Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (<i>Punica granatum</i> L.) Leathery Exocarp Extract
Pomegranate (<i>Punica granatum</i> L.) fruits are important sources of vitamins and minerals and widely used in the dietary supplement industry. An aqueous extract of its leathery exocarp (LEP) was obtained by a solid-phase micro-extraction method. The antifungal activity was examined a...
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MDPI AG
2021-01-01
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| Series: | Plants |
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| author | Hazem S. Elshafie Lucia Caputo Laura De Martino Shimaa H. Sakr Vincenzo De Feo Ippolito Camele |
| author_facet | Hazem S. Elshafie Lucia Caputo Laura De Martino Shimaa H. Sakr Vincenzo De Feo Ippolito Camele |
| author_sort | Hazem S. Elshafie |
| collection | DOAJ |
| description | Pomegranate (<i>Punica granatum</i> L.) fruits are important sources of vitamins and minerals and widely used in the dietary supplement industry. An aqueous extract of its leathery exocarp (LEP) was obtained by a solid-phase micro-extraction method. The antifungal activity was examined against the phytopathogenic fungi, <i>Fusarium oxysporum</i>, <i>Phytophthora cinnamomi</i>, <i>Penicillium digitatum</i> and <i>Botrytis cinerea</i>, and the antibacterial activity was evaluated against <i>Escherichia coli</i>, <i>Xanthomonas campestris,</i><i>Bacillus megaterium</i> and <i>Clavibacter michiganensis</i>. The antimicrobial assays showed, in some cases, a promising antimicrobial effect compared to the synthetic drugs. The possible anti-acetylcholinesterase and antioxidant activities of the LEP extract were investigated by the Ellman’s assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) test, respectively, and their results showed that the LEP extract has an effective anti-acetylcholinesterase inhibitory effect and an antioxidant activity. Thus, the LEP extract could be valid as a candidate for further studies on the use of pomegranate in neurodegenerative diseases as a food preservative and a suitable substitute to control several phytopathogens. |
| first_indexed | 2024-03-09T04:51:50Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T04:51:50Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4637bd991f4b4b6c94ac6378ecddf93a2023-12-03T13:09:41ZengMDPI AGPlants2223-77472021-01-0110115310.3390/plants10010153Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (<i>Punica granatum</i> L.) Leathery Exocarp ExtractHazem S. Elshafie0Lucia Caputo1Laura De Martino2Shimaa H. Sakr3Vincenzo De Feo4Ippolito Camele5School of Agricultural, Forestry, Food and Environmental Sciences (SAFE), University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyDepartment of Pharmacy, University of Salerno, 84084 Fisciano, ItalyDepartment of Pharmacy, University of Salerno, 84084 Fisciano, ItalySchool of Agricultural, Forestry, Food and Environmental Sciences (SAFE), University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyDepartment of Pharmacy, University of Salerno, 84084 Fisciano, ItalySchool of Agricultural, Forestry, Food and Environmental Sciences (SAFE), University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyPomegranate (<i>Punica granatum</i> L.) fruits are important sources of vitamins and minerals and widely used in the dietary supplement industry. An aqueous extract of its leathery exocarp (LEP) was obtained by a solid-phase micro-extraction method. The antifungal activity was examined against the phytopathogenic fungi, <i>Fusarium oxysporum</i>, <i>Phytophthora cinnamomi</i>, <i>Penicillium digitatum</i> and <i>Botrytis cinerea</i>, and the antibacterial activity was evaluated against <i>Escherichia coli</i>, <i>Xanthomonas campestris,</i><i>Bacillus megaterium</i> and <i>Clavibacter michiganensis</i>. The antimicrobial assays showed, in some cases, a promising antimicrobial effect compared to the synthetic drugs. The possible anti-acetylcholinesterase and antioxidant activities of the LEP extract were investigated by the Ellman’s assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) test, respectively, and their results showed that the LEP extract has an effective anti-acetylcholinesterase inhibitory effect and an antioxidant activity. Thus, the LEP extract could be valid as a candidate for further studies on the use of pomegranate in neurodegenerative diseases as a food preservative and a suitable substitute to control several phytopathogens.https://www.mdpi.com/2223-7747/10/1/153plant extractsnatural productsbiocontrolplant pathogensantioxidantanti-acetylcholinesterase |
| spellingShingle | Hazem S. Elshafie Lucia Caputo Laura De Martino Shimaa H. Sakr Vincenzo De Feo Ippolito Camele Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (<i>Punica granatum</i> L.) Leathery Exocarp Extract Plants plant extracts natural products biocontrol plant pathogens antioxidant anti-acetylcholinesterase |
| title | Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (<i>Punica granatum</i> L.) Leathery Exocarp Extract |
| title_full | Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (<i>Punica granatum</i> L.) Leathery Exocarp Extract |
| title_fullStr | Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (<i>Punica granatum</i> L.) Leathery Exocarp Extract |
| title_full_unstemmed | Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (<i>Punica granatum</i> L.) Leathery Exocarp Extract |
| title_short | Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (<i>Punica granatum</i> L.) Leathery Exocarp Extract |
| title_sort | study of bio pharmaceutical and antimicrobial properties of pomegranate i punica granatum i l leathery exocarp extract |
| topic | plant extracts natural products biocontrol plant pathogens antioxidant anti-acetylcholinesterase |
| url | https://www.mdpi.com/2223-7747/10/1/153 |
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