Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle
RNA interference (RNAi)-based technology has been proven as a novel approach for insect pest control. However, whether insects could evolve resistance to RNAi and the underlying mechanism is largely unknown. The target gene mutations were thought to be one of the potential ways to develop the resist...
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MDPI AG
2020-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/7/449 |
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| author | Wanwan He Wenbo Xu Kaiyun Fu Wenchao Guo Jiang Zhang |
| author_facet | Wanwan He Wenbo Xu Kaiyun Fu Wenchao Guo Jiang Zhang |
| author_sort | Wanwan He |
| collection | DOAJ |
| description | RNA interference (RNAi)-based technology has been proven as a novel approach for insect pest control. However, whether insects could evolve resistance to RNAi and the underlying mechanism is largely unknown. The target gene mutations were thought to be one of the potential ways to develop the resistance. Here we predicted the effective siRNA candidates that could be derived from dsRNA against the Colorado potato beetle (CPB) <i>β-Actin</i> gene (ds<i>ACT</i>). By site-directed mutagenesis, we synthesized the dsRNAs with the defect in generation of effective siRNAs (and thus were supposed to have comparable low RNAi efficacy). We showed that, with mismatches to the target gene, all the dsRNA variants caused similar levels of silencing of target gene, mortality and larval growth retardation of CPB. Our results suggest that when the mismatch rate of ds<i>ACT</i> and target <i>β-Actin</i> mRNA is less than 3%, the RNAi efficiency is not impaired in CPB, which might imply the low possibility of RNAi resistance evolving through the sequence mismatches between dsRNA and the target gene. |
| first_indexed | 2024-03-10T18:27:31Z |
| format | Article |
| id | doaj.art-46456b0d56b94039998b526283513cb2 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T18:27:31Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-46456b0d56b94039998b526283513cb22023-11-20T06:55:01ZengMDPI AGInsects2075-44502020-07-0111744910.3390/insects11070449Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato BeetleWanwan He0Wenbo Xu1Kaiyun Fu2Wenchao Guo3Jiang Zhang4State Key Laboratory of Biocatalysis of Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, ChinaState Key Laboratory of Biocatalysis of Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, ChinaInstitute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, ChinaInstitute of Microbial Application, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, ChinaState Key Laboratory of Biocatalysis of Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, ChinaRNA interference (RNAi)-based technology has been proven as a novel approach for insect pest control. However, whether insects could evolve resistance to RNAi and the underlying mechanism is largely unknown. The target gene mutations were thought to be one of the potential ways to develop the resistance. Here we predicted the effective siRNA candidates that could be derived from dsRNA against the Colorado potato beetle (CPB) <i>β-Actin</i> gene (ds<i>ACT</i>). By site-directed mutagenesis, we synthesized the dsRNAs with the defect in generation of effective siRNAs (and thus were supposed to have comparable low RNAi efficacy). We showed that, with mismatches to the target gene, all the dsRNA variants caused similar levels of silencing of target gene, mortality and larval growth retardation of CPB. Our results suggest that when the mismatch rate of ds<i>ACT</i> and target <i>β-Actin</i> mRNA is less than 3%, the RNAi efficiency is not impaired in CPB, which might imply the low possibility of RNAi resistance evolving through the sequence mismatches between dsRNA and the target gene.https://www.mdpi.com/2075-4450/11/7/449RNA interferencedouble-stranded RNAsequence mutationresistance developmentColorado potato beetle |
| spellingShingle | Wanwan He Wenbo Xu Kaiyun Fu Wenchao Guo Jiang Zhang Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle Insects RNA interference double-stranded RNA sequence mutation resistance development Colorado potato beetle |
| title | Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle |
| title_full | Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle |
| title_fullStr | Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle |
| title_full_unstemmed | Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle |
| title_short | Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle |
| title_sort | low mismatch rate between double stranded rna and target mrna does not affect rna interference efficiency in colorado potato beetle |
| topic | RNA interference double-stranded RNA sequence mutation resistance development Colorado potato beetle |
| url | https://www.mdpi.com/2075-4450/11/7/449 |
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