Channelling carbon flux through the meta‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putida H
Summary Lignin‐based aromatics are attractive raw materials to derive medium‐chain length poly(3‐hydroxyalkanoates) (mcl‐PHAs), biodegradable polymers of commercial value. So far, this conversion has exclusively used the ortho‐cleavage route of Pseudomonas putida KT2440, which results in the secreti...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-11-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13705 |
| _version_ | 1818419904507805696 |
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| author | José Manuel Borrero‐de Acuña Izabook Gutierrez‐Urrutia Cristian Hidalgo‐Dumont Carla Aravena‐Carrasco Matias Orellana‐Saez Nestor Palominos‐Gonzalez Jozef B. J. H. vanDuuren Viktoria Wagner Lars Gläser Judith Becker Michael Kohlstedt Flavia C. Zacconi Christoph Wittmann Ignacio Poblete‐Castro |
| author_facet | José Manuel Borrero‐de Acuña Izabook Gutierrez‐Urrutia Cristian Hidalgo‐Dumont Carla Aravena‐Carrasco Matias Orellana‐Saez Nestor Palominos‐Gonzalez Jozef B. J. H. vanDuuren Viktoria Wagner Lars Gläser Judith Becker Michael Kohlstedt Flavia C. Zacconi Christoph Wittmann Ignacio Poblete‐Castro |
| author_sort | José Manuel Borrero‐de Acuña |
| collection | DOAJ |
| description | Summary Lignin‐based aromatics are attractive raw materials to derive medium‐chain length poly(3‐hydroxyalkanoates) (mcl‐PHAs), biodegradable polymers of commercial value. So far, this conversion has exclusively used the ortho‐cleavage route of Pseudomonas putida KT2440, which results in the secretion of toxic intermediates and limited performance. Pseudomonas putida H exhibits the ortho‐ and the meta‐cleavage pathways where the latter appears promising because it stoichiometrically yields higher levels of acetyl‐CoA. Here, we created a double‐mutant H‐ΔcatAΔA2 that utilizes the meta route exclusively and synthesized 30% more PHA on benzoate than the parental strain but suffered from catechol accumulation. The single deletion of the catA2 gene in the H strain provoked a slight attenuation on the enzymatic capacity of the ortho route (25%) and activation of the meta route by nearly 8‐fold, producing twice as much mcl‐PHAs compared to the wild type. Inline, the mutant H‐ΔcatA2 showed a 2‐fold increase in the intracellular malonyl‐CoA abundance – the main precursor for mcl‐PHAs synthesis. As inferred from flux simulation and enzyme activity assays, the superior performance of H‐ΔcatA2 benefited from reduced flux through the TCA cycle and malic enzyme and diminished by‐product formation. In a benzoate‐based fed‐batch, P. putida H‐ΔcatA2 achieved a PHA titre of 6.1 g l–1 and a volumetric productivity of 1.8 g l–1 day–1. Using Kraft lignin hydrolysate as feedstock, the engineered strain formed 1.4 g l‐ 1 PHA. The balancing of carbon flux between the parallel catechol‐degrading routes emerges as an important strategy to prevent intermediate accumulation and elevate mcl‐PHA production in P. putida H and, as shown here, sets the next level to derive this sustainable biopolymer from lignin hydrolysates and aromatics. |
| first_indexed | 2024-12-14T12:45:59Z |
| format | Article |
| id | doaj.art-4632f151d32540c9b6cdeb482b64059c |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-14T12:45:59Z |
| publishDate | 2021-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-4632f151d32540c9b6cdeb482b64059c2022-12-21T23:00:47ZengWileyMicrobial Biotechnology1751-79152021-11-011462385240210.1111/1751-7915.13705Channelling carbon flux through the meta‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putida HJosé Manuel Borrero‐de Acuña0Izabook Gutierrez‐Urrutia1Cristian Hidalgo‐Dumont2Carla Aravena‐Carrasco3Matias Orellana‐Saez4Nestor Palominos‐Gonzalez5Jozef B. J. H. vanDuuren6Viktoria Wagner7Lars Gläser8Judith Becker9Michael Kohlstedt10Flavia C. Zacconi11Christoph Wittmann12Ignacio Poblete‐Castro13Biosystems Engineering Laboratory Center for Bioinformatics and Integrative Biology (CBIB) Faculty of Life Sciences Universidad Andres Bello Santiago ChileBiosystems Engineering Laboratory Center for Bioinformatics and Integrative Biology (CBIB) Faculty of Life Sciences Universidad Andres Bello Santiago ChileBiosystems Engineering Laboratory Center for Bioinformatics and Integrative Biology (CBIB) Faculty of Life Sciences Universidad Andres