Preparation and Biological Activity of D-Glucosamine Hydrochloride from the Waste Residues of Pleurotus eryngii
In this paper, the preparation process of D-glucosamine hydrochloride (GAH) was investigated from the waste residues of Pleurotus eryngii (P. eryngii). Liquid chromatography-mass spectrometry (HPLC-MS), infrared spectroscopy (IR), and physicochemical indexes of GAH were also studied. Then the effect...
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The editorial department of Science and Technology of Food Industry
2023-09-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110139 |
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| author | Qianru ZHANG Qici WU Yu XUE Zhichao LIN Jiafu HUANG Haokun LÜ Wei PENG Yutian PAN Jinmei LIN |
| author_facet | Qianru ZHANG Qici WU Yu XUE Zhichao LIN Jiafu HUANG Haokun LÜ Wei PENG Yutian PAN Jinmei LIN |
| author_sort | Qianru ZHANG |
| collection | DOAJ |
| description | In this paper, the preparation process of D-glucosamine hydrochloride (GAH) was investigated from the waste residues of Pleurotus eryngii (P. eryngii). Liquid chromatography-mass spectrometry (HPLC-MS), infrared spectroscopy (IR), and physicochemical indexes of GAH were also studied. Then the effect of GAH on the zebrafish embryonic development was evaluated. The experimental conditions for preparing GAH were optimized through single-factor experiments and response surface methodology. Under optimal conditions (HCl concentration of 31%, hydrolysis time of 4 h, hydrolysis temperature of 82 ℃, and the liquid-solid ratio of 5 mL/g), the yield of GAH reached 23.61%. HPLC-MS, IR, and physicochemical index analysis showed that the purity of the GAH-purified sample was 101.9% of the standard. The mass spectrometry and infrared spectroscopy were consistent with the standard. All indexes met or even outperformed the specifications of the United States Pharmacopeia 43-National Formulary 38 (USP 43-NF38), and the arsenic content was only 0.21 μg/g. In addition, the zebrafish experiment indicated that exposure to moderate and high concentrations (0.3%~0.5%) of GAH could induce more than 50% of the abnormal embryonic development of zebrafish. Moreover, 0.3% of GAH promotes the transforming growth factor-β (TGF-β) signal pathway. In conclusion, the results provide valuable insight into preparing and applying the industrial residue of P. eryngii. |
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| language | zho |
| last_indexed | 2024-03-12T13:35:18Z |
| publishDate | 2023-09-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-4639495abcd641ad91b950cd4ad805df2023-08-24T05:51:58ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-09-01441726327110.13386/j.issn1002-0306.20221101392022110139-17Preparation and Biological Activity of D-Glucosamine Hydrochloride from the Waste Residues of Pleurotus eryngiiQianru ZHANG0Qici WU1Yu XUE2Zhichao LIN3Jiafu HUANG4Haokun LÜ5Wei PENG6Yutian PAN7Jinmei LIN8Fujian Universities Engineering Research Center of Fungus Industry, Minnan Normal University, Zhangzhou 363000, ChinaFujian Universities Engineering Research Center of Fungus Industry, Minnan Normal University, Zhangzhou 363000, ChinaFujian Universities Engineering Research Center of Fungus Industry, Minnan Normal University, Zhangzhou 363000, ChinaFujian Universities Engineering Research Center of Fungus Industry, Minnan Normal University, Zhangzhou 363000, ChinaFujian Universities Engineering Research Center of Fungus Industry, Minnan Normal University, Zhangzhou 363000, ChinaFujian Universities Engineering Research Center of Fungus Industry, Minnan Normal University, Zhangzhou 363000, ChinaFujian Universities Engineering Research Center of Fungus Industry, Minnan Normal University, Zhangzhou 363000, ChinaFujian Universities Engineering Research Center of Fungus Industry, Minnan Normal University, Zhangzhou 363000, ChinaCollege of Chemistry and Environment, Minnan Normal University, Zhangzhou 363000, ChinaIn this paper, the preparation process of D-glucosamine hydrochloride (GAH) was investigated from the waste residues of Pleurotus eryngii (P. eryngii). Liquid chromatography-mass spectrometry (HPLC-MS), infrared spectroscopy (IR), and physicochemical indexes of GAH were also studied. Then the effect of GAH on the zebrafish embryonic development was evaluated. The experimental conditions for preparing GAH were optimized through single-factor experiments and response surface methodology. Under optimal conditions (HCl concentration of 31%, hydrolysis time of 4 h, hydrolysis temperature of 82 ℃, and the liquid-solid ratio of 5 mL/g), the yield of GAH reached 23.61%. HPLC-MS, IR, and physicochemical index analysis showed that the purity of the GAH-purified sample was 101.9% of the standard. The mass spectrometry and infrared spectroscopy were consistent with the standard. All indexes met or even outperformed the specifications of the United States Pharmacopeia 43-National Formulary 38 (USP 43-NF38), and the arsenic content was only 0.21 μg/g. In addition, the zebrafish experiment indicated that exposure to moderate and high concentrations (0.3%~0.5%) of GAH could induce more than 50% of the abnormal embryonic development of zebrafish. Moreover, 0.3% of GAH promotes the transforming growth factor-β (TGF-β) signal pathway. In conclusion, the results provide valuable insight into preparing and applying the industrial residue of P. eryngii.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110139d-glucosamine hydrochloridepleurotus eryngiiwaste residuesresponse surface optimizationzebrafish embryonic development |
| spellingShingle | Qianru ZHANG Qici WU Yu XUE Zhichao LIN Jiafu HUANG Haokun LÜ Wei PENG Yutian PAN Jinmei LIN Preparation and Biological Activity of D-Glucosamine Hydrochloride from the Waste Residues of Pleurotus eryngii Shipin gongye ke-ji d-glucosamine hydrochloride pleurotus eryngii waste residues response surface optimization zebrafish embryonic development |
| title | Preparation and Biological Activity of D-Glucosamine Hydrochloride from the Waste Residues of Pleurotus eryngii |
| title_full | Preparation and Biological Activity of D-Glucosamine Hydrochloride from the Waste Residues of Pleurotus eryngii |
| title_fullStr | Preparation and Biological Activity of D-Glucosamine Hydrochloride from the Waste Residues of Pleurotus eryngii |
| title_full_unstemmed | Preparation and Biological Activity of D-Glucosamine Hydrochloride from the Waste Residues of Pleurotus eryngii |
| title_short | Preparation and Biological Activity of D-Glucosamine Hydrochloride from the Waste Residues of Pleurotus eryngii |
| title_sort | preparation and biological activity of d glucosamine hydrochloride from the waste residues of pleurotus eryngii |
| topic | d-glucosamine hydrochloride pleurotus eryngii waste residues response surface optimization zebrafish embryonic development |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110139 |
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