Fecal acute phase proteins in cats with chronic enteropathies
Abstract Background Chronic enteropathies (CE) are common in cats and reliable biomarkers that can distinguish different causes and predict or monitor response to treatment are currently lacking. Hypothesis To evaluate certain acute phase proteins in feces that could potentially be used as biomarker...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16802 |
| _version_ | 1797729023105695744 |
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| author | Dimitra A. Karra Chris C. Chadwick Evangelia M. Stavroulaki Matina N. Pitropaki Evgenia Flouraki Karin Allenspach Jonathan A. Lidbury Joerg M. Steiner Panagiotis G. Xenoulis |
| author_facet | Dimitra A. Karra Chris C. Chadwick Evangelia M. Stavroulaki Matina N. Pitropaki Evgenia Flouraki Karin Allenspach Jonathan A. Lidbury Joerg M. Steiner Panagiotis G. Xenoulis |
| author_sort | Dimitra A. Karra |
| collection | DOAJ |
| description | Abstract Background Chronic enteropathies (CE) are common in cats and reliable biomarkers that can distinguish different causes and predict or monitor response to treatment are currently lacking. Hypothesis To evaluate certain acute phase proteins in feces that could potentially be used as biomarkers in cats with CE. Animals Twenty‐eight cats with either inflammatory bowel disease (IBD; n = 13), food‐responsive enteropathy (FRE; n = 3) or small cell gastrointestinal lymphoma (SCGL; n = 12) and 29 healthy control cats were prospectively enrolled. Methods Fecal concentrations of haptoglobin, alpha‐1‐acid‐glycoprotein (AGP), pancreatitis‐associated protein‐1 (PAP‐1), ceruloplasmin, and C‐reactive protein (CRP) were measured using Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL) immunoassays before and after initiation of treatment. Cats were treated with diet and/or prednisolone (IBD cats), plus chlorambucil (SCGL cats). Results Compared with controls, median fecal AGP concentrations were significantly lower (25.1 vs 1.8 μg/g; P = .003) and median fecal haptoglobin (0.17 vs 0.5 μg/g), PAP‐1 (0.04 vs 0.4 μg/g) and ceruloplasmin (0.15 vs 4.2 μg/g) concentrations were significantly higher (P < .001) in cats with CE. Median fecal AGP concentrations were significantly lower (P = .01) in cats with IBD and FRE (0.6 μg/g) compared with cats with SCGL (10.75 μg/g). A significant reduction was found in CE cats after treatment for median fecal ceruloplasmin concentrations (6.36 vs 1.16 μg/g; P = .04). Conclusions Fecal AGP concentration shows promise to differentiate cats with SCGL from cats with IBD and FRE. Fecal ceruloplasmin concentrations may be useful to objectively monitor response to treatment in cats with CE. |
| first_indexed | 2024-03-12T11:22:51Z |
| format | Article |
| id | doaj.art-4636666919ac4a09a2bd5fe4e503c144 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-03-12T11:22:51Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-4636666919ac4a09a2bd5fe4e503c1442023-09-01T14:18:40ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762023-09-013751750175910.1111/jvim.16802Fecal acute phase proteins in cats with chronic enteropathiesDimitra A. Karra0Chris C. Chadwick1Evangelia M. Stavroulaki2Matina N. Pitropaki3Evgenia Flouraki4Karin Allenspach5Jonathan A. Lidbury6Joerg M. Steiner7Panagiotis G. Xenoulis8University of Thessaly Small Animal Clinic Karditsa GreeceLife Diagnostic Inc. West Chester Pennsylvania USAUniversity of Thessaly Small Animal Clinic Karditsa GreeceUniversity of Thessaly Small Animal Clinic Karditsa GreeceUniversity of Thessaly Small Animal Clinic Karditsa GreeceIowa State University College of Veterinary Medicine, Veterinary Clinical Sciences Ames Iowa USATexas A&M University – Gastrointestinal Laboratory College of Veterinary Medicine & Biomedical Sciences College Station Texas USATexas A&M University Gastrointestinal Laboratory College Station Texas USAUniversity of Thessaly Small Animal Clinic Karditsa GreeceAbstract Background Chronic enteropathies (CE) are common in cats and reliable biomarkers that can distinguish different causes and predict or monitor response to treatment are currently lacking. Hypothesis To evaluate certain acute phase proteins in feces that could potentially be used as biomarkers in cats with CE. Animals Twenty‐eight cats with either inflammatory bowel disease (IBD; n = 13), food‐responsive enteropathy (FRE; n = 3) or small cell gastrointestinal lymphoma (SCGL; n = 12) and 29 healthy control cats were prospectively enrolled. Methods Fecal concentrations of haptoglobin, alpha‐1‐acid‐glycoprotein (AGP), pancreatitis‐associated protein‐1 (PAP‐1), ceruloplasmin, and C‐reactive protein (CRP) were measured using Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL) immunoassays before and after initiation of treatment. Cats were treated with diet and/or prednisolone (IBD cats), plus chlorambucil (SCGL cats). Results Compared with controls, median fecal AGP concentrations were significantly lower (25.1 vs 1.8 μg/g; P = .003) and median fecal haptoglobin (0.17 vs 0.5 μg/g), PAP‐1 (0.04 vs 0.4 μg/g) and ceruloplasmin (0.15 vs 4.2 μg/g) concentrations were significantly higher (P < .001) in cats with CE. Median fecal AGP concentrations were significantly lower (P = .01) in cats with IBD and FRE (0.6 μg/g) compared with cats with SCGL (10.75 μg/g). A significant reduction was found in CE cats after treatment for median fecal ceruloplasmin concentrations (6.36 vs 1.16 μg/g; P = .04). Conclusions Fecal AGP concentration shows promise to differentiate cats with SCGL from cats with IBD and FRE. Fecal ceruloplasmin concentrations may be useful to objectively monitor response to treatment in cats with CE.https://doi.org/10.1111/jvim.16802AGPceruloplasminfecal biomarkershaptoglobinPAP‐1 |
| spellingShingle | Dimitra A. Karra Chris C. Chadwick Evangelia M. Stavroulaki Matina N. Pitropaki Evgenia Flouraki Karin Allenspach Jonathan A. Lidbury Joerg M. Steiner Panagiotis G. Xenoulis Fecal acute phase proteins in cats with chronic enteropathies Journal of Veterinary Internal Medicine AGP ceruloplasmin fecal biomarkers haptoglobin PAP‐1 |
| title | Fecal acute phase proteins in cats with chronic enteropathies |
| title_full | Fecal acute phase proteins in cats with chronic enteropathies |
| title_fullStr | Fecal acute phase proteins in cats with chronic enteropathies |
| title_full_unstemmed | Fecal acute phase proteins in cats with chronic enteropathies |
| title_short | Fecal acute phase proteins in cats with chronic enteropathies |
| title_sort | fecal acute phase proteins in cats with chronic enteropathies |
| topic | AGP ceruloplasmin fecal biomarkers haptoglobin PAP‐1 |
| url | https://doi.org/10.1111/jvim.16802 |
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