Probiotic-Induced Modulation of Microbiota Composition and Antibiotic Resistance Genes Load, an In Vitro Assessment
The imbalance of the gut microbiota (GM) is known as dysbiosis and is associated with disorders such as obesity. The increasing prevalence of microorganisms harboring antibiotic resistance genes (ARG) in the GM has been reported as a potential risk for spreading multi-drug-resistant pathogens. The o...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/2/1003 |
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| author | Alicja Maria Nogacka Silvia Saturio Guadalupe Monserrat Alvarado-Jasso Nuria Salazar Clara G. de los Reyes Gavilán Ceferino Martínez-Faedo Adolfo Suarez Ruipeng Wang Kenji Miyazawa Gaku Harata Akihito Endo Silvia Arboleya Miguel Gueimonde |
| author_facet | Alicja Maria Nogacka Silvia Saturio Guadalupe Monserrat Alvarado-Jasso Nuria Salazar Clara G. de los Reyes Gavilán Ceferino Martínez-Faedo Adolfo Suarez Ruipeng Wang Kenji Miyazawa Gaku Harata Akihito Endo Silvia Arboleya Miguel Gueimonde |
| author_sort | Alicja Maria Nogacka |
| collection | DOAJ |
| description | The imbalance of the gut microbiota (GM) is known as dysbiosis and is associated with disorders such as obesity. The increasing prevalence of microorganisms harboring antibiotic resistance genes (ARG) in the GM has been reported as a potential risk for spreading multi-drug-resistant pathogens. The objective of this work was the evaluation, in a fecal culture model, of different probiotics for their ability to modulate GM composition and ARG levels on two population groups, extremely obese (OB) and normal-weight (NW) subjects. Clear differences in the basal microbiota composition were observed between NW and OB donors. The microbial profile assessed by metataxonomics revealed the broader impact of probiotics on the OB microbiota composition. Also, supplementation with probiotics promoted significant reductions in the absolute levels of <i>tetM</i> and <i>tetO</i> genes. Regarding the <i>blaTEM</i> gene, a minor but significant decrease in both donor groups was detected after probiotic addition. A negative association between the abundance of Bifidobacteriaceae and the <i>tetM</i> gene was observed. Our results show the ability of some of the tested strains to modulate GM. Moreover, the results suggest the potential application of probiotics for reducing the levels of ARG, which constitutes an interesting target for the future development of probiotics. |
| first_indexed | 2024-03-08T09:53:42Z |
| format | Article |
| id | doaj.art-4649cebdadd743dc9306e1cf3bc14555 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:53:42Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4649cebdadd743dc9306e1cf3bc145552024-01-29T13:56:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01252100310.3390/ijms25021003Probiotic-Induced Modulation of Microbiota Composition and Antibiotic Resistance Genes Load, an In Vitro AssessmentAlicja Maria Nogacka0Silvia Saturio1Guadalupe Monserrat Alvarado-Jasso2Nuria Salazar3Clara G. de los Reyes Gavilán4Ceferino Martínez-Faedo5Adolfo Suarez6Ruipeng Wang7Kenji Miyazawa8Gaku Harata9Akihito Endo10Silvia Arboleya11Miguel Gueimonde12Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), 33300 Villaviciosa, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), 33300 Villaviciosa, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), 33300 Villaviciosa, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), 33300 Villaviciosa, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), 33300 Villaviciosa, SpainEndocrinology and Nutrition Service, Central University Hospital of Asturias (HUCA), 33011 Oviedo, SpainDiet, Microbiota and Health Group, Instituto de Investigación Sanitaria del Principado de Asturias (DIMISA-ISPA), 33011 Oviedo, SpainTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0021, JapanTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0021, JapanTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0021, JapanDepartment of Food, Aroma and Cosmetic Chemistry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), 33300 Villaviciosa, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), 33300 Villaviciosa, SpainThe imbalance of the gut microbiota (GM) is known as dysbiosis and is associated with disorders such as obesity. The increasing prevalence of microorganisms harboring antibiotic resistance genes (ARG) in the GM has been reported as a potential risk for spreading multi-drug-resistant pathogens. The objective of this work was the evaluation, in a fecal culture model, of different probiotics for their ability to modulate GM composition and ARG levels on two population groups, extremely obese (OB) and normal-weight (NW) subjects. Clear differences in the basal microbiota composition were observed between NW and OB donors. The microbial profile assessed by metataxonomics revealed the broader impact of probiotics on the OB microbiota composition. Also, supplementation with probiotics promoted significant reductions in the absolute levels of <i>tetM</i> and <i>tetO</i> genes. Regarding the <i>blaTEM</i> gene, a minor but significant decrease in both donor groups was detected after probiotic addition. A negative association between the abundance of Bifidobacteriaceae and the <i>tetM</i> gene was observed. Our results show the ability of some of the tested strains to modulate GM. Moreover, the results suggest the potential application of probiotics for reducing the levels of ARG, which constitutes an interesting target for the future development of probiotics.https://www.mdpi.com/1422-0067/25/2/1003gut microbiotafecal cultureprobioticsextreme obesityantibiotic resistance genes |
| spellingShingle | Alicja Maria Nogacka Silvia Saturio Guadalupe Monserrat Alvarado-Jasso Nuria Salazar Clara G. de los Reyes Gavilán Ceferino Martínez-Faedo Adolfo Suarez Ruipeng Wang Kenji Miyazawa Gaku Harata Akihito Endo Silvia Arboleya Miguel Gueimonde Probiotic-Induced Modulation of Microbiota Composition and Antibiotic Resistance Genes Load, an In Vitro Assessment International Journal of Molecular Sciences gut microbiota fecal culture probiotics extreme obesity antibiotic resistance genes |
| title | Probiotic-Induced Modulation of Microbiota Composition and Antibiotic Resistance Genes Load, an In Vitro Assessment |
| title_full | Probiotic-Induced Modulation of Microbiota Composition and Antibiotic Resistance Genes Load, an In Vitro Assessment |
| title_fullStr | Probiotic-Induced Modulation of Microbiota Composition and Antibiotic Resistance Genes Load, an In Vitro Assessment |
| title_full_unstemmed | Probiotic-Induced Modulation of Microbiota Composition and Antibiotic Resistance Genes Load, an In Vitro Assessment |
| title_short | Probiotic-Induced Modulation of Microbiota Composition and Antibiotic Resistance Genes Load, an In Vitro Assessment |
| title_sort | probiotic induced modulation of microbiota composition and antibiotic resistance genes load an in vitro assessment |
| topic | gut microbiota fecal culture probiotics extreme obesity antibiotic resistance genes |
| url | https://www.mdpi.com/1422-0067/25/2/1003 |
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