EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYST
In an attempt to produce the interspecies embryo transfer, this study was conducted to evaluate the efficacy of the production of reconstructed blastocyst by transferring the donor ICM into the recipient trophoblast. ICM cells were isolated from the donor blastocyst by immunosurgery method. Zona-fre...
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| Format: | Article |
| Language: | English |
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SEAMEO, BIOTROP
2011-08-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Online Access: | https://journal.biotrop.org/index.php/biotropia/article/view/222 |
| _version_ | 1797758563930603520 |
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| author | ARIEF BOEDIONO |
| author_facet | ARIEF BOEDIONO |
| author_sort | ARIEF BOEDIONO |
| collection | DOAJ |
| description | In an attempt to produce the interspecies embryo transfer, this study was conducted to evaluate the efficacy of the production of reconstructed blastocyst by transferring the donor ICM into the recipient trophoblast. ICM cells were isolated from the donor blastocyst by immunosurgery method. Zona-free blastocysts were incubated in the medium (TCM-199) containing 20% of the heat-inactivated rabbit anti-bovine-serum. The embryo reconstruction was produced by three different methods. Recipient blastocyst was maintained on the holding pipette by gentle suction, with the ICM in a 9 o'clock position to have the possibility of developing incorporate ICMs (Method I), the ICM was in a 3 o'clock position to break the original ICM during injection (Method 2); cutting the original recipient ICM followed by insertion of the donor ICM (Method 3). Reconstructed blastocysts were then cultured overnight and examined morphologically according to the re-expansion of the reconstructed blastocyst with or without developed donor ICM. According to morphological observation in this study, 37.9% of the reconstructed blastocyst developed with the incorporation of two ICM originally from recipient and donor (Method I), 66.7% of the reconstructed blastocysts developed with a single ICM (Method 2), and 80.0% of the reconstructed blastocyst developed from the ICM originally from donor ICM (Method 3). These results showed that the reconstructed blastocyst is better produced by cutting the original recipient ICM followed by the insertion of the donor ICM (Method 3).
Key words: embryo reconstruction, immunosurgery, ICM transfer, bovine. |
| first_indexed | 2024-03-12T18:31:48Z |
| format | Article |
| id | doaj.art-4647f1a1dd7f4f67bdbb4232429ded7c |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-03-12T18:31:48Z |
| publishDate | 2011-08-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-4647f1a1dd7f4f67bdbb4232429ded7c2023-08-02T08:15:54ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2011-08-0113210.11598/btb.2006.13.2.222EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYSTARIEF BOEDIONO0Laboratory of Embryology, Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural UniversityIn an attempt to produce the interspecies embryo transfer, this study was conducted to evaluate the efficacy of the production of reconstructed blastocyst by transferring the donor ICM into the recipient trophoblast. ICM cells were isolated from the donor blastocyst by immunosurgery method. Zona-free blastocysts were incubated in the medium (TCM-199) containing 20% of the heat-inactivated rabbit anti-bovine-serum. The embryo reconstruction was produced by three different methods. Recipient blastocyst was maintained on the holding pipette by gentle suction, with the ICM in a 9 o'clock position to have the possibility of developing incorporate ICMs (Method I), the ICM was in a 3 o'clock position to break the original ICM during injection (Method 2); cutting the original recipient ICM followed by insertion of the donor ICM (Method 3). Reconstructed blastocysts were then cultured overnight and examined morphologically according to the re-expansion of the reconstructed blastocyst with or without developed donor ICM. According to morphological observation in this study, 37.9% of the reconstructed blastocyst developed with the incorporation of two ICM originally from recipient and donor (Method I), 66.7% of the reconstructed blastocysts developed with a single ICM (Method 2), and 80.0% of the reconstructed blastocyst developed from the ICM originally from donor ICM (Method 3). These results showed that the reconstructed blastocyst is better produced by cutting the original recipient ICM followed by the insertion of the donor ICM (Method 3). Key words: embryo reconstruction, immunosurgery, ICM transfer, bovine.https://journal.biotrop.org/index.php/biotropia/article/view/222 |
| spellingShingle | ARIEF BOEDIONO EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYST Biotropia: The Southeast Asian Journal of Tropical Biology |
| title | EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYST |
| title_full | EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYST |
| title_fullStr | EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYST |
| title_full_unstemmed | EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYST |
| title_short | EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYST |
| title_sort | embryo reconstruction by transplantation of the donor inner cell mass to the recipient bovine blastocyst |
| url | https://journal.biotrop.org/index.php/biotropia/article/view/222 |
| work_keys_str_mv | AT ariefboediono embryoreconstructionbytransplantationofthedonorinnercellmasstotherecipientbovineblastocyst |