Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin Wounds
The treatment of wounds using the body’s own resources is a promising approach to support the physiological regenerative process. To advance this concept, we evaluated the effect of nanofat (NF) on wound healing. For this purpose, full-thickness skin defects were created in dorsal skinfold chambers...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/2/851 |
| _version_ | 1797339804820570112 |
|---|---|
| author | Ettore Limido Andrea Weinzierl Emmanuel Ampofo Yves Harder Michael D. Menger Matthias W. Laschke |
| author_facet | Ettore Limido Andrea Weinzierl Emmanuel Ampofo Yves Harder Michael D. Menger Matthias W. Laschke |
| author_sort | Ettore Limido |
| collection | DOAJ |
| description | The treatment of wounds using the body’s own resources is a promising approach to support the physiological regenerative process. To advance this concept, we evaluated the effect of nanofat (NF) on wound healing. For this purpose, full-thickness skin defects were created in dorsal skinfold chambers of wild-type mice. These defects were filled with NF generated from the inguinal subcutaneous adipose tissue of green fluorescent protein (GFP)<sup>+</sup> donor mice, which was stabilized using platelet-rich plasma (PRP). Empty wounds and wounds solely filled with PRP served as controls. Wound closure, vascularization and formation of granulation tissue were repeatedly analyzed using stereomicroscopy, intravital fluorescence microscopy, histology and immunohistochemistry over an observation period of 14 days. PRP + NF-treated wounds exhibited accelerated vascularization and wound closure when compared to controls. This was primarily due to the fact that the grafted NF contained a substantial fraction of viable GFP<sup>+</sup> vascular and lymph vessel fragments, which interconnected with the GFP<sup>−</sup> vessels of the host tissue. Moreover, the switch from inflammatory M1- to regenerative M2-polarized macrophages was promoted in PRP + NF-treated wounds. These findings indicate that NF markedly accelerates and improves the wound healing process and, thus, represents a promising autologous product for future wound management. |
| first_indexed | 2024-03-08T09:53:42Z |
| format | Article |
| id | doaj.art-4624db71a1ed4898b411a12d0dbea207 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:53:42Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4624db71a1ed4898b411a12d0dbea2072024-01-29T13:55:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-0125285110.3390/ijms25020851Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin WoundsEttore Limido0Andrea Weinzierl1Emmanuel Ampofo2Yves Harder3Michael D. Menger4Matthias W. Laschke5Institute for Clinical and Experimental Surgery, Saarland University, 66421 Homburg, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, 66421 Homburg, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, 66421 Homburg, GermanyDepartment of Plastic, Reconstructive and Aesthetic Surgery, Ospedale Regionale di Lugano, Ente Ospedaliero Cantonale (EOC), 6900 Lugano, SwitzerlandInstitute for Clinical and Experimental Surgery, Saarland University, 66421 Homburg, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, 66421 Homburg, GermanyThe treatment of wounds using the body’s own resources is a promising approach to support the physiological regenerative process. To advance this concept, we evaluated the effect of nanofat (NF) on wound healing. For this purpose, full-thickness skin defects were created in dorsal skinfold chambers of wild-type mice. These defects were filled with NF generated from the inguinal subcutaneous adipose tissue of green fluorescent protein (GFP)<sup>+</sup> donor mice, which was stabilized using platelet-rich plasma (PRP). Empty wounds and wounds solely filled with PRP served as controls. Wound closure, vascularization and formation of granulation tissue were repeatedly analyzed using stereomicroscopy, intravital fluorescence microscopy, histology and immunohistochemistry over an observation period of 14 days. PRP + NF-treated wounds exhibited accelerated vascularization and wound closure when compared to controls. This was primarily due to the fact that the grafted NF contained a substantial fraction of viable GFP<sup>+</sup> vascular and lymph vessel fragments, which interconnected with the GFP<sup>−</sup> vessels of the host tissue. Moreover, the switch from inflammatory M1- to regenerative M2-polarized macrophages was promoted in PRP + NF-treated wounds. These findings indicate that NF markedly accelerates and improves the wound healing process and, thus, represents a promising autologous product for future wound management.https://www.mdpi.com/1422-0067/25/2/851wound healingnanofatplatelet-rich plasmavascularizationangiogenesislymph vessels |
| spellingShingle | Ettore Limido Andrea Weinzierl Emmanuel Ampofo Yves Harder Michael D. Menger Matthias W. Laschke Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin Wounds International Journal of Molecular Sciences wound healing nanofat platelet-rich plasma vascularization angiogenesis lymph vessels |
| title | Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin Wounds |
| title_full | Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin Wounds |
| title_fullStr | Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin Wounds |
| title_full_unstemmed | Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin Wounds |
| title_short | Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin Wounds |
| title_sort | nanofat accelerates and improves the vascularization lymphatic drainage and healing of full thickness murine skin wounds |
| topic | wound healing nanofat platelet-rich plasma vascularization angiogenesis lymph vessels |
| url | https://www.mdpi.com/1422-0067/25/2/851 |
| work_keys_str_mv | AT ettorelimido nanofatacceleratesandimprovesthevascularizationlymphaticdrainageandhealingoffullthicknessmurineskinwounds AT andreaweinzierl nanofatacceleratesandimprovesthevascularizationlymphaticdrainageandhealingoffullthicknessmurineskinwounds AT emmanuelampofo nanofatacceleratesandimprovesthevascularizationlymphaticdrainageandhealingoffullthicknessmurineskinwounds AT yvesharder nanofatacceleratesandimprovesthevascularizationlymphaticdrainageandhealingoffullthicknessmurineskinwounds AT michaeldmenger nanofatacceleratesandimprovesthevascularizationlymphaticdrainageandhealingoffullthicknessmurineskinwounds AT matthiaswlaschke nanofatacceleratesandimprovesthevascularizationlymphaticdrainageandhealingoffullthicknessmurineskinwounds |