Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclists
Background: Heavy strength (HS) and short-sprint (SS) are commonly used training methods for competitive road cyclists, with the aim to improve the anaerobic power and short time cycling performance. Knowledge of how such training methods affects biochemical as well as molecular factors, are particu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2024.1365357/full |
| _version_ | 1797265529781616640 |
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| author | Anita Ryningen Kari Rostad Elisabeth Ersvær Elisabeth Ersvær Gry Sjøholt Gøran Paulsen Gøran Paulsen Hilde Gundersen Morten Kristoffersen Lise Bjørkhaug |
| author_facet | Anita Ryningen Kari Rostad Elisabeth Ersvær Elisabeth Ersvær Gry Sjøholt Gøran Paulsen Gøran Paulsen Hilde Gundersen Morten Kristoffersen Lise Bjørkhaug |
| author_sort | Anita Ryningen |
| collection | DOAJ |
| description | Background: Heavy strength (HS) and short-sprint (SS) are commonly used training methods for competitive road cyclists, with the aim to improve the anaerobic power and short time cycling performance. Knowledge of how such training methods affects biochemical as well as molecular factors, are particularly important for determining individual recovery and long-term adaptations. The primary aim of the current study was to investigate the expression levels of small non-coding RNAs in response to HS and SS training in elite cyclists as potential biomarkers for individual optimal restitution time.Methods: Eleven well trained cyclists performed one session of HS training and one session of SS training on separate days. Blood samples were taken at baseline and 5 min, 1 h and 21 h post training. Along with physiological measurements and biochemical factors (serum creatine kinase, myoglobin, human growth hormone and plasma lactate), real-time quantitative PCR was used to explore whether HS and/or SS training influenced the abundance of 24 circulating miRNAs, in serum, associated with muscle development, angiogenesis, and/or inflammation.Results: Based on complete miRNA profiles from nine cyclists, the miRNAs showing most altered expression after both training sessions included the three striated muscle-specific miRNAs (myomiRs) miR-1-3p, 133a-3p and 133b-3p. While all three miRNAs showed significantly highest expression at 1 h post HS session, the acute effect of the SS session included a significantly higher level of miR-1-3p alone, at 5 min (highest), as well as at 1 h and 21 h post session. Correlation (negative) with biochemical markers was only shown for miR-133a-3p and CK (r = −0.786, p = 0.041) and between miR-133b-3p and [La−] (r = −0.711, p = .032), at 21 h post SS session.Conclusion: Our findings support that unique myomiRs are regulated by HS and SS training. Such knowledge may be important for individually adjusted restitution times. |
| first_indexed | 2024-04-25T00:46:15Z |
| format | Article |
| id | doaj.art-463578e1f4c44a899af471f721ae3ac3 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-25T00:46:15Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-463578e1f4c44a899af471f721ae3ac32024-03-12T04:36:07ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2024-03-011510.3389/fphys.2024.13653571365357Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclistsAnita Ryningen0Kari Rostad1Elisabeth Ersvær2Elisabeth Ersvær3Gry Sjøholt4Gøran Paulsen5Gøran Paulsen6Hilde Gundersen7Morten Kristoffersen8Lise Bjørkhaug9Department of Safety, Chemistry and Biomedical Laboratory Sciences, Western Norway University of Applied Sciences, Bergen, NorwayDepartment of Safety, Chemistry and Biomedical Laboratory Sciences, Western Norway University of Applied Sciences, Bergen, NorwayDepartment of Safety, Chemistry and Biomedical Laboratory Sciences, Western Norway University of Applied Sciences, Bergen, NorwayDepartment of Biotechnology, Inland Norway University of Applied Sciences, Lillehammer, NorwayDepartment of Safety, Chemistry and Biomedical Laboratory Sciences, Western Norway University of Applied Sciences, Bergen, NorwayDepartment of Sport, Food and Natural Sciences, Western Norway University of Applied Sciences, Sogndal, NorwayDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, NorwayDepartment of Sport and Physical Activity, Western Norway University of Applied Sciences, Bergen, NorwayDepartment of Sport and Physical Activity, Western Norway University of Applied Sciences, Bergen, NorwayDepartment of Safety, Chemistry and Biomedical Laboratory Sciences, Western Norway University of Applied Sciences, Bergen, NorwayBackground: Heavy strength (HS) and short-sprint (SS) are commonly used training methods for competitive road cyclists, with the aim to improve the anaerobic power and short time cycling performance. Knowledge of how such training methods affects biochemical as well as molecular factors, are particularly important for determining individual recovery and long-term adaptations. The primary aim of the current study was to investigate the expression levels of small non-coding RNAs in response to HS and SS training in elite cyclists as potential biomarkers for individual optimal restitution time.Methods: Eleven well trained cyclists performed one session of HS training and one session of SS training on separate days. Blood samples were taken at baseline and 5 min, 1 h and 21 h post training. Along with physiological measurements and biochemical factors (serum creatine kinase, myoglobin, human growth hormone and plasma lactate), real-time quantitative PCR was used to explore whether HS and/or SS training influenced the abundance of 24 circulating miRNAs, in serum, associated with muscle development, angiogenesis, and/or inflammation.Results: Based on complete miRNA profiles from nine cyclists, the miRNAs showing most altered expression after both training sessions included the three striated muscle-specific miRNAs (myomiRs) miR-1-3p, 133a-3p and 133b-3p. While all three miRNAs showed significantly highest expression at 1 h post HS session, the acute effect of the SS session included a significantly higher level of miR-1-3p alone, at 5 min (highest), as well as at 1 h and 21 h post session. Correlation (negative) with biochemical markers was only shown for miR-133a-3p and CK (r = −0.786, p = 0.041) and between miR-133b-3p and [La−] (r = −0.711, p = .032), at 21 h post SS session.Conclusion: Our findings support that unique myomiRs are regulated by HS and SS training. Such knowledge may be important for individually adjusted restitution times.https://www.frontiersin.org/articles/10.3389/fphys.2024.1365357/fullcyclingheavy strength trainingshort-sprint trainingMicroRNAsrecovery |
| spellingShingle | Anita Ryningen Kari Rostad Elisabeth Ersvær Elisabeth Ersvær Gry Sjøholt Gøran Paulsen Gøran Paulsen Hilde Gundersen Morten Kristoffersen Lise Bjørkhaug Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclists Frontiers in Physiology cycling heavy strength training short-sprint training MicroRNAs recovery |
| title | Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclists |
| title_full | Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclists |
| title_fullStr | Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclists |
| title_full_unstemmed | Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclists |
| title_short | Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclists |
| title_sort | acute response in circulating micrornas following a single bout of short sprint and heavy strength training in well trained cyclists |
| topic | cycling heavy strength training short-sprint training MicroRNAs recovery |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2024.1365357/full |
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