Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria
Marinobacter is the abundant and important algal-associated and hydrocarbon biodegradation bacteria in the ocean. However, little knowledge about their phages has been reported. Here, a novel siphovirus, vB_MalS-PS3, infecting Marinobacter algicola DG893(T), was isolated from the surface waters of t...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.726074/full |
| _version_ | 1819118357647982592 |
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| author | Yundan Liu Kaiyang Zheng Baohong Liu Yantao Liang Yantao Liang Siyuan You Wenjing Zhang Xinran Zhang Yaqi Jie Hongbing Shao Hongbing Shao Yong Jiang Yong Jiang Cui Guo Cui Guo Hui He Hui He Hualong Wang Hualong Wang Yeong Yik Sung Yeong Yik Sung Wen Jye Mok Wen Jye Mok Li Lian Wong Li Lian Wong Andrew McMinn Andrew McMinn Min Wang Min Wang Min Wang |
| author_facet | Yundan Liu Kaiyang Zheng Baohong Liu Yantao Liang Yantao Liang Siyuan You Wenjing Zhang Xinran Zhang Yaqi Jie Hongbing Shao Hongbing Shao Yong Jiang Yong Jiang Cui Guo Cui Guo Hui He Hui He Hualong Wang Hualong Wang Yeong Yik Sung Yeong Yik Sung Wen Jye Mok Wen Jye Mok Li Lian Wong Li Lian Wong Andrew McMinn Andrew McMinn Min Wang Min Wang Min Wang |
| author_sort | Yundan Liu |
| collection | DOAJ |
| description | Marinobacter is the abundant and important algal-associated and hydrocarbon biodegradation bacteria in the ocean. However, little knowledge about their phages has been reported. Here, a novel siphovirus, vB_MalS-PS3, infecting Marinobacter algicola DG893(T), was isolated from the surface waters of the western Pacific Ocean. Transmission electron microscopy (TEM) indicated that vB_MalS-PS3 has the morphology of siphoviruses. VB_MalS-PS3 was stable from −20 to 55°C, and with the latent and rise periods of about 80 and 10 min, respectively. The genome sequence of VB_MalS-PS3 contains a linear, double-strand 42,168-bp DNA molecule with a G + C content of 56.23% and 54 putative open reading frames (ORFs). Nineteen conserved domains were predicted by BLASTp in NCBI. We found that vB_MalS-PS3 represent an understudied viral group with only one known isolate. The phylogenetic tree based on the amino acid sequences of whole genomes revealed that vB_MalS-PS3 has a distant evolutionary relationship with other siphoviruses, and can be grouped into a novel viral genus cluster with six uncultured assembled viral genomes from metagenomics, named here as Marinovirus. This study of the Marinobacter phage vB_MalS-PS3 genome enriched the genetic database of marine bacteriophages, in addition, will provide useful information for further research on the interaction between Marinobacter phages and their hosts, and their relationship with algal blooms and hydrocarbon biodegradation in the ocean. |
| first_indexed | 2024-12-22T05:47:36Z |
| format | Article |
| id | doaj.art-462fba47c64e4b84a73588221dc67a01 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T05:47:36Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-462fba47c64e4b84a73588221dc67a012022-12-21T18:36:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-08-011210.3389/fmicb.2021.726074726074Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated BacteriaYundan Liu0Kaiyang Zheng1Baohong Liu2Yantao Liang3Yantao Liang4Siyuan You5Wenjing Zhang6Xinran Zhang7Yaqi Jie8Hongbing Shao9Hongbing Shao10Yong Jiang11Yong Jiang12Cui Guo13Cui Guo14Hui He15Hui He16Hualong Wang17Hualong Wang18Yeong Yik Sung19Yeong Yik Sung20Wen Jye Mok21Wen Jye Mok22Li Lian Wong23Li Lian Wong24Andrew McMinn25Andrew McMinn26Min Wang27Min Wang28Min Wang29College of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaDepartment of Hospital Infection Management, Qilu Hospital, Shandong University, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaCollege of Letters and Science, University of Wisconsin–Madison, Madison, WI, United StatesCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu, MalaysiaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu, MalaysiaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu, MalaysiaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaInstitute for Marine and Antarctic Studies, University of Tasmania, Hobart, TAS, AustraliaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaUMT-OUC Joint Centre for Marine Studies, Qingdao, ChinaThe Affiliated Hospital of Qingdao University, Qingdao, ChinaMarinobacter is the abundant and important algal-associated and hydrocarbon biodegradation bacteria in the ocean. However, little knowledge about their phages has been reported. Here, a novel siphovirus, vB_MalS-PS3, infecting Marinobacter algicola DG893(T), was isolated from the surface waters of the western Pacific Ocean. Transmission electron microscopy (TEM) indicated that vB_MalS-PS3 has the morphology of siphoviruses. VB_MalS-PS3 was stable from −20 to 55°C, and with the latent and rise periods of about 80 and 10 min, respectively. The genome sequence of VB_MalS-PS3 contains a linear, double-strand 42,168-bp DNA molecule with a G + C content of 56.23% and 54 putative open reading frames (ORFs). Nineteen conserved domains were predicted by BLASTp in NCBI. We found that vB_MalS-PS3 represent an understudied viral group with only one known isolate. The phylogenetic tree based on the amino acid sequences of whole genomes revealed that vB_MalS-PS3 has a distant evolutionary relationship with other siphoviruses, and can be grouped into a novel viral genus cluster with six uncultured assembled viral genomes from metagenomics, named here as Marinovirus. This study of the Marinobacter phage vB_MalS-PS3 genome enriched the genetic database of marine bacteriophages, in addition, will provide useful information for further research on the interaction between Marinobacter phages and their hosts, and their relationship with algal blooms and hydrocarbon biodegradation in the ocean.https://www.frontiersin.org/articles/10.3389/fmicb.2021.726074/fullbacteriophageMarinobacterhydrocarbon biodegradationgenome and comparative genome analysisphylogenetic analysisalgal-associated bacteria |
| spellingShingle | Yundan Liu Kaiyang Zheng Baohong Liu Yantao Liang Yantao Liang Siyuan You Wenjing Zhang Xinran Zhang Yaqi Jie Hongbing Shao Hongbing Shao Yong Jiang Yong Jiang Cui Guo Cui Guo Hui He Hui He Hualong Wang Hualong Wang Yeong Yik Sung Yeong Yik Sung Wen Jye Mok Wen Jye Mok Li Lian Wong Li Lian Wong Andrew McMinn Andrew McMinn Min Wang Min Wang Min Wang Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria Frontiers in Microbiology bacteriophage Marinobacter hydrocarbon biodegradation genome and comparative genome analysis phylogenetic analysis algal-associated bacteria |
| title | Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria |
| title_full | Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria |
| title_fullStr | Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria |
| title_full_unstemmed | Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria |
| title_short | Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria |
| title_sort | characterization and genomic analysis of marinobacter phage vb mals ps3 representing a new lambda like temperate siphoviral genus infecting algae associated bacteria |
| topic | bacteriophage Marinobacter hydrocarbon biodegradation genome and comparative genome analysis phylogenetic analysis algal-associated bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.726074/full |
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