Follicle‐stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation
Previous studies have found follicle‐stimulating hormone (FSH) receptors on chondrocytes (cartilage cells), but the mechanism of FSH action on chondrocytes is not clear. The purpose of this experiment is to study whether FSH affects chondrocytes and how it causes changes in these cells. Our results...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-08-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13238 |
| _version_ | 1797787899719057408 |
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| author | Zhikun Huan Yan Wang Mengqi Zhang Xiujuan Zhang Yaping Liu Lei Kong Jin Xu |
| author_facet | Zhikun Huan Yan Wang Mengqi Zhang Xiujuan Zhang Yaping Liu Lei Kong Jin Xu |
| author_sort | Zhikun Huan |
| collection | DOAJ |
| description | Previous studies have found follicle‐stimulating hormone (FSH) receptors on chondrocytes (cartilage cells), but the mechanism of FSH action on chondrocytes is not clear. The purpose of this experiment is to study whether FSH affects chondrocytes and how it causes changes in these cells. Our results show that osteoarthritis became worse after FSH injection in the knee joint of mice. After the stimulation of chondrocytes by FSH, a total of 664 up‐regulated genes, such as Col12a1 and Col1a1, and 644 down‐regulated genes, such as MGP, were screened by transcriptomics. A subset of extracellular matrix (ECM)‐related genes and pathways underwent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and the downregulation of MGP, the upregulation of EGR1 and Col1a1, and the increase of IL‐6 were verified. It was also observed that FSH can inhibit the cAMP/PKA and MKK4/JNK signaling pathway. In conclusion, we demonstrated that FSH can increase cartilage inflammatory response and promote chondrocyte dedifferentiation by inhibiting the cAMP/PKA and MKK4/JNK signaling pathways. |
| first_indexed | 2024-03-13T01:27:59Z |
| format | Article |
| id | doaj.art-462cc6fa033b43c2829678eba392b2e4 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-13T01:27:59Z |
| publishDate | 2021-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-462cc6fa033b43c2829678eba392b2e42023-07-04T10:16:03ZengWileyFEBS Open Bio2211-54632021-08-011182292230310.1002/2211-5463.13238Follicle‐stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiationZhikun Huan0Yan Wang1Mengqi Zhang2Xiujuan Zhang3Yaping Liu4Lei Kong5Jin Xu6Department of Endocrinology Shandong Provincial Hospital Cheeloo College of Medicine Shandong University Jinan ChinaDepartment of Endocrinology Shandong Provincial Hospital Cheeloo College of Medicine Shandong University Jinan ChinaDepartment of Endocrinology Shandong Provincial Hospital Cheeloo College of Medicine Shandong University Jinan ChinaDepartment of Endocrinology Shandong Provincial Hospital Cheeloo College of Medicine Shandong University Jinan ChinaDepartment of Endocrinology Jining No.1 People’s Hospital Jining ChinaDepartment of Endocrinology Shandong Provincial Hospital Cheeloo College of Medicine Shandong University Jinan ChinaDepartment of Endocrinology Shandong Provincial Hospital Cheeloo College of Medicine Shandong University Jinan ChinaPrevious studies have found follicle‐stimulating hormone (FSH) receptors on chondrocytes (cartilage cells), but the mechanism of FSH action on chondrocytes is not clear. The purpose of this experiment is to study whether FSH affects chondrocytes and how it causes changes in these cells. Our results show that osteoarthritis became worse after FSH injection in the knee joint of mice. After the stimulation of chondrocytes by FSH, a total of 664 up‐regulated genes, such as Col12a1 and Col1a1, and 644 down‐regulated genes, such as MGP, were screened by transcriptomics. A subset of extracellular matrix (ECM)‐related genes and pathways underwent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and the downregulation of MGP, the upregulation of EGR1 and Col1a1, and the increase of IL‐6 were verified. It was also observed that FSH can inhibit the cAMP/PKA and MKK4/JNK signaling pathway. In conclusion, we demonstrated that FSH can increase cartilage inflammatory response and promote chondrocyte dedifferentiation by inhibiting the cAMP/PKA and MKK4/JNK signaling pathways.https://doi.org/10.1002/2211-5463.13238chondrocytesdedifferentiationfollicle‐stimulating hormoneIL‐6osteoarthritistype I collagen |
| spellingShingle | Zhikun Huan Yan Wang Mengqi Zhang Xiujuan Zhang Yaping Liu Lei Kong Jin Xu Follicle‐stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation FEBS Open Bio chondrocytes dedifferentiation follicle‐stimulating hormone IL‐6 osteoarthritis type I collagen |
| title | Follicle‐stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation |
| title_full | Follicle‐stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation |
| title_fullStr | Follicle‐stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation |
| title_full_unstemmed | Follicle‐stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation |
| title_short | Follicle‐stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation |
| title_sort | follicle stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation |
| topic | chondrocytes dedifferentiation follicle‐stimulating hormone IL‐6 osteoarthritis type I collagen |
| url | https://doi.org/10.1002/2211-5463.13238 |
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