Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish
Summary: In this protocol, we describe steps that utilize the optical clarity of the zebrafish larvae and the stereotyped motor neuron axon structure in the trunk to measure spontaneous or evoked motor neuron axon activity. This activity is detected with transgenic fluorescent indicators introduced...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006463 |
| _version_ | 1811199602422972416 |
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| author | Hiu-tung Candy Wong Catherine M. Drerup |
| author_facet | Hiu-tung Candy Wong Catherine M. Drerup |
| author_sort | Hiu-tung Candy Wong |
| collection | DOAJ |
| description | Summary: In this protocol, we describe steps that utilize the optical clarity of the zebrafish larvae and the stereotyped motor neuron axon structure in the trunk to measure spontaneous or evoked motor neuron axon activity. This activity is detected with transgenic fluorescent indicators introduced into the larvae by zygotic injection. Fluorescent indicator intensity changes in the small neuromuscular junctions are quantified to measure the presynaptic calcium activity and consequent synaptic vesicle release.For complete details on the use and execution of this protocol, please refer to Mandal et al. (2020). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-12T01:51:28Z |
| format | Article |
| id | doaj.art-46436a2e02374d13817c3a0321aeedda |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-12T01:51:28Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-46436a2e02374d13817c3a0321aeedda2022-12-22T03:52:56ZengElsevierSTAR Protocols2666-16672022-12-0134101766Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafishHiu-tung Candy Wong0Catherine M. Drerup1Department of Integrative Biology, University of Wisconsin-Madison, Madison, WI 53706, USA; Corresponding authorDepartment of Integrative Biology, University of Wisconsin-Madison, Madison, WI 53706, USA; Corresponding authorSummary: In this protocol, we describe steps that utilize the optical clarity of the zebrafish larvae and the stereotyped motor neuron axon structure in the trunk to measure spontaneous or evoked motor neuron axon activity. This activity is detected with transgenic fluorescent indicators introduced into the larvae by zygotic injection. Fluorescent indicator intensity changes in the small neuromuscular junctions are quantified to measure the presynaptic calcium activity and consequent synaptic vesicle release.For complete details on the use and execution of this protocol, please refer to Mandal et al. (2020). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006463MicroscopyModel OrganismsNeuroscienceMolecular/Chemical Probes |
| spellingShingle | Hiu-tung Candy Wong Catherine M. Drerup Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish STAR Protocols Microscopy Model Organisms Neuroscience Molecular/Chemical Probes |
| title | Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish |
| title_full | Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish |
| title_fullStr | Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish |
| title_full_unstemmed | Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish |
| title_short | Using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish |
| title_sort | using fluorescent indicators for in vivo quantification of spontaneous or evoked motor neuron presynaptic activity in transgenic zebrafish |
| topic | Microscopy Model Organisms Neuroscience Molecular/Chemical Probes |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006463 |
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