The Diagnosis Significance of Serum Cysteine Protease Inhibitors (CST4) in Colorectal Cancer
Objective This study aims to evaluate the diagnostic value of human serum cysteine protease inhibitors (cystatin 4 [CST4]) in colorectal cancer (CRC) patients. Methods A total of 291 patients who were admitted to Zhuzhou Central Hospital for colonoscopy from January 2020 to December 2021 and met the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-04-01
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| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338231164232 |
| _version_ | 1827959322531856384 |
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| author | Meiyuan Huang BS Zhenyu Yang MS Jianqiang Ren MS Taoli Wang BS Dongliang Chen BS Yanqiong Zhan BS Wenxing Le MS Shuang Deng BS |
| author_facet | Meiyuan Huang BS Zhenyu Yang MS Jianqiang Ren MS Taoli Wang BS Dongliang Chen BS Yanqiong Zhan BS Wenxing Le MS Shuang Deng BS |
| author_sort | Meiyuan Huang BS |
| collection | DOAJ |
| description | Objective This study aims to evaluate the diagnostic value of human serum cysteine protease inhibitors (cystatin 4 [CST4]) in colorectal cancer (CRC) patients. Methods A total of 291 patients who were admitted to Zhuzhou Central Hospital for colonoscopy from January 2020 to December 2021 and met the inclusion criteria were selected. Serum samples of the patients were collected, and CST4 was detected by double-antibody sandwich enzyme-linked immunosorbent assay. Simultaneously, CEA and CA19-9 were detected, and the patients were divided into the CRC group, benign lesion group, and healthy control group. An attempt was made to construct a CRC prediction model including CST4 and draw a subject working characteristic curve as a diagnostic threshold for CRC prediction, and evaluate the diagnostic efficacy of the above indicators. At the same time, the expression analysis of CST4, CEA, and CA19-9 was verified by combining the data of CRC in the Tumor Genome Atlas (TCGA). Results In this study, the levels of serum CST4, CEA, and CA19-9 in the CRC group were higher than those in the colorectal benign lesion group and healthy control group, with statistical significance ( P < .001). The analysis results of the receiver operating characteristic curve showed that the area under the receiver operator characteristic curve (AUC) of CST4 was 0.7739, which was obviously larger than the AUC of CA19-9 and CEA. CRC data from the TCGA expression database showed that CST4 expression and CEA expression were higher in CRC patients than in normal samples. The combined model based on CST4 was successfully constructed, and the AUC for predicting the occurrence of CRC was 0.7851. Conclusion CST4 is a novel and improved diagnostic marker for CRC. The combined model based on CST4 has a certain potential value in terms of predicting the occurrence of intestinal cancer. |
| first_indexed | 2024-04-09T15:50:39Z |
| format | Article |
| id | doaj.art-4643d0c8d3ef4f7e8efaa640c3b156ae |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-04-09T15:50:39Z |
| publishDate | 2023-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-4643d0c8d3ef4f7e8efaa640c3b156ae2023-04-26T09:03:35ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382023-04-012210.1177/15330338231164232The Diagnosis Significance of Serum Cysteine Protease Inhibitors (CST4) in Colorectal CancerMeiyuan Huang BS0Zhenyu Yang MS1Jianqiang Ren MS2Taoli Wang BS3Dongliang Chen BS4Yanqiong Zhan BS5Wenxing Le MS6Shuang Deng BS7 Department of Pathology, Zhuzhou Central Hospital, Zhuzhou, Hunan, China Department of Pathology, Zhuzhou Central Hospital, Zhuzhou, Hunan, China Department of Pathology, Zhuzhou Central Hospital, Zhuzhou, Hunan, China Department of Pathology, Zhuzhou Central Hospital, Zhuzhou, Hunan, China Department of Pathology, Zhuzhou Central Hospital, Zhuzhou, Hunan, China Digestive Endoscopy Center, Zhuzhou Central Hospital, Zhuzhou, Hunan, China Department of Pathology, Zhuzhou Central Hospital, Zhuzhou, Hunan, China Department of Pathology, Zhuzhou Central Hospital, Zhuzhou, Hunan, ChinaObjective This study aims to evaluate the diagnostic value of human serum cysteine protease inhibitors (cystatin 4 [CST4]) in colorectal cancer (CRC) patients. Methods A total of 291 patients who were admitted to Zhuzhou Central Hospital for colonoscopy from January 2020 to December 2021 and met the inclusion criteria were selected. Serum samples of the patients were collected, and CST4 was detected by double-antibody sandwich enzyme-linked immunosorbent assay. Simultaneously, CEA and CA19-9 were detected, and the patients were divided into the CRC group, benign lesion group, and healthy control group. An attempt was made to construct a CRC prediction model including CST4 and draw a subject working characteristic curve as a diagnostic threshold for CRC prediction, and evaluate the diagnostic efficacy of the above indicators. At the same time, the expression analysis of CST4, CEA, and CA19-9 was verified by combining the data of CRC in the Tumor Genome Atlas (TCGA). Results In this study, the levels of serum CST4, CEA, and CA19-9 in the CRC group were higher than those in the colorectal benign lesion group and healthy control group, with statistical significance ( P < .001). The analysis results of the receiver operating characteristic curve showed that the area under the receiver operator characteristic curve (AUC) of CST4 was 0.7739, which was obviously larger than the AUC of CA19-9 and CEA. CRC data from the TCGA expression database showed that CST4 expression and CEA expression were higher in CRC patients than in normal samples. The combined model based on CST4 was successfully constructed, and the AUC for predicting the occurrence of CRC was 0.7851. Conclusion CST4 is a novel and improved diagnostic marker for CRC. The combined model based on CST4 has a certain potential value in terms of predicting the occurrence of intestinal cancer.https://doi.org/10.1177/15330338231164232 |
| spellingShingle | Meiyuan Huang BS Zhenyu Yang MS Jianqiang Ren MS Taoli Wang BS Dongliang Chen BS Yanqiong Zhan BS Wenxing Le MS Shuang Deng BS The Diagnosis Significance of Serum Cysteine Protease Inhibitors (CST4) in Colorectal Cancer Technology in Cancer Research & Treatment |
| title | The Diagnosis Significance of Serum
Cysteine Protease Inhibitors (CST4) in Colorectal Cancer |
| title_full | The Diagnosis Significance of Serum
Cysteine Protease Inhibitors (CST4) in Colorectal Cancer |
| title_fullStr | The Diagnosis Significance of Serum
Cysteine Protease Inhibitors (CST4) in Colorectal Cancer |
| title_full_unstemmed | The Diagnosis Significance of Serum
Cysteine Protease Inhibitors (CST4) in Colorectal Cancer |
| title_short | The Diagnosis Significance of Serum
Cysteine Protease Inhibitors (CST4) in Colorectal Cancer |
| title_sort | diagnosis significance of serum cysteine protease inhibitors cst4 in colorectal cancer |
| url | https://doi.org/10.1177/15330338231164232 |
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