Evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana (Musa spp.)
To identify symbiosis between roots and arbuscular mycorrhizal fungi (HMA), methodologies have been reported with variations in concentrations of reagents, temperature, and time of exposure for staining, which does not always adjust to the need for specific cultures due to variation in root structur...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Spanish |
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Universidad de Córdoba
2022-07-01
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| Series: | Temas Agrarios |
| Subjects: | |
| Online Access: | https://revistas.unicordoba.edu.co/index.php/temasagrarios/article/view/3134/5172 |
| _version_ | 1797202382365392896 |
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| author | Miguel Angel Bernal Monterrosa Laura Delgado Bejarano Sebastián Zapata Henao Ever José Causil Pastrana |
| author_facet | Miguel Angel Bernal Monterrosa Laura Delgado Bejarano Sebastián Zapata Henao Ever José Causil Pastrana |
| author_sort | Miguel Angel Bernal Monterrosa |
| collection | DOAJ |
| description | To identify symbiosis between roots and arbuscular mycorrhizal fungi (HMA), methodologies have been reported with variations in concentrations of reagents, temperature, and time of exposure for staining, which does not always adjust to the need for specific cultures due to variation in root structures. The objective of this research was to standardize a methodology to identify mycorrhizal colonization in banana crops in an efficient way. The research was carried out during the second semester of 2022 in the municipality of Apartadó-Antioquia. Four treatments were established that consisted of the evaluation with and without mycorrhizal inoculation with two bleaching techniques each (with and without subjection to Bain Marie). A total of 108 plates (54/technique) were evaluated, where percentage of colonization, visibility, vesicles and arbuscules were identified. There was a complete randomized design. Data were analyzed with R Studio 2022.02 software. An ANAVA and means separations were obtained with a post-hoc analysis of Tukey and Kruskal-Wallis with a confidence level of 95%. Symbiosis was found in all cases, statistical differences were identified in percentage of colonization with 46.8% to 90.2%; arbuscules from 12.1 to 338.0 units and a visibility percentage of 100% in treatments subjected to a Bain Marie, highlighting the need for subjecting banana roots to heat to favor discoloration, staining and visibility due to their architecture. |
| first_indexed | 2024-04-24T08:02:33Z |
| format | Article |
| id | doaj.art-464ce43b284d41e9a9ecff8edd3cec73 |
| institution | Directory Open Access Journal |
| issn | 2389-9182 |
| language | Spanish |
| last_indexed | 2024-04-24T08:02:33Z |
| publishDate | 2022-07-01 |
| publisher | Universidad de Córdoba |
| record_format | Article |
| series | Temas Agrarios |
| spelling | doaj.art-464ce43b284d41e9a9ecff8edd3cec732024-04-17T15:48:42ZspaUniversidad de CórdobaTemas Agrarios2389-91822022-07-0127238539610.21897/rta.v27i2.3134Evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana (Musa spp.) Miguel Angel Bernal Monterrosa0Laura Delgado Bejarano1Sebastián Zapata Henao2Ever José Causil Pastrana3Centro de investigaciones del banano - Cenibanano, Carepa – Colombia. Centro de investigaciones del banano - Cenibanano, Carepa – Colombia. Centro de investigaciones del banano - Cenibanano, Carepa – Colombia. Centro de investigaciones del banano - Cenibanano, Carepa – Colombia.To identify symbiosis between roots and arbuscular mycorrhizal fungi (HMA), methodologies have been reported with variations in concentrations of reagents, temperature, and time of exposure for staining, which does not always adjust to the need for specific cultures due to variation in root structures. The objective of this research was to standardize a methodology to identify mycorrhizal colonization in banana crops in an efficient way. The research was carried out during the second semester of 2022 in the municipality of Apartadó-Antioquia. Four treatments were established that consisted of the evaluation with and without mycorrhizal inoculation with two bleaching techniques each (with and without subjection to Bain Marie). A total of 108 plates (54/technique) were evaluated, where percentage of colonization, visibility, vesicles and arbuscules were identified. There was a complete randomized design. Data were analyzed with R Studio 2022.02 software. An ANAVA and means separations were obtained with a post-hoc analysis of Tukey and Kruskal-Wallis with a confidence level of 95%. Symbiosis was found in all cases, statistical differences were identified in percentage of colonization with 46.8% to 90.2%; arbuscules from 12.1 to 338.0 units and a visibility percentage of 100% in treatments subjected to a Bain Marie, highlighting the need for subjecting banana roots to heat to favor discoloration, staining and visibility due to their architecture.https://revistas.unicordoba.edu.co/index.php/temasagrarios/article/view/3134/5172standardize methodologymusaceaecolonization percentagewater bathclearingstaining |
| spellingShingle | Miguel Angel Bernal Monterrosa Laura Delgado Bejarano Sebastián Zapata Henao Ever José Causil Pastrana Evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana (Musa spp.) Temas Agrarios standardize methodology musaceae colonization percentage water bath clearing staining |
| title | Evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana (Musa spp.) |
| title_full | Evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana (Musa spp.) |
| title_fullStr | Evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana (Musa spp.) |
| title_full_unstemmed | Evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana (Musa spp.) |
| title_short | Evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana (Musa spp.) |
| title_sort | evaluation of two root bleaching techniques to determine mycorrhizal symbiosis in banana musa spp |
| topic | standardize methodology musaceae colonization percentage water bath clearing staining |
| url | https://revistas.unicordoba.edu.co/index.php/temasagrarios/article/view/3134/5172 |
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