Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, Libya
Background: Vibrios are aquatic bacteria and ubiquitous in the marine environment causing Vibriosis in humans and aquatic animals. Vibriosis is a significant disease of cultured and wild fish living in marine water, brackish, and freshwater, which causes highly economic losses. Aim: The present st...
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| Format: | Article |
| Language: | English |
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Tripoli University
2023-06-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=64965 |
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| author | Khawla K. Bukha Shaima A. M. Mahgiubi Asma M. Elbahi Ehab A. Sharif Mohamed L. Showehdi Mohamed O. Ahmed Abdulwahab M. kammon Yousef M. Abouzeed |
| author_facet | Khawla K. Bukha Shaima A. M. Mahgiubi Asma M. Elbahi Ehab A. Sharif Mohamed L. Showehdi Mohamed O. Ahmed Abdulwahab M. kammon Yousef M. Abouzeed |
| author_sort | Khawla K. Bukha |
| collection | DOAJ |
| description | Background:
Vibrios are aquatic bacteria and ubiquitous in the marine environment causing Vibriosis in humans and aquatic animals. Vibriosis is a significant disease of cultured and wild fish living in marine water, brackish, and freshwater, which causes highly economic losses.
Aim:
The present study was aimed to address the impact of Vibrios on the health status of Trachurus trachurus inhabiting coastal area of Tripoli.
Methods:
A total of 100 samples of (Trachurus trachurus) were collected randomly from the Western Coast of Tripoli and Bab Al- Baher market, throughout the period from spring 2019 to summer 2019.All the sampled fish were examined externally and internally to identify any potential lesions. Bacterial isolation from liver and kidney was carried out using the appropriate culture media. Tissue samples were taken from liver, kidney and spleen in 10% neutral buffered formalin for histopathology examination. The tissue sections were stained with Hematoxylin and Eosin (H&E) to assess morphologically and Perl's Prussian blue for the demonstration of ferric iron.
Results:
On average 69% of the infected fish exhibited at least one pathological lesion. Vibrios were recovered from 90% of the examined fishes. The histopathological changes in the liver were observed as severe congestion of blood vessels, mononuclear cell infiltration surrounding a bile duct, granular degeneration and coagulative necrosis of hepatocytes in the centrilobular area, marked vacuolar degeneration of hepatocytes, activation of melano-macrophages centers (MMCs), multiple cysts of nematode in hepatic tissue (incidental finding). With respect to kidney and spleen, examination of H & E stained tissue sections have severe congestion of blood vessels, vacuolar degeneration of the renal tubular epithelium, severe interstitial mononuclear cell infiltration, and marked activation of melano-macrophages centers (MMCs) in the kidney. Moreover, congestion of blood vessels and marked activation of melano-macrophages (MMCs) were found in the spleen. The melano-macrophage centers (MMCs) of most of the sampled tissues showed a strong positive reaction for ferric iron.
Conclusion:
The polluted aquatic environment by sewage at Tripoli Coast is an essential factor for triggering the pathogenicity and invasion of Vibrios to vulnerable Atlantic horse mackerel fishes. Also, this study are a preliminary step to give a baseline for further future studies on epidemiology and control of Vibrio infection in Libyan fish. [Open Vet J 2023; 13(3.000): 327-336] |
| first_indexed | 2024-04-09T20:15:14Z |
| format | Article |
| id | doaj.art-464c1b10fe264da88a3d19038ec2fe68 |
| institution | Directory Open Access Journal |
| issn | 2226-4485 |
| language | English |
| last_indexed | 2024-04-09T20:15:14Z |
| publishDate | 2023-06-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-464c1b10fe264da88a3d19038ec2fe682023-03-31T10:18:09ZengTripoli UniversityOpen Veterinary Journal2226-44852023-06-0113332733610.5455/OVJ.2023.v13.i3.964965Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, LibyaKhawla K. Bukha0Shaima A. M. MahgiubiAsma M. ElbahiEhab A. SharifMohamed L. ShowehdiMohamed O. AhmedAbdulwahab M. kammonYousef M. AbouzeedDepartment of Microbiology and Parasitology Faculty of Veterinary Medicine, University of Tripoli, Libya.Background: Vibrios are aquatic bacteria and ubiquitous in the marine environment causing Vibriosis in humans and aquatic animals. Vibriosis is a significant disease of cultured and wild fish living in marine water, brackish, and freshwater, which causes highly economic losses. Aim: The present study was aimed to address the impact of Vibrios on the health status of Trachurus trachurus inhabiting coastal area of Tripoli. Methods: A total of 100 samples of (Trachurus trachurus) were collected randomly from the Western Coast of Tripoli and Bab Al- Baher market, throughout the period from spring 2019 to summer 2019.All the sampled fish were examined externally and internally to identify any potential lesions. Bacterial isolation from liver and kidney was carried out using the appropriate culture media. Tissue samples were taken from liver, kidney and spleen in 10% neutral buffered formalin for histopathology examination. The tissue sections were stained with Hematoxylin and Eosin (H&E) to assess morphologically and Perl's Prussian blue for the demonstration of ferric iron. Results: On average 69% of the infected fish exhibited at least one pathological lesion. Vibrios were recovered from 90% of the examined fishes. The histopathological changes in the liver were observed as severe congestion of blood vessels, mononuclear cell infiltration surrounding a bile duct, granular degeneration and coagulative necrosis of hepatocytes in the centrilobular area, marked vacuolar degeneration of hepatocytes, activation of melano-macrophages centers (MMCs), multiple cysts of nematode in hepatic tissue (incidental finding). With respect to kidney and spleen, examination of H & E stained tissue sections have severe congestion of blood vessels, vacuolar degeneration of the renal tubular epithelium, severe interstitial mononuclear cell infiltration, and marked activation of melano-macrophages centers (MMCs) in the kidney. Moreover, congestion of blood vessels and marked activation of melano-macrophages (MMCs) were found in the spleen. The melano-macrophage centers (MMCs) of most of the sampled tissues showed a strong positive reaction for ferric iron. Conclusion: The polluted aquatic environment by sewage at Tripoli Coast is an essential factor for triggering the pathogenicity and invasion of Vibrios to vulnerable Atlantic horse mackerel fishes. Also, this study are a preliminary step to give a baseline for further future studies on epidemiology and control of Vibrio infection in Libyan fish. [Open Vet J 2023; 13(3.000): 327-336]http://www.ejmanager.com/fulltextpdf.php?mno=64965atlantic horse mackerel fishvibrionaceaehistopathological changesmelano-macrophages centers |
| spellingShingle | Khawla K. Bukha Shaima A. M. Mahgiubi Asma M. Elbahi Ehab A. Sharif Mohamed L. Showehdi Mohamed O. Ahmed Abdulwahab M. kammon Yousef M. Abouzeed Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, Libya Open Veterinary Journal atlantic horse mackerel fish vibrionaceae histopathological changes melano-macrophages centers |
| title | Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, Libya |
| title_full | Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, Libya |
| title_fullStr | Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, Libya |
| title_full_unstemmed | Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, Libya |
| title_short | Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, Libya |
| title_sort | pathological lesions associated with vibrio infection in atlantic horse mackerel trachurus trachurus l 1758 from the western coast of tripoli libya |
| topic | atlantic horse mackerel fish vibrionaceae histopathological changes melano-macrophages centers |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=64965 |
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