Protein residue network analysis reveals fundamental properties of the human coagulation factor VIII
Abstract Hemophilia A is an X-linked inherited blood coagulation disorder caused by the production and circulation of defective coagulation factor VIII protein. People living with this condition receive either prophylaxis or on-demand treatment, and approximately 30% of patients develop inhibitor an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-92201-3 |
| _version_ | 1818722525020946432 |
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| author | Tiago J. S. Lopes Ricardo Rios Tatiane Nogueira Rodrigo F. Mello |
| author_facet | Tiago J. S. Lopes Ricardo Rios Tatiane Nogueira Rodrigo F. Mello |
| author_sort | Tiago J. S. Lopes |
| collection | DOAJ |
| description | Abstract Hemophilia A is an X-linked inherited blood coagulation disorder caused by the production and circulation of defective coagulation factor VIII protein. People living with this condition receive either prophylaxis or on-demand treatment, and approximately 30% of patients develop inhibitor antibodies, a serious complication that limits treatment options. Although previous studies performed targeted mutations to identify important residues of FVIII, a detailed understanding of the role of each amino acid and their neighboring residues is still lacking. Here, we addressed this issue by creating a residue interaction network (RIN) where the nodes are the FVIII residues, and two nodes are connected if their corresponding residues are in close proximity in the FVIII protein structure. We studied the characteristics of all residues in this network and found important properties related to disease severity, interaction to other proteins and structural stability. Importantly, we found that the RIN-derived properties were in close agreement with in vitro and clinical reports, corroborating the observation that the patterns derived from this detailed map of the FVIII protein architecture accurately capture the biological properties of FVIII. |
| first_indexed | 2024-12-17T20:56:00Z |
| format | Article |
| id | doaj.art-463b9a2b09e6452aa5dfb631fb3f159b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T20:56:00Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-463b9a2b09e6452aa5dfb631fb3f159b2022-12-21T21:32:53ZengNature PortfolioScientific Reports2045-23222021-06-0111111110.1038/s41598-021-92201-3Protein residue network analysis reveals fundamental properties of the human coagulation factor VIIITiago J. S. Lopes0Ricardo Rios1Tatiane Nogueira2Rodrigo F. Mello3Department of Reproductive Biology, Center for Regenerative Medicine, National Center for Child Health and Development Research InstituteDepartment of Computer Science, Federal University of BahiaDepartment of Computer Science, Federal University of BahiaInstitute of Mathematics and Computer Science, University of São PauloAbstract Hemophilia A is an X-linked inherited blood coagulation disorder caused by the production and circulation of defective coagulation factor VIII protein. People living with this condition receive either prophylaxis or on-demand treatment, and approximately 30% of patients develop inhibitor antibodies, a serious complication that limits treatment options. Although previous studies performed targeted mutations to identify important residues of FVIII, a detailed understanding of the role of each amino acid and their neighboring residues is still lacking. Here, we addressed this issue by creating a residue interaction network (RIN) where the nodes are the FVIII residues, and two nodes are connected if their corresponding residues are in close proximity in the FVIII protein structure. We studied the characteristics of all residues in this network and found important properties related to disease severity, interaction to other proteins and structural stability. Importantly, we found that the RIN-derived properties were in close agreement with in vitro and clinical reports, corroborating the observation that the patterns derived from this detailed map of the FVIII protein architecture accurately capture the biological properties of FVIII.https://doi.org/10.1038/s41598-021-92201-3 |
| spellingShingle | Tiago J. S. Lopes Ricardo Rios Tatiane Nogueira Rodrigo F. Mello Protein residue network analysis reveals fundamental properties of the human coagulation factor VIII Scientific Reports |
| title | Protein residue network analysis reveals fundamental properties of the human coagulation factor VIII |
| title_full | Protein residue network analysis reveals fundamental properties of the human coagulation factor VIII |
| title_fullStr | Protein residue network analysis reveals fundamental properties of the human coagulation factor VIII |
| title_full_unstemmed | Protein residue network analysis reveals fundamental properties of the human coagulation factor VIII |
| title_short | Protein residue network analysis reveals fundamental properties of the human coagulation factor VIII |
| title_sort | protein residue network analysis reveals fundamental properties of the human coagulation factor viii |
| url | https://doi.org/10.1038/s41598-021-92201-3 |
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