Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves
Context: Carica papaya Linn. (Caricaceae) leaf (CPL) juice has long been traditionally used in ethnomedicine for dengue fever. Objective: The study examines the effects of standardized CPL aqueous extract (SCPLE) on platelet count, extramedullary haematopoiesis (EMH), and immunomodulation in cycloph...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2017.1346690 |
| _version_ | 1818249075652296704 |
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| author | Varisha Anjum Poonam Arora Shahid Husain Ansari Abul Kalam Najmi Sayeed Ahmad |
| author_facet | Varisha Anjum Poonam Arora Shahid Husain Ansari Abul Kalam Najmi Sayeed Ahmad |
| author_sort | Varisha Anjum |
| collection | DOAJ |
| description | Context: Carica papaya Linn. (Caricaceae) leaf (CPL) juice has long been traditionally used in ethnomedicine for dengue fever. Objective: The study examines the effects of standardized CPL aqueous extract (SCPLE) on platelet count, extramedullary haematopoiesis (EMH), and immunomodulation in cyclophosphamide (CP)-induced animal model of thrombocytopenia. Materials and methods: The extract was analyzed for myricetin, caffeic acid, trans-ferulic acid, and kaempferol using HPTLC for standardization followed by UPLC-qTOF/MS fingerprinting for metabolite signature. The effects of SCPLE (50 and 150 mg/kg p.o.) on proliferative response of platelet count and total leucocyte count (TLC) were observed up to 14 days in Wistar rat. However, delayed-type hypersensitivity (DTH), haemagglutination titre (HT), and in vivo carbon clearance were examined as immunomodulatory parameters in albino mice at 150 mg/kg p.o. against CP. Results: The quantitative HPTLC estimation of SCPLE showed the presence of myricetin, caffeic acid, trans-ferulic acid, and kaempferol up to 280.16 ± 5.99, 370.18 ± 6.27, 1110.86 ± 2.97, and 160.53 ± 2.48 (μg/g), respectively. Twenty-four metabolites were identified using UPLC-qTOF/MS. Oral administration of SCPLE (150 mg/kg) in thrombocytopenic rats exhibited significant (p < 0.01) increase in thrombocytes (1014.83 × 103 cells/mm3), DTH response (0.16 ± 0.004), and phagocytic index (63.15% increase) as compared to CP-induced thrombocytopenia group. Histopathological studies showed minimal fibrosis in spleen histology. Discussion and conclusions: Results suggest CPL can mediate the release of platelets providing the means for the treatment and prevention of dengue. |
| first_indexed | 2024-12-12T15:30:44Z |
| format | Article |
| id | doaj.art-4643b57a201c4639a01901315915c2b9 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-12T15:30:44Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-4643b57a201c4639a01901315915c2b92022-12-22T00:20:08ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015512043205610.1080/13880209.2017.13466901346690Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leavesVarisha Anjum0Poonam Arora1Shahid Husain Ansari2Abul Kalam Najmi3Sayeed Ahmad4Jamia Hamdard UniversityJamia Hamdard UniversityJamia Hamdard UniversityJamia Hamdard UniversityJamia Hamdard UniversityContext: Carica papaya Linn. (Caricaceae) leaf (CPL) juice has long been traditionally used in ethnomedicine for dengue fever. Objective: The study examines the effects of standardized CPL aqueous extract (SCPLE) on platelet count, extramedullary haematopoiesis (EMH), and immunomodulation in cyclophosphamide (CP)-induced animal model of thrombocytopenia. Materials and methods: The extract was analyzed for myricetin, caffeic acid, trans-ferulic acid, and kaempferol using HPTLC for standardization followed by UPLC-qTOF/MS fingerprinting for metabolite signature. The effects of SCPLE (50 and 150 mg/kg p.o.) on proliferative response of platelet count and total leucocyte count (TLC) were observed up to 14 days in Wistar rat. However, delayed-type hypersensitivity (DTH), haemagglutination titre (HT), and in vivo carbon clearance were examined as immunomodulatory parameters in albino mice at 150 mg/kg p.o. against CP. Results: The quantitative HPTLC estimation of SCPLE showed the presence of myricetin, caffeic acid, trans-ferulic acid, and kaempferol up to 280.16 ± 5.99, 370.18 ± 6.27, 1110.86 ± 2.97, and 160.53 ± 2.48 (μg/g), respectively. Twenty-four metabolites were identified using UPLC-qTOF/MS. Oral administration of SCPLE (150 mg/kg) in thrombocytopenic rats exhibited significant (p < 0.01) increase in thrombocytes (1014.83 × 103 cells/mm3), DTH response (0.16 ± 0.004), and phagocytic index (63.15% increase) as compared to CP-induced thrombocytopenia group. Histopathological studies showed minimal fibrosis in spleen histology. Discussion and conclusions: Results suggest CPL can mediate the release of platelets providing the means for the treatment and prevention of dengue.http://dx.doi.org/10.1080/13880209.2017.1346690dengue feverthrombocytopeniaimmunomodulationuplc-qtof/ms |
| spellingShingle | Varisha Anjum Poonam Arora Shahid Husain Ansari Abul Kalam Najmi Sayeed Ahmad Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves Pharmaceutical Biology dengue fever thrombocytopenia immunomodulation uplc-qtof/ms |
| title | Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves |
| title_full | Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves |
| title_fullStr | Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves |
| title_full_unstemmed | Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves |
| title_short | Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves |
| title_sort | antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of carica papaya leaves |
| topic | dengue fever thrombocytopenia immunomodulation uplc-qtof/ms |
| url | http://dx.doi.org/10.1080/13880209.2017.1346690 |
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