars is the most effective strategy to control bacterial blight (BB), a devastating disease caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>). Screening resistant germplasm and identifying resistance (<i>R</i>) genes are prerequisites for breeding resistant rice cultivars. We conducted a genome-wide association study (GWAS) to detect quantitative trait loci (QTL) associated with BB resistance using 359 East Asian temperate <i>Japonica</i> accessions inoculated with two Chinese <i>Xoo</i> strains (KS6-6 and GV) and one Philippine <i>Xoo</i> strain (PXO99<sup>A</sup>). Based on the 55K SNPs Array dataset of the 359 <i>Japonica</i> accessions, eight QTL were identified on rice chromosomes 1, 2, 4, 10, and 11. Four of the QTL coincided with previously reported QTL, and four were novel loci. Six <i>R</i> genes were localized in the <i>qBBV-11.1</i>, <i>qBBV-11.2</i>, and <i>qBBV-11.3</i> loci on chromosome 11 in this <i>Japonica</i> collection. Haplotype analysis revealed candidate genes associated with BB resistance in each QTL. Notably, <i>LOC_Os11g47290</i> in <i>qBBV-11.3</i>, encoding a leucine-rich repeat receptor-like kinase, was a candidate gene associated with resistance to the virulent strain GV. Knockout mutants of Nipponbare with the susceptible haplotype of <i>LOC_Os11g47290</i> exhibited significantly improved BB resistance. These results will be useful for cloning BB resistance genes and breeding resistant rice cultivars. |
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| ISSN: | 1661-6596 1422-0067 |