Epigenetic regulation of developmental expression of Cyp2d genes in mouse liver
CYP2D6 expression in liver is age-dependent. Because epigenetic mechanisms, such as DNA methylation and histone modifications, modulate age-related gene expression during development, and are highly conserved among species, the current study examined the epigenetic regulation of age-related expressi...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-04-01
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| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383512000020 |
| _version_ | 1811302672908681216 |
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| author | Ye Li Xiao-bo Zhong |
| author_facet | Ye Li Xiao-bo Zhong |
| author_sort | Ye Li |
| collection | DOAJ |
| description | CYP2D6 expression in liver is age-dependent. Because epigenetic mechanisms, such as DNA methylation and histone modifications, modulate age-related gene expression during development, and are highly conserved among species, the current study examined the epigenetic regulation of age-related expression of the Cyp2d genes in mouse liver. DNA methylation (DNAme), histone 3 lysine 4 dimethylation (H3K4me2), and histone 3 lysine 27 trimethylation (H3K27me3) was established by ChIP-on-chip tiling microarrays from mouse livers at prenatal, neonatal, and adult stages. Levels of DNAme, H3K4me2, and H3K27me3 were analyzed in a genomic region containing the Cyp2d clustering genes and their surrounding genes. Gradually increased expression levels of the Cyp2d9, Cyp2d10, Cyp2d22, and Cyp2d26 genes from prenatal, through neonatal, to adult are associated with gradually increased levels of H3K4me2 in the nucleosomes associated with these genes. Gene expression patterns during liver development in several Cyp2d surrounding genes, such as Srebf2, Sept3, Ndufa6, Tcf2, Nfam1, and Cyb5r3, could be also explained by changes of DNA methylation, H3K4me2, or H3K27me3 in those genes. In conclusion, the current study demonstrates that the changes of DNA methylation and histone modifications are associated with age-related expression patterns of the Cyp2d genes and their surrounding genes in liver cells during development. |
| first_indexed | 2024-04-13T07:32:21Z |
| format | Article |
| id | doaj.art-46337d09e25a46ceba6ce9ed78f3deba |
| institution | Directory Open Access Journal |
| issn | 2211-3835 2211-3843 |
| language | English |
| last_indexed | 2024-04-13T07:32:21Z |
| publishDate | 2012-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-46337d09e25a46ceba6ce9ed78f3deba2022-12-22T02:56:18ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432012-04-012214615810.1016/j.apsb.2012.01.001Epigenetic regulation of developmental expression of Cyp2d genes in mouse liverYe Li0Xiao-bo Zhong1Department of Pharmacology, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, Kansas City, KS 66160, USACYP2D6 expression in liver is age-dependent. Because epigenetic mechanisms, such as DNA methylation and histone modifications, modulate age-related gene expression during development, and are highly conserved among species, the current study examined the epigenetic regulation of age-related expression of the Cyp2d genes in mouse liver. DNA methylation (DNAme), histone 3 lysine 4 dimethylation (H3K4me2), and histone 3 lysine 27 trimethylation (H3K27me3) was established by ChIP-on-chip tiling microarrays from mouse livers at prenatal, neonatal, and adult stages. Levels of DNAme, H3K4me2, and H3K27me3 were analyzed in a genomic region containing the Cyp2d clustering genes and their surrounding genes. Gradually increased expression levels of the Cyp2d9, Cyp2d10, Cyp2d22, and Cyp2d26 genes from prenatal, through neonatal, to adult are associated with gradually increased levels of H3K4me2 in the nucleosomes associated with these genes. Gene expression patterns during liver development in several Cyp2d surrounding genes, such as Srebf2, Sept3, Ndufa6, Tcf2, Nfam1, and Cyb5r3, could be also explained by changes of DNA methylation, H3K4me2, or H3K27me3 in those genes. In conclusion, the current study demonstrates that the changes of DNA methylation and histone modifications are associated with age-related expression patterns of the Cyp2d genes and their surrounding genes in liver cells during development.http://www.sciencedirect.com/science/article/pii/S2211383512000020Cyp2dDNA methylationHistone methylationLiver development |
| spellingShingle | Ye Li Xiao-bo Zhong Epigenetic regulation of developmental expression of Cyp2d genes in mouse liver Acta Pharmaceutica Sinica B Cyp2d DNA methylation Histone methylation Liver development |
| title | Epigenetic regulation of developmental expression of Cyp2d genes in mouse liver |
| title_full | Epigenetic regulation of developmental expression of Cyp2d genes in mouse liver |
| title_fullStr | Epigenetic regulation of developmental expression of Cyp2d genes in mouse liver |
| title_full_unstemmed | Epigenetic regulation of developmental expression of Cyp2d genes in mouse liver |
| title_short | Epigenetic regulation of developmental expression of Cyp2d genes in mouse liver |
| title_sort | epigenetic regulation of developmental expression of cyp2d genes in mouse liver |
| topic | Cyp2d DNA methylation Histone methylation Liver development |
| url | http://www.sciencedirect.com/science/article/pii/S2211383512000020 |
| work_keys_str_mv | AT yeli epigeneticregulationofdevelopmentalexpressionofcyp2dgenesinmouseliver AT xiaobozhong epigeneticregulationofdevelopmentalexpressionofcyp2dgenesinmouseliver |