Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity
Background: Asthma is a heterogeneous disorder of the airways related to inflammation; it affects millions of people worldwide. Due to the side effects of inhaled corticosteroids, researchers focused on the therapeutic effects of compounds derived from natural products. Objective: To investigate t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2022-12-01
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| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | https://iji.sums.ac.ir/article_48947_361e72e7b4e491e6b9fad160102f4931.pdf |
| _version_ | 1811177145971507200 |
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| author | Xiaolei Shi Lina Zhao Liyan Niu Jhao Wei Xuwen Li Yongri Jin |
| author_facet | Xiaolei Shi Lina Zhao Liyan Niu Jhao Wei Xuwen Li Yongri Jin |
| author_sort | Xiaolei Shi |
| collection | DOAJ |
| description | Background: Asthma is a heterogeneous disorder of the airways related to inflammation; it affects millions of people worldwide. Due to the side effects of inhaled corticosteroids, researchers focused on the therapeutic effects of compounds derived from natural products. Objective: To investigate the therapeutic benefits of Narirutin a valuable flavonoid in Citri Reticulatae Pericarpium for asthma. Methods: Narirutin was extracted using the enzyme-assisted method with the L9 (34) orthogonal array to optimize the temperatures, pH, and reaction time. The mechanism of action of Narirutin was investigated via ELISA, flow cytometry, and Western blot analysis in vivo. Results: Narirutin suppressed inflammatory cell infiltration in the lung tissue and decreased IgE and IgG1 levels in serum in vivo. It can also alleviate interleukin (IL)-4, IL-5, and interferon-γ concentrations in bronchoalveolar lavage fluid in mice. Moreover, it increased the ratio of CD4+/CD8+ T cells. Additionally, Narirutin significantly suppressed p-ERK1/2 and p-JNK expression in the MAPK signaling pathway. Conclusion: Narirutin affects the Th1/Th2 imbalance through the p-ERK and p-JNK suppression in the MAPK signaling pathway. |
| first_indexed | 2024-04-10T22:57:53Z |
| format | Article |
| id | doaj.art-46443183ea5e4e4bb3439e1ecd2e813e |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-04-10T22:57:53Z |
| publishDate | 2022-12-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-46443183ea5e4e4bb3439e1ecd2e813e2023-01-14T06:05:51ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2022-12-0119438539410.22034/iji.2022.91640.209548947Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma ActivityXiaolei Shi0Lina Zhao1Liyan Niu2Jhao Wei3Xuwen Li4Yongri Jin5College of Food Science and Engineering, Jilin University, Changchun, ChinaCollege of Food Science and Engineering, Jilin University, Changchun, ChinaCollege of Food Science and Engineering, Jilin University, Changchun, ChinaCollege of Food Science and Engineering, Jilin University, Changchun, ChinaCollege of Chemistry, Jilin University, Changchun, ChinaCollege of Chemistry, Jilin University, Changchun, ChinaBackground: Asthma is a heterogeneous disorder of the airways related to inflammation; it affects millions of people worldwide. Due to the side effects of inhaled corticosteroids, researchers focused on the therapeutic effects of compounds derived from natural products. Objective: To investigate the therapeutic benefits of Narirutin a valuable flavonoid in Citri Reticulatae Pericarpium for asthma. Methods: Narirutin was extracted using the enzyme-assisted method with the L9 (34) orthogonal array to optimize the temperatures, pH, and reaction time. The mechanism of action of Narirutin was investigated via ELISA, flow cytometry, and Western blot analysis in vivo. Results: Narirutin suppressed inflammatory cell infiltration in the lung tissue and decreased IgE and IgG1 levels in serum in vivo. It can also alleviate interleukin (IL)-4, IL-5, and interferon-γ concentrations in bronchoalveolar lavage fluid in mice. Moreover, it increased the ratio of CD4+/CD8+ T cells. Additionally, Narirutin significantly suppressed p-ERK1/2 and p-JNK expression in the MAPK signaling pathway. Conclusion: Narirutin affects the Th1/Th2 imbalance through the p-ERK and p-JNK suppression in the MAPK signaling pathway.https://iji.sums.ac.ir/article_48947_361e72e7b4e491e6b9fad160102f4931.pdfallergycitri reticulatae pericarpiumimmunologyinflammationnarirutin |
| spellingShingle | Xiaolei Shi Lina Zhao Liyan Niu Jhao Wei Xuwen Li Yongri Jin Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity Iranian Journal of Immunology allergy citri reticulatae pericarpium immunology inflammation narirutin |
| title | Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity |
| title_full | Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity |
| title_fullStr | Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity |
| title_full_unstemmed | Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity |
| title_short | Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity |
| title_sort | enzyme assisted extraction of narirutin from citri reticulatae pericarpium and anti allergic asthma activity |
| topic | allergy citri reticulatae pericarpium immunology inflammation narirutin |
| url | https://iji.sums.ac.ir/article_48947_361e72e7b4e491e6b9fad160102f4931.pdf |
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