Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+
The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal and horticultural interests. The objective was to identify the culture media that support the best organogenesis and callogenic expressi...
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| Format: | Article |
| Language: | English |
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AcademicPres
2023-06-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13147 |
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| author | Hana BOKRI Samiha KAHLAOUI Kheiria HCINI Manel DHOUEIBI Fethia HARZALLAH-SKHIRI Sondes STAMBOULI-ESSASSI |
| author_facet | Hana BOKRI Samiha KAHLAOUI Kheiria HCINI Manel DHOUEIBI Fethia HARZALLAH-SKHIRI Sondes STAMBOULI-ESSASSI |
| author_sort | Hana BOKRI |
| collection | DOAJ |
| description |
The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal and horticultural interests. The objective was to identify the culture media that support the best organogenesis and callogenic expressions of four types of aerial explants; nodal and internodal segments and leaf portions, by varying the mineral composition of twenty culture media by adding increasing concentrations of Na+ (from 0-100 mM) and K+ (from 0-50 mM). After two months of culture, parameters relating to the various expressions of organogenesis and callogenesis were measured. Results showed that the K+ and Na+ interactions affect the explants development. The combination of high concentrations of Na+ (50, 100 mM) and K+ (50 mM) ions allowed the most important regeneration of the axillary shoots (70.6-100%), root neoformation (82.6-96.1%) and callus induction (76.3-100%). Rooted plantlets with well-developed axillary shoots have been successfully acclimatized with a 93% of survival rate. The selected media would allow a large-scale multiplication of this medicinal species, without adding exogenous phytohormones, and could be used for the micropropagation of other threatened halophyte species or for the production of callus; rich in secondary metabolites.
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| first_indexed | 2024-03-13T02:17:54Z |
| format | Article |
| id | doaj.art-46260923ff5945439956fa507b076721 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-03-13T02:17:54Z |
| publishDate | 2023-06-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-46260923ff5945439956fa507b0767212023-06-30T11:05:00ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092023-06-0151210.15835/nbha51213147Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+Hana BOKRI0Samiha KAHLAOUI1Kheiria HCINI2Manel DHOUEIBI3Fethia HARZALLAH-SKHIRI4Sondes STAMBOULI-ESSASSI5University of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 TunisUniversity of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 TunisUniversity of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 TunisUniversity of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 TunisUniversity of Monastir, High Institute of Biotechnology of Monastir, Laboratory of Bioresources: Integrative Biology and Valorization (LR14-ES06), 5000 MonastirUniversity of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 Tunis The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal and horticultural interests. The objective was to identify the culture media that support the best organogenesis and callogenic expressions of four types of aerial explants; nodal and internodal segments and leaf portions, by varying the mineral composition of twenty culture media by adding increasing concentrations of Na+ (from 0-100 mM) and K+ (from 0-50 mM). After two months of culture, parameters relating to the various expressions of organogenesis and callogenesis were measured. Results showed that the K+ and Na+ interactions affect the explants development. The combination of high concentrations of Na+ (50, 100 mM) and K+ (50 mM) ions allowed the most important regeneration of the axillary shoots (70.6-100%), root neoformation (82.6-96.1%) and callus induction (76.3-100%). Rooted plantlets with well-developed axillary shoots have been successfully acclimatized with a 93% of survival rate. The selected media would allow a large-scale multiplication of this medicinal species, without adding exogenous phytohormones, and could be used for the micropropagation of other threatened halophyte species or for the production of callus; rich in secondary metabolites. https://www.notulaebotanicae.ro/index.php/nbha/article/view/13147callogenesisLimbarda crithmoïdes Lorganogenesispotassiumsodium |
| spellingShingle | Hana BOKRI Samiha KAHLAOUI Kheiria HCINI Manel DHOUEIBI Fethia HARZALLAH-SKHIRI Sondes STAMBOULI-ESSASSI Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+ Notulae Botanicae Horti Agrobotanici Cluj-Napoca callogenesis Limbarda crithmoïdes L organogenesis potassium sodium |
| title | Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+ |
| title_full | Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+ |
| title_fullStr | Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+ |
| title_full_unstemmed | Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+ |
| title_short | Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+ |
| title_sort | induction of organogenesis and callogenesis in limbarda crithmoides l asteraceae explants cultured on ms media supplemented with various concentrations of na and k |
| topic | callogenesis Limbarda crithmoïdes L organogenesis potassium sodium |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13147 |
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