Inhibition of ASCT2 is essential in all‐trans retinoic acid‐induced reduction of adipogenesis in 3T3‐L1 cells
Vitamin A has preventive effects on obesity. All‐trans retinoic acid (ATRA), the active form of vitamin A, inhibits lipid accumulation in 3T3‐L1 cells in an experimental adipogenesis model. We found that ATRA suppressed up‐regulation of the amino acid transporter, Asct2, in adipogenerating 3T3‐L1 ce...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.fob.2015.06.012 |
| _version_ | 1811185072811802624 |
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| author | Katsuhiko Takahashi Natsumi Uchida Chisato Kitanaka Chiaki Sagara Masahiko Imai Noriko Takahashi |
| author_facet | Katsuhiko Takahashi Natsumi Uchida Chisato Kitanaka Chiaki Sagara Masahiko Imai Noriko Takahashi |
| author_sort | Katsuhiko Takahashi |
| collection | DOAJ |
| description | Vitamin A has preventive effects on obesity. All‐trans retinoic acid (ATRA), the active form of vitamin A, inhibits lipid accumulation in 3T3‐L1 cells in an experimental adipogenesis model. We found that ATRA suppressed up‐regulation of the amino acid transporter, Asct2, in adipogenerating 3T3‐L1 cells. We observed that Asct2 was up‐regulated at 1 day after adipogenesis stimuli. The Asct2 inhibitorl‐γ‐glutamyl‐p‐nitroanilide (GPNA) decreased lipid accumulation. Glutamine‐free conditions also suppressed adipogenesis. Suppression of adipogenesis by ATRA may be through Asct2 reduction. These results indicate that Asct2 could be a target for obesity prevention and treatment. |
| first_indexed | 2024-04-11T13:24:31Z |
| format | Article |
| id | doaj.art-4646562bf72d4ccea9da31e18cbd3046 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T13:24:31Z |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-4646562bf72d4ccea9da31e18cbd30462022-12-22T04:22:07ZengWileyFEBS Open Bio2211-54632015-01-015157157810.1016/j.fob.2015.06.012Inhibition of ASCT2 is essential in all‐trans retinoic acid‐induced reduction of adipogenesis in 3T3‐L1 cellsKatsuhiko Takahashi0Natsumi Uchida1Chisato Kitanaka2Chiaki Sagara3Masahiko Imai4Noriko Takahashi5Laboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo 142-8501, JapanLaboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo 142-8501, JapanLaboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo 142-8501, JapanLaboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo 142-8501, JapanLaboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo 142-8501, JapanLaboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo 142-8501, JapanVitamin A has preventive effects on obesity. All‐trans retinoic acid (ATRA), the active form of vitamin A, inhibits lipid accumulation in 3T3‐L1 cells in an experimental adipogenesis model. We found that ATRA suppressed up‐regulation of the amino acid transporter, Asct2, in adipogenerating 3T3‐L1 cells. We observed that Asct2 was up‐regulated at 1 day after adipogenesis stimuli. The Asct2 inhibitorl‐γ‐glutamyl‐p‐nitroanilide (GPNA) decreased lipid accumulation. Glutamine‐free conditions also suppressed adipogenesis. Suppression of adipogenesis by ATRA may be through Asct2 reduction. These results indicate that Asct2 could be a target for obesity prevention and treatment.https://doi.org/10.1016/j.fob.2015.06.012AdipocyteAll-trans retinoic acidAsct2l-glutamineObesity |
| spellingShingle | Katsuhiko Takahashi Natsumi Uchida Chisato Kitanaka Chiaki Sagara Masahiko Imai Noriko Takahashi Inhibition of ASCT2 is essential in all‐trans retinoic acid‐induced reduction of adipogenesis in 3T3‐L1 cells FEBS Open Bio Adipocyte All-trans retinoic acid Asct2 l-glutamine Obesity |
| title | Inhibition of ASCT2 is essential in all‐trans retinoic acid‐induced reduction of adipogenesis in 3T3‐L1 cells |
| title_full | Inhibition of ASCT2 is essential in all‐trans retinoic acid‐induced reduction of adipogenesis in 3T3‐L1 cells |
| title_fullStr | Inhibition of ASCT2 is essential in all‐trans retinoic acid‐induced reduction of adipogenesis in 3T3‐L1 cells |
| title_full_unstemmed | Inhibition of ASCT2 is essential in all‐trans retinoic acid‐induced reduction of adipogenesis in 3T3‐L1 cells |
| title_short | Inhibition of ASCT2 is essential in all‐trans retinoic acid‐induced reduction of adipogenesis in 3T3‐L1 cells |
| title_sort | inhibition of asct2 is essential in all trans retinoic acid induced reduction of adipogenesis in 3t3 l1 cells |
| topic | Adipocyte All-trans retinoic acid Asct2 l-glutamine Obesity |
| url | https://doi.org/10.1016/j.fob.2015.06.012 |
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