Bello Santiago ChileBiosystems Engineering Laboratory Center for Bioinformatics and Integrative Biology (CBIB) Faculty of Life Sciences Universidad Andres Bello Santiago ChileBiosystems Engineering Laboratory Center for Bioinformatics and Integrative Biology (CBIB) Faculty of Life Sciences Universidad Andres Bello Santiago ChileBiosystems Engineering Laboratory Center for Bioinformatics and Integrative Biology (CBIB) Faculty of Life Sciences Universidad Andres Bello Santiago ChileInstitute of Systems Biotechnology Saarland University Saarbrücken GermanyInstitute of Systems Biotechnology Saarland University Saarbrücken GermanyInstitute of Systems Biotechnology Saarland University Saarbrücken GermanyInstitute of Systems Biotechnology Saarland University Saarbrücken GermanyInstitute of Systems Biotechnology Saarland University Saarbrücken GermanyFacultad de Química y de Farmacia Pontificia Universidad Católica de Chile Santiago ChileInstitute of Systems Biotechnology Saarland University Saarbrücken GermanyBiosystems Engineering Laboratory Center for Bioinformatics and Integrative Biology (CBIB) Faculty of Life Sciences Universidad Andres Bello Santiago ChileSummary Lignin‐based aromatics are attractive raw materials to derive medium‐chain length poly(3‐hydroxyalkanoates) (mcl‐PHAs), biodegradable polymers of commercial value. So far, this conversion has exclusively used the ortho‐cleavage route of Pseudomonas putida KT2440, which results in the secretion of toxic intermediates and limited performance. Pseudomonas putida H exhibits the ortho‐ and the meta‐cleavage pathways where the latter appears promising because it stoichiometrically yields higher levels of acetyl‐CoA. Here, we created a double‐mutant H‐ΔcatAΔA2 that utilizes the meta route exclusively and synthesized 30% more PHA on benzoate than the parental strain but suffered from catechol accumulation. The single deletion of the catA2 gene in the H strain provoked a slight attenuation on the enzymatic capacity of the ortho route (25%) and activation of the meta route by nearly 8‐fold, producing twice as much mcl‐PHAs compared to the wild type. Inline, the mutant H‐ΔcatA2 showed a 2‐fold increase in the intracellular malonyl‐CoA abundance – the main precursor for mcl‐PHAs synthesis. As inferred from flux simulation and enzyme activity assays, the superior performance of H‐ΔcatA2 benefited from reduced flux through the TCA cycle and malic enzyme and diminished by‐product formation. In a benzoate‐based fed‐batch, P. putida H‐ΔcatA2 achieved a PHA titre of 6.1 g l–1 and a volumetric productivity of 1.8 g l–1 day–1. Using Kraft lignin hydrolysate as feedstock, the engineered strain formed 1.4 g l‐ 1 PHA. The balancing of carbon flux between the parallel catechol‐degrading routes emerges as an important strategy to prevent intermediate accumulation and elevate mcl‐PHA production in P. putida H and, as shown here, sets the next level to derive this sustainable biopolymer from lignin hydrolysates and aromatics.https://doi.org/10.1111/1751-7915.13705 |
| spellingShingle | José Manuel Borrero‐de Acuña Izabook Gutierrez‐Urrutia Cristian Hidalgo‐Dumont Carla Aravena‐Carrasco Matias Orellana‐Saez Nestor Palominos‐Gonzalez Jozef B. J. H. vanDuuren Viktoria Wagner Lars Gläser Judith Becker Michael Kohlstedt Flavia C. Zacconi Christoph Wittmann Ignacio Poblete‐Castro Channelling carbon flux through the meta‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putida H Microbial Biotechnology |
| title | Channelling carbon flux through the meta‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putida H |
| title_full | Channelling carbon flux through the meta‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putida H |
| title_fullStr | Channelling carbon flux through the meta‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putida H |
| title_full_unstemmed | Channelling carbon flux through the meta‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putida H |
| title_short | Channelling carbon flux through the meta‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putida H |
| title_sort | channelling carbon flux through the meta cleavage route for improved poly 3 hydroxyalkanoate production from benzoate and lignin based aromatics in pseudomonas putida h |
| url | https://doi.org/10.1111/1751-7915.13705 |
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