Whole-Genome and Expression Analyses of Bamboo Aquaporin Genes Reveal Their Functions Involved in Maintaining Diurnal Water Balance in Bamboo Shoots
Water supply is essential for maintaining normal physiological function during the rapid growth of bamboo. Aquaporins (AQPs) play crucial roles in water transport for plant growth and development. Although 26 <i>PeAQP</i>s in bamboo have been reported, the aquaporin-led mechanism of main...
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MDPI AG
2018-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/7/11/195 |
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| author | Huayu Sun Sining Wang Yongfeng Lou Chenglei Zhu Hansheng Zhao Ying Li Xueping Li Zhimin Gao |
| author_facet | Huayu Sun Sining Wang Yongfeng Lou Chenglei Zhu Hansheng Zhao Ying Li Xueping Li Zhimin Gao |
| author_sort | Huayu Sun |
| collection | DOAJ |
| description | Water supply is essential for maintaining normal physiological function during the rapid growth of bamboo. Aquaporins (AQPs) play crucial roles in water transport for plant growth and development. Although 26 <i>PeAQP</i>s in bamboo have been reported, the aquaporin-led mechanism of maintaining diurnal water balance in bamboo shoots remains unclear. In this study, a total of 63 <i>PeAQP</i>s were identified, based on the updated genome of moso bamboo (<i>Phyllostachys edulis</i>), including 22 <i>PePIP</i>s, 20 <i>PeTIP</i>s, 17 <i>PeNIP</i>s, and 4 <i>PeSIP</i>s. All of the <i>PeAQP</i>s were differently expressed in 26 different tissues of moso bamboo, based on RNA sequencing (RNA-seq) data. The root pressure in shoots showed circadian rhythm changes, with positive values at night and negative values in the daytime. The quantitative real-time PCR (qRT-PCR) result showed that 25 <i>PeAQP</i>s were detected in the base part of the shoots, and most of them demonstrated diurnal rhythm changes. The expression levels of some <i>PeAQP</i>s were significantly correlated with the root pressure. Of the 86 sugar transport genes, 33 had positive co-expression relationships with 27 <i>PeAQP</i>s. Two root pressure-correlated <i>PeAQP</i>s, <i>PeTIP4;1</i> and <i>PeTIP4;2</i>, were confirmed to be highly expressed in the parenchyma and epidermal cells of bamboo culm, and in the epidermis, pith, and primary xylem of bamboo roots by in situ hybridization. The authors’ findings provide new insights and a possible aquaporin-led mechanism for bamboo fast growth. |
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| language | English |
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| publishDate | 2018-11-01 |
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| series | Cells |
| spelling | doaj.art-46237acb5d59409a9a4c7808086da6352023-09-03T10:30:08ZengMDPI AGCells2073-44092018-11-0171119510.3390/cells7110195cells7110195Whole-Genome and Expression Analyses of Bamboo Aquaporin Genes Reveal Their Functions Involved in Maintaining Diurnal Water Balance in Bamboo ShootsHuayu Sun0Sining Wang1Yongfeng Lou2Chenglei Zhu3Hansheng Zhao4Ying Li5Xueping Li6Zhimin Gao7State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing 100102, ChinaState Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing 100102, ChinaJiangxi Academy of Forestry, Nanchang 330013, ChinaState Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing 100102, ChinaState Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing 100102, ChinaState Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing 100102, ChinaState Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing 100102, ChinaState Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing 100102, ChinaWater supply is essential for maintaining normal physiological function during the rapid growth of bamboo. Aquaporins (AQPs) play crucial roles in water transport for plant growth and development. Although 26 <i>PeAQP</i>s in bamboo have been reported, the aquaporin-led mechanism of maintaining diurnal water balance in bamboo shoots remains unclear. In this study, a total of 63 <i>PeAQP</i>s were identified, based on the updated genome of moso bamboo (<i>Phyllostachys edulis</i>), including 22 <i>PePIP</i>s, 20 <i>PeTIP</i>s, 17 <i>PeNIP</i>s, and 4 <i>PeSIP</i>s. All of the <i>PeAQP</i>s were differently expressed in 26 different tissues of moso bamboo, based on RNA sequencing (RNA-seq) data. The root pressure in shoots showed circadian rhythm changes, with positive values at night and negative values in the daytime. The quantitative real-time PCR (qRT-PCR) result showed that 25 <i>PeAQP</i>s were detected in the base part of the shoots, and most of them demonstrated diurnal rhythm changes. The expression levels of some <i>PeAQP</i>s were significantly correlated with the root pressure. Of the 86 sugar transport genes, 33 had positive co-expression relationships with 27 <i>PeAQP</i>s. Two root pressure-correlated <i>PeAQP</i>s, <i>PeTIP4;1</i> and <i>PeTIP4;2</i>, were confirmed to be highly expressed in the parenchyma and epidermal cells of bamboo culm, and in the epidermis, pith, and primary xylem of bamboo roots by in situ hybridization. The authors’ findings provide new insights and a possible aquaporin-led mechanism for bamboo fast growth.https://www.mdpi.com/2073-4409/7/11/195aquaporinsAQPsbambooroot pressurefast growth |
| spellingShingle | Huayu Sun Sining Wang Yongfeng Lou Chenglei Zhu Hansheng Zhao Ying Li Xueping Li Zhimin Gao Whole-Genome and Expression Analyses of Bamboo Aquaporin Genes Reveal Their Functions Involved in Maintaining Diurnal Water Balance in Bamboo Shoots Cells aquaporins AQPs bamboo root pressure fast growth |
| title | Whole-Genome and Expression Analyses of Bamboo Aquaporin Genes Reveal Their Functions Involved in Maintaining Diurnal Water Balance in Bamboo Shoots |
| title_full | Whole-Genome and Expression Analyses of Bamboo Aquaporin Genes Reveal Their Functions Involved in Maintaining Diurnal Water Balance in Bamboo Shoots |
| title_fullStr | Whole-Genome and Expression Analyses of Bamboo Aquaporin Genes Reveal Their Functions Involved in Maintaining Diurnal Water Balance in Bamboo Shoots |
| title_full_unstemmed | Whole-Genome and Expression Analyses of Bamboo Aquaporin Genes Reveal Their Functions Involved in Maintaining Diurnal Water Balance in Bamboo Shoots |
| title_short | Whole-Genome and Expression Analyses of Bamboo Aquaporin Genes Reveal Their Functions Involved in Maintaining Diurnal Water Balance in Bamboo Shoots |
| title_sort | whole genome and expression analyses of bamboo aquaporin genes reveal their functions involved in maintaining diurnal water balance in bamboo shoots |
| topic | aquaporins AQPs bamboo root pressure fast growth |
| url | https://www.mdpi.com/2073-4409/7/11/195 |
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