The Glutathione <i>S</i>-Transferase <i>PtGSTF1</i> Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar
Glutathione <i>S</i>-transferases (GSTs) play an essential role in plant cell detoxification and secondary metabolism. However, their accurate functions in the growth and response to abiotic stress in woody plants are still largely unknown. In this work, a Phi class Glutathione <i>...
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2022-09-01
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| author | Hongsheng Gao Chunyan Yu Ruichao Liu Xiaoyan Li Huiqing Huang Xueting Wang Chao Zhang Ning Jiang Xiaofang Li Shuang Cheng Hongxia Zhang Bei Li |
| author_facet | Hongsheng Gao Chunyan Yu Ruichao Liu Xiaoyan Li Huiqing Huang Xueting Wang Chao Zhang Ning Jiang Xiaofang Li Shuang Cheng Hongxia Zhang Bei Li |
| author_sort | Hongsheng Gao |
| collection | DOAJ |
| description | Glutathione <i>S</i>-transferases (GSTs) play an essential role in plant cell detoxification and secondary metabolism. However, their accurate functions in the growth and response to abiotic stress in woody plants are still largely unknown. In this work, a Phi class Glutathione <i>S</i>-transferase encoding gene <i>PtGSTF1</i> was isolated from poplar (<i>P. trichocarpa</i>), and its biological functions in the regulation of biomass production and salt tolerance were investigated in transgenic poplar. <i>PtGSTF1</i> was ubiquitously expressed in various tissues and organs, with a predominant expression in leaves and inducible expression by salt stress. Transgenic poplar overexpressing <i>PtGSTF1</i> showed improved shoot growth, wood formation and improved salt tolerance, consistent with the increased xylem cell number and size under normal condition, and the optimized Na<sup>+</sup> and K<sup>+</sup> homeostasis and strengthened reactive oxygen species scavenging during salt stress. Further transcriptome analyses demonstrated that the expressions of genes related to hydrolase, cell wall modification, ion homeostasis and ROS scavenging were up- or down-regulated in transgenic plants. Our findings imply that <i>PtGSTF1</i> improves both biomass production and salt tolerance through regulating hydrolase activity, cell wall modification, ion homeostasis and ROS scavenging in transgenic poplar, and that it can be considered as a useful gene candidate for the genetic breeding of new tree varieties with improved growth under salt stress conditions. |
| first_indexed | 2024-03-09T21:40:57Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:40:57Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4644c222bc5845fd8b57a970af8f8a892023-11-23T20:30:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191128810.3390/ijms231911288The Glutathione <i>S</i>-Transferase <i>PtGSTF1</i> Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in PoplarHongsheng Gao0Chunyan Yu1Ruichao Liu2Xiaoyan Li3Huiqing Huang4Xueting Wang5Chao Zhang6Ning Jiang7Xiaofang Li8Shuang Cheng9Hongxia Zhang10Bei Li11The Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai 264025, ChinaGlutathione <i>S</i>-transferases (GSTs) play an essential role in plant cell detoxification and secondary metabolism. However, their accurate functions in the growth and response to abiotic stress in woody plants are still largely unknown. In this work, a Phi class Glutathione <i>S</i>-transferase encoding gene <i>PtGSTF1</i> was isolated from poplar (<i>P. trichocarpa</i>), and its biological functions in the regulation of biomass production and salt tolerance were investigated in transgenic poplar. <i>PtGSTF1</i> was ubiquitously expressed in various tissues and organs, with a predominant expression in leaves and inducible expression by salt stress. Transgenic poplar overexpressing <i>PtGSTF1</i> showed improved shoot growth, wood formation and improved salt tolerance, consistent with the increased xylem cell number and size under normal condition, and the optimized Na<sup>+</sup> and K<sup>+</sup> homeostasis and strengthened reactive oxygen species scavenging during salt stress. Further transcriptome analyses demonstrated that the expressions of genes related to hydrolase, cell wall modification, ion homeostasis and ROS scavenging were up- or down-regulated in transgenic plants. Our findings imply that <i>PtGSTF1</i> improves both biomass production and salt tolerance through regulating hydrolase activity, cell wall modification, ion homeostasis and ROS scavenging in transgenic poplar, and that it can be considered as a useful gene candidate for the genetic breeding of new tree varieties with improved growth under salt stress conditions.https://www.mdpi.com/1422-0067/23/19/11288glutathione <i>S</i>-transferase (GST)<i>PtGSTF1</i>reactive oxygen speciessalt stresstranscriptomic analysis |
| spellingShingle | Hongsheng Gao Chunyan Yu Ruichao Liu Xiaoyan Li Huiqing Huang Xueting Wang Chao Zhang Ning Jiang Xiaofang Li Shuang Cheng Hongxia Zhang Bei Li The Glutathione <i>S</i>-Transferase <i>PtGSTF1</i> Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar International Journal of Molecular Sciences glutathione <i>S</i>-transferase (GST) <i>PtGSTF1</i> reactive oxygen species salt stress transcriptomic analysis |
| title | The Glutathione <i>S</i>-Transferase <i>PtGSTF1</i> Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar |
| title_full | The Glutathione <i>S</i>-Transferase <i>PtGSTF1</i> Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar |
| title_fullStr | The Glutathione <i>S</i>-Transferase <i>PtGSTF1</i> Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar |
| title_full_unstemmed | The Glutathione <i>S</i>-Transferase <i>PtGSTF1</i> Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar |
| title_short | The Glutathione <i>S</i>-Transferase <i>PtGSTF1</i> Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar |
| title_sort | glutathione i s i transferase i ptgstf1 i improves biomass production and salt tolerance through regulating xylem cell proliferation ion homeostasis and reactive oxygen species scavenging in poplar |
| topic | glutathione <i>S</i>-transferase (GST) <i>PtGSTF1</i> reactive oxygen species salt stress transcriptomic analysis |
| url | https://www.mdpi.com/1422-0067/23/19/11288 |
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