miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1
Abstract Background The aberrant expression of microRNA-140-5p (miR-140-5p) has been described in gastric cancer (GC). However, the role of miR-140-5p in GC remains unclear. In this study, the prognostic relevance of miR-140-5p in GC was investigated and YES1 was identified as a novel target of miR-...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Molecular Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12943-017-0708-6 |
| _version_ | 1818437853889167360 |
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| author | Zheng Fang Shuai Yin Ruochuan Sun Shangxin Zhang Min Fu Youliang Wu Tao Zhang Junaid Khaliq Yongxiang Li |
| author_facet | Zheng Fang Shuai Yin Ruochuan Sun Shangxin Zhang Min Fu Youliang Wu Tao Zhang Junaid Khaliq Yongxiang Li |
| author_sort | Zheng Fang |
| collection | DOAJ |
| description | Abstract Background The aberrant expression of microRNA-140-5p (miR-140-5p) has been described in gastric cancer (GC). However, the role of miR-140-5p in GC remains unclear. In this study, the prognostic relevance of miR-140-5p in GC was investigated and YES1 was identified as a novel target of miR-140-5p in regulating tumor progression. Methods miR-140-5p level was determined in 20 paired frozen specimens through quantitative real-time PCR, and analyzed in tissue microarrays through in situ hybridization. The target of miR-140-5p was verified through a dual luciferase reporter assay, and the effects of miR-140-5p on phenotypic changes in GC cells were investigated in vitro and in vivo. Results Compared with that in adjacent normal tissues, miR-140-5p expression decreased in cancerous tissues. The downregulated miR-140-5p in 144 patients with GC was significantly correlated with the reduced overall survival of these patients. miR-140-5p could inhibit GC cell proliferation, migration and invasion by directly targeting 3′–untranlated region of YES1. miR-140-5p could also remarkably reduce the tumor size in GC xenograft mice. Conclusions miR-140-5p serves as a potential prognostic factor in patients with GC, and miR-140-5p mediated YES1 inhibition is a novel mechanism behind the suppressive effects of miR-140-5p in GC. |
| first_indexed | 2024-12-14T17:31:17Z |
| format | Article |
| id | doaj.art-463d940e7cd44dbfb5597f615b5e04e9 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-14T17:31:17Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-463d940e7cd44dbfb5597f615b5e04e92022-12-21T22:53:05ZengBMCMolecular Cancer1476-45982017-08-0116111110.1186/s12943-017-0708-6miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1Zheng Fang0Shuai Yin1Ruochuan Sun2Shangxin Zhang3Min Fu4Youliang Wu5Tao Zhang6Junaid Khaliq7Yongxiang Li8Department of General Surgery, First affiliated Hospital of Anhui Medical UniversityDepartment of General, Visceral, Transplantation, Vascular and Thoracic Surgery, Hospital of University of MunichDepartment of General Surgery, First affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, First affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, First affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, First affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, First affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, First affiliated Hospital of Anhui Medical UniversityDepartment of General Surgery, First affiliated Hospital of Anhui Medical UniversityAbstract Background The aberrant expression of microRNA-140-5p (miR-140-5p) has been described in gastric cancer (GC). However, the role of miR-140-5p in GC remains unclear. In this study, the prognostic relevance of miR-140-5p in GC was investigated and YES1 was identified as a novel target of miR-140-5p in regulating tumor progression. Methods miR-140-5p level was determined in 20 paired frozen specimens through quantitative real-time PCR, and analyzed in tissue microarrays through in situ hybridization. The target of miR-140-5p was verified through a dual luciferase reporter assay, and the effects of miR-140-5p on phenotypic changes in GC cells were investigated in vitro and in vivo. Results Compared with that in adjacent normal tissues, miR-140-5p expression decreased in cancerous tissues. The downregulated miR-140-5p in 144 patients with GC was significantly correlated with the reduced overall survival of these patients. miR-140-5p could inhibit GC cell proliferation, migration and invasion by directly targeting 3′–untranlated region of YES1. miR-140-5p could also remarkably reduce the tumor size in GC xenograft mice. Conclusions miR-140-5p serves as a potential prognostic factor in patients with GC, and miR-140-5p mediated YES1 inhibition is a novel mechanism behind the suppressive effects of miR-140-5p in GC.http://link.springer.com/article/10.1186/s12943-017-0708-6miR-140-5pgastric cancerprognostic factorYES1tumor progression |
| spellingShingle | Zheng Fang Shuai Yin Ruochuan Sun Shangxin Zhang Min Fu Youliang Wu Tao Zhang Junaid Khaliq Yongxiang Li miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1 Molecular Cancer miR-140-5p gastric cancer prognostic factor YES1 tumor progression |
| title | miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1 |
| title_full | miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1 |
| title_fullStr | miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1 |
| title_full_unstemmed | miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1 |
| title_short | miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1 |
| title_sort | mir 140 5p suppresses the proliferation migration and invasion of gastric cancer by regulating yes1 |
| topic | miR-140-5p gastric cancer prognostic factor YES1 tumor progression |
| url | http://link.springer.com/article/10.1186/s12943-017-0708-6 |
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