Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows
Objective Abnormally increased somatic cell counts (SCCs) in milk is usually a sign of bovine subclinical mastitis. Mutual interaction between the host and its associated microbiota plays an important role in developing such diseases. The main objective of this study was to explore the difference be...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2024-04-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-21-0495.pdf |
| _version_ | 1797208777475227648 |
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| author | Jinning Zhang Xueqin Liu Tahir Usman Yongjie Tang Siyuan Mi Wenlong Li Mengyou Yang Ying Yu |
| author_facet | Jinning Zhang Xueqin Liu Tahir Usman Yongjie Tang Siyuan Mi Wenlong Li Mengyou Yang Ying Yu |
| author_sort | Jinning Zhang |
| collection | DOAJ |
| description | Objective Abnormally increased somatic cell counts (SCCs) in milk is usually a sign of bovine subclinical mastitis. Mutual interaction between the host and its associated microbiota plays an important role in developing such diseases. The main objective of this study was to explore the difference between cows with elevated SCCs and healthy cattle from the perspective of host-microbe interplay. Methods A total of 31 milk samples and 23 bovine peripheral blood samples were collected from Holstein dairy cattle to conduct an integrated analysis of transcriptomic and metagenomics. Results The results showed that Ralstonia and Sphingomonas were enriched in cows with subclinical mastitis. The relative abundance of the two bacteria was positively correlated with the expression level of bovine transcobalamin 1 and uridine phosphorylase 1 encoding gene. Moreover, functional analysis revealed a distinct alternation in some important microbial biological processes. Conclusion These results reveal the relative abundance of Ralstonia and Sphingomonas other than common mastitis-causing pathogens varied from healthy cows to those with subclinical mastitis and might be associated with elevated SCCs. Potential association was observed between bovine milk microbiota composition and the transcriptional pattern of some genes, thus providing new insights to understand homeostasis of bovine udder. |
| first_indexed | 2024-03-07T14:29:56Z |
| format | Article |
| id | doaj.art-462b0e224746477aa514ba7275bf73b3 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-24T09:44:12Z |
| publishDate | 2024-04-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-462b0e224746477aa514ba7275bf73b32024-04-15T04:05:41ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352024-04-0137470971710.5713/ab.21.049524843Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cowsJinning Zhang0Xueqin Liu1Tahir Usman2Yongjie Tang3Siyuan Mi4Wenlong Li5Mengyou Yang6Ying Yu7 Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, 23200, Pakistan Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 ChinaObjective Abnormally increased somatic cell counts (SCCs) in milk is usually a sign of bovine subclinical mastitis. Mutual interaction between the host and its associated microbiota plays an important role in developing such diseases. The main objective of this study was to explore the difference between cows with elevated SCCs and healthy cattle from the perspective of host-microbe interplay. Methods A total of 31 milk samples and 23 bovine peripheral blood samples were collected from Holstein dairy cattle to conduct an integrated analysis of transcriptomic and metagenomics. Results The results showed that Ralstonia and Sphingomonas were enriched in cows with subclinical mastitis. The relative abundance of the two bacteria was positively correlated with the expression level of bovine transcobalamin 1 and uridine phosphorylase 1 encoding gene. Moreover, functional analysis revealed a distinct alternation in some important microbial biological processes. Conclusion These results reveal the relative abundance of Ralstonia and Sphingomonas other than common mastitis-causing pathogens varied from healthy cows to those with subclinical mastitis and might be associated with elevated SCCs. Potential association was observed between bovine milk microbiota composition and the transcriptional pattern of some genes, thus providing new insights to understand homeostasis of bovine udder.http://www.animbiosci.org/upload/pdf/ab-21-0495.pdfhost-microbe interplaymetagenomesubclinical mastitistranscriptome |
| spellingShingle | Jinning Zhang Xueqin Liu Tahir Usman Yongjie Tang Siyuan Mi Wenlong Li Mengyou Yang Ying Yu Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows Animal Bioscience host-microbe interplay metagenome subclinical mastitis transcriptome |
| title | Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows |
| title_full | Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows |
| title_fullStr | Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows |
| title_full_unstemmed | Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows |
| title_short | Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows |
| title_sort | integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows |
| topic | host-microbe interplay metagenome subclinical mastitis transcriptome |
| url | http://www.animbiosci.org/upload/pdf/ab-21-0495.pdf |
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