Bovine Herpesvirus-1 Glycoprotein M Mediates the Translocation to the Golgi Apparatus and Packaging of VP8
VP8, the most abundant tegument protein of bovine herpesvirus-1 (BoHV-1), plays an important role in viral replication. According to our previous studies, VP8 localizes to the Golgi apparatus of BoHV-1-infected cells where it can be packaged into the virus; however, Golgi localization of VP8 does no...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/9/1985 |
| _version_ | 1797481341975003136 |
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| author | Soumya Sucharita Suresh Tikoo Sylvia van Drunen Littel-van den Hurk |
| author_facet | Soumya Sucharita Suresh Tikoo Sylvia van Drunen Littel-van den Hurk |
| author_sort | Soumya Sucharita |
| collection | DOAJ |
| description | VP8, the most abundant tegument protein of bovine herpesvirus-1 (BoHV-1), plays an important role in viral replication. According to our previous studies, VP8 localizes to the Golgi apparatus of BoHV-1-infected cells where it can be packaged into the virus; however, Golgi localization of VP8 does not occur outside of the context of infection. The goal of this study was to identify the viral factor(s) involved in the tropism of VP8 towards the Golgi. VP8 was found to interact with glycoprotein M (gM), and the VP8 and gM domains that are essential for this interaction were identified. VP8 and gM colocalized to the Golgi apparatus in BoHV-1-infected cells. In cells co-transfected with VP8- and gM-encoding plasmids, VP8 was also found to be localized to the Golgi, suggesting gM to be sufficient. The localization of VP8 to the Golgi was lost in cells infected with a gM deletion mutant, and the amount of VP8 incorporated into mature virus was significantly reduced. However, with the restoration of gM in a revertant virus, the localization to the Golgi and the amount of VP8 incorporated in the virions were restored. These results indicate that gM plays a critical role in VP8 subcellular localization to the Golgi and packaging into mature virions. |
| first_indexed | 2024-03-09T22:13:10Z |
| format | Article |
| id | doaj.art-4624e6da13fc441d9ed25ef07674dae8 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T22:13:10Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4624e6da13fc441d9ed25ef07674dae82023-11-23T19:27:37ZengMDPI AGViruses1999-49152022-09-01149198510.3390/v14091985Bovine Herpesvirus-1 Glycoprotein M Mediates the Translocation to the Golgi Apparatus and Packaging of VP8Soumya Sucharita0Suresh Tikoo1Sylvia van Drunen Littel-van den Hurk2Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK S7N 5E5, CanadaVaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, CanadaBiochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK S7N 5E5, CanadaVP8, the most abundant tegument protein of bovine herpesvirus-1 (BoHV-1), plays an important role in viral replication. According to our previous studies, VP8 localizes to the Golgi apparatus of BoHV-1-infected cells where it can be packaged into the virus; however, Golgi localization of VP8 does not occur outside of the context of infection. The goal of this study was to identify the viral factor(s) involved in the tropism of VP8 towards the Golgi. VP8 was found to interact with glycoprotein M (gM), and the VP8 and gM domains that are essential for this interaction were identified. VP8 and gM colocalized to the Golgi apparatus in BoHV-1-infected cells. In cells co-transfected with VP8- and gM-encoding plasmids, VP8 was also found to be localized to the Golgi, suggesting gM to be sufficient. The localization of VP8 to the Golgi was lost in cells infected with a gM deletion mutant, and the amount of VP8 incorporated into mature virus was significantly reduced. However, with the restoration of gM in a revertant virus, the localization to the Golgi and the amount of VP8 incorporated in the virions were restored. These results indicate that gM plays a critical role in VP8 subcellular localization to the Golgi and packaging into mature virions.https://www.mdpi.com/1999-4915/14/9/1985bovine herpesvirus-1tegumentVP8glycoprotein MGolgi localization |
| spellingShingle | Soumya Sucharita Suresh Tikoo Sylvia van Drunen Littel-van den Hurk Bovine Herpesvirus-1 Glycoprotein M Mediates the Translocation to the Golgi Apparatus and Packaging of VP8 Viruses bovine herpesvirus-1 tegument VP8 glycoprotein M Golgi localization |
| title | Bovine Herpesvirus-1 Glycoprotein M Mediates the Translocation to the Golgi Apparatus and Packaging of VP8 |
| title_full | Bovine Herpesvirus-1 Glycoprotein M Mediates the Translocation to the Golgi Apparatus and Packaging of VP8 |
| title_fullStr | Bovine Herpesvirus-1 Glycoprotein M Mediates the Translocation to the Golgi Apparatus and Packaging of VP8 |
| title_full_unstemmed | Bovine Herpesvirus-1 Glycoprotein M Mediates the Translocation to the Golgi Apparatus and Packaging of VP8 |
| title_short | Bovine Herpesvirus-1 Glycoprotein M Mediates the Translocation to the Golgi Apparatus and Packaging of VP8 |
| title_sort | bovine herpesvirus 1 glycoprotein m mediates the translocation to the golgi apparatus and packaging of vp8 |
| topic | bovine herpesvirus-1 tegument VP8 glycoprotein M Golgi localization |
| url | https://www.mdpi.com/1999-4915/14/9/1985 |
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