Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos
Assisted reproductive technologies impact transcriptome and epigenome of embryos and can result in long-term phenotypic consequences. Whole-genome DNA methylation profiles from individual bovine blastocysts in vivo- and in vitro-derived (using three sources of protein: reproductive fluids, blood ser...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6426 |
| _version_ | 1797530044509192192 |
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| author | Sebastian Canovas Elena Ivanova Meriem Hamdi Fernando Perez-Sanz Dimitrios Rizos Gavin Kelsey Pilar Coy |
| author_facet | Sebastian Canovas Elena Ivanova Meriem Hamdi Fernando Perez-Sanz Dimitrios Rizos Gavin Kelsey Pilar Coy |
| author_sort | Sebastian Canovas |
| collection | DOAJ |
| description | Assisted reproductive technologies impact transcriptome and epigenome of embryos and can result in long-term phenotypic consequences. Whole-genome DNA methylation profiles from individual bovine blastocysts in vivo- and in vitro-derived (using three sources of protein: reproductive fluids, blood serum and bovine serum albumin) were generated. The impact of in vitro culture on DNA methylation was analyzed, and sex-specific methylation differences at blastocyst stage were uncovered. In vivo embryos showed the highest levels of methylation (29.5%), close to those produced in vitro with serum, whilst embryos produced in vitro with reproductive fluids or albumin showed less global methylation (25–25.4%). During repetitive element analysis, the serum group was the most affected. DNA methylation differences between in vivo and in vitro groups were more frequent in the first intron than in CpGi in promoters. Moreover, hierarchical cluster analysis showed that sex produced a stronger bias in the results than embryo origin. For each group, distance between male and female embryos varied, with in vivo blastocyst showing a lesser distance. Between the sexually dimorphic methylated tiles, which were biased to X-chromosome, critical factors for reproduction, developmental process, cell proliferation and DNA methylation machinery were included. These results support the idea that blastocysts show sexually-dimorphic DNA methylation patterns, and the known picture about the blastocyst methylome should be reconsidered. |
| first_indexed | 2024-03-10T10:22:20Z |
| format | Article |
| id | doaj.art-46307160b7354cc48c53134f94901d52 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:22:20Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-46307160b7354cc48c53134f94901d522023-11-22T00:16:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212642610.3390/ijms22126426Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine EmbryosSebastian Canovas0Elena Ivanova1Meriem Hamdi2Fernando Perez-Sanz3Dimitrios Rizos4Gavin Kelsey5Pilar Coy6Physiology of Reproduction Group, Physiology Department, Mare Nostrum Campus, University of Murcia, 30100 Murcia, SpainEpigenetics Programme, The Babraham Institute, Cambridge CB22 3AT, UKAnimal Reproduction Department, National Institute for Agriculture and Food Research and Technology, INIA, 28040 Madrid, SpainBiomedical Research Institute of Murcia, IMIB-Arrixaca-UMU, 30120 Murcia, SpainAnimal Reproduction Department, National Institute for Agriculture and Food Research and Technology, INIA, 28040 Madrid, SpainEpigenetics Programme, The Babraham Institute, Cambridge CB22 3AT, UKPhysiology of Reproduction Group, Physiology Department, Mare Nostrum Campus, University of Murcia, 30100 Murcia, SpainAssisted reproductive technologies impact transcriptome and epigenome of embryos and can result in long-term phenotypic consequences. Whole-genome DNA methylation profiles from individual bovine blastocysts in vivo- and in vitro-derived (using three sources of protein: reproductive fluids, blood serum and bovine serum albumin) were generated. The impact of in vitro culture on DNA methylation was analyzed, and sex-specific methylation differences at blastocyst stage were uncovered. In vivo embryos showed the highest levels of methylation (29.5%), close to those produced in vitro with serum, whilst embryos produced in vitro with reproductive fluids or albumin showed less global methylation (25–25.4%). During repetitive element analysis, the serum group was the most affected. DNA methylation differences between in vivo and in vitro groups were more frequent in the first intron than in CpGi in promoters. Moreover, hierarchical cluster analysis showed that sex produced a stronger bias in the results than embryo origin. For each group, distance between male and female embryos varied, with in vivo blastocyst showing a lesser distance. Between the sexually dimorphic methylated tiles, which were biased to X-chromosome, critical factors for reproduction, developmental process, cell proliferation and DNA methylation machinery were included. These results support the idea that blastocysts show sexually-dimorphic DNA methylation patterns, and the known picture about the blastocyst methylome should be reconsidered.https://www.mdpi.com/1422-0067/22/12/6426embryoDNA methylationepigeneticpreimplantationsexual dimorphismbovine |
| spellingShingle | Sebastian Canovas Elena Ivanova Meriem Hamdi Fernando Perez-Sanz Dimitrios Rizos Gavin Kelsey Pilar Coy Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos International Journal of Molecular Sciences embryo DNA methylation epigenetic preimplantation sexual dimorphism bovine |
| title | Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos |
| title_full | Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos |
| title_fullStr | Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos |
| title_full_unstemmed | Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos |
| title_short | Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos |
| title_sort | culture medium and sex drive epigenetic reprogramming in preimplantation bovine embryos |
| topic | embryo DNA methylation epigenetic preimplantation sexual dimorphism bovine |
| url | https://www.mdpi.com/1422-0067/22/12/6426 |
